John C Tabone

US Patent:

6361940, Mar 26, 2002

Filed:

Apr 1, 1998

Appl. No.:

09/053831

Inventors:

Jeffrey Van Ness - Seattle WA
John C. Tabone - Bothell WA
Lori K. Garrison - Seattle WA

Assignee:

QIAGEN Genomics, Inc. - Bothell WA

International Classification:

C12Q 168

US Classification:

435 6, 435 911, 435 912, 536 221, 536 231, 536 243, 536 253

Abstract:

Compositions and methods are provided for increasing the specificity of a probe nucleic acid for a target nucleic acid in a hybridization solution. An abasic residue, deoxyNebularine residue, or a hybotrope is used to increase specificity. A method is provided for identifying useful hybotropes, including salts, water miscible organic solvents, aprotic solvents and organic solvents, on the basis of enthalpy considerations. Hybotropic hybridization and modified oligonucleotides may be used in amplification reactions, such as PCR, sequence analysis methods, and genomic screening methods.

US Patent:

6365349, Apr 2, 2002

Filed:

Jul 21, 1998

Appl. No.:

09/120689

Inventors:

Kristen Moynihan - Seattle WA
Jeffrey Van Ness - Seattle WA
John C. Tabone - Bothell WA

Assignee:

Qiagen Genomics, Inc. - Bothell WA

International Classification:

C12Q 168

US Classification:

435 6, 4352872, 536 231

Abstract:

A method for depositing biomolecule onto a solid support, the method including the steps of: immersing a tip of a spring probe into a solution of biomolecule; removing said tip from said solution to provide biomolecule solution adhered to said tip; and contacting said biomolecule solution with a solid support to thereby transfer biomolecule solution from said tip to said solid support. The spring probe has a planar tip but it otherwise identical to commercial spring probes. The solution of biomolecule contains a thickening agent in addition to biomolecule, where oligonucleotide is a preferred biomolecule.

US Patent:

6444422, Sep 3, 2002

Filed:

Jul 21, 1998

Appl. No.:

09/120686

Inventors:

Jeffrey Van Ness - Seattle WA
John C. Tabone - Bothell WA
J. Jeffry Howbert - Bellevue WA
John T. Mulligan - Seattle WA

Assignee:

Qiagen Genomics, Inc. - Bothell WA

International Classification:

C12Q 168

US Classification:

435 6, 435 711, 4352872, 4352887, 436173, 536 253, 536 254, 935 77, 935 78, 702 20, 703 11

Abstract:

A method and system for correlating characteristics (e. g. , type of nucleotide) of biomolecules (e. g. , DNA) to molecular tags with unique molecular weights that are associated with the biomolecule. In one embodiment. the molecular tags are applied to primers used when synthesizing the biomolecule. The system initially receives a mapping of each characteristic of the biomolecules to the corresponding molecular weight of the molecular tag. The system also receives an indication of the molecular weights detected when analyzing the biomolecules to which the molecular tags have been associated. For each molecular weight detected, the system determines based on the received mapping the characteristic corresponding to the detected molecular weight. The system then indicates that the analyzed biomolecule has the determined characteristic.

US Patent:

6613508, Sep 2, 2003

Filed:

Jul 22, 1997

Appl. No.:

08/898564

Inventors:

Jeffrey Van Ness - Seattle WA
John C. Tabone - Bothell WA
J. Jeffry Howbert - Bellevue WA
John T. Mulligan - Seattle WA

Assignee:

Qiagen Genomics, Inc. - Bothell WA

International Classification:

C12Q 168

US Classification:

435 6

Abstract:

Tags and linkers specifically designed for a wide variety of nucleic acid reactions are disclosed, which are suitable for a wide variety of nucleic acid reactions wherein separation of nucleic acid molecules based upon size is required.

US Patent:

6623928, Sep 23, 2003

Filed:

May 14, 2001

Appl. No.:

09/855999

Inventors:

Jeffrey Van Ness - Seattle WA
John C. Tabone - Bothell WA
J. Jeffry Howbert - Bellevue WA
John T. Mulligan - Seattle WA

Assignee:

QIAGEN Genomics, Inc. - Bothell WA

International Classification:

C12Q 168

US Classification:

435 6, 436173, 536 231, 536 266

Abstract:

Methods and compounds, including compositions therefrom, are provided for determining the sequence of nucleic acid molecules. The methods permit the determination of multiple nucleic acid sequences simultaneously. The compounds are used as tags to generate tagged nucleic acid fragments which are complementary to a selected target nucleic acid molecule. Each tag is correlative with a particular nucleotide and, in a preferred embodiment, is detectable by mass spectrometry. Following separation of the tagged fragments by sequential length, the tags are cleaved from the tagged fragments. In a preferred embodiment, the tags are detected by mass spectrometry and the sequence of the nucleic acid molecule is determined therefrom. The individual steps of the methods can be used in automated format, e. g. , by the incorporation into systems.

US Patent:

6664112, Dec 16, 2003

Filed:

Jun 1, 2001

Appl. No.:

09/872761

Inventors:

John T. Mulligan - Seattle WA
John C. Tabone - Bothell WA

Assignee:

Blue Heron Biotechnology, Inc. - Bothell WA

International Classification:

G01N 3300

US Classification:

436 94, 435 6, 435 911, 536 231, 536 243, 536 2433

Abstract:

Synthetic oligonucleotides, such as synthetic DNA, often contain sequence errors due to synthetic failures (e. g. , side products and/or truncated products). Methods are provided herein for improving the sequence fidelity of synthetic double-stranded oligonucleotides by separative depletion of synthetic failures. Separation is effected by utilization of methodologies in a preparative mode under denaturing conditions. A preferred use of the methods relates to gene synthesis.

US Patent:

RE38416, Feb 3, 2004

Filed:

Oct 19, 2000

Appl. No.:

09/693213

Inventors:

Charles R. Petrie - Woodinville WA
Rich B. Meyer - Redwood CA
John C. Tabone - Bothell WA
Gerald D. Hurst - Iowa City IA

Assignee:

Epoch Biosciences, Inc. - Bothell WA

International Classification:

C07H 1904

US Classification:

536 261, 536 267, 536 268, 536 2612, 536 2613, 536 2614, 536 276, 536 278, 536 2781, 536 285, 536 2854, 536 245

Abstract:

This invention is directed to novel substituted nucleotide bases with a crosslinking arm which accomplish crosslinking between specific sites on adjoining strands of oligonucleotides a oligodeoxynucleotides. The invention is also directed to oligonucleotides comprising at least one of these crosslinking agents and to the use of the resulting novel oligonucleotides for diagnostic and therapeutic purposes. The crosslinking agents of the invention are of the following formula ( ): wherein, R is hydrogen, or a sugar moiety or analog thereof optionally substituted at its 3â or its 5â position with a phosphorus derivative attached to the sugar moiety by an oxygen and including groups Q Q and Q or with a reactive precursor thereof suitable for nucleotide bond formation; Q is hydroxy phosphate a diphosphate; Q of or S; Q is CH âRâ, SâRâ, OâRâ, or NâRâRâ; each of Râ and Râ is independently hydrogen or C alkyl; B is a nucleic acid base or analog thereof that is a component of an oligonucleotide; Y is a functional linking group; each of to and q is independently 0 to 8, inclusive; r is 0 or 1; and Aâ is a leaving group. This invention is also directed to novel 3,4-disubstituted and 3,4,-trisubstituted pyrazolo[3,4- ]-pyrimidines and to the use of these nucleic acid bases in the preparation of oligonucleotides. The invention includes nucleosides and mono- and oligonucleotides comprising at least one of these pyrazolopyrimidines, and to the use of the resulting novel oligonucleotides for diagnostic purposes.

US Patent:

6815212, Nov 9, 2004

Filed:

Oct 24, 2001

Appl. No.:

10/000467

Inventors:

Jeffrey Van Ness - Seattle WA
John C. Tabone - Bothell WA
J. Jeffry Howbert - Bellevue WA
John T. Mulligan - Seattle WA

Assignee:

Qiagen Genomics, Inc. - Bothell WA

International Classification:

G01N 2400

US Classification:

436173, 435 6, 530300, 530350, 514 23, 536 172, 560 1, 560 8

Abstract:

Methods are provided for detecting the binding of a first member to a second member of a ligand pair, comprising the steps of (a) combining a set of first tagged members with a biological sample which may contain one or more second members, under conditions, and for a time sufficient to permit binding of a first member to a second member, wherein said tag is correlative with a particular first member and detectable by non-fluorescent spectrometry, or potentiometry, (b) separating bound first and second members from unbound members, (c) cleaving the tag from the tagged first member, and (d) detecting the tag by non-fluorescent spectrometry, or potentiometry, and therefrom detecting the binding of the first member to the second member.