Jeffrey A Karg

US Patent:

6479298, Nov 12, 2002

Filed:

Dec 3, 1999

Appl. No.:

09/454988

Inventors:

Henry F. Miller - Clifton NJ
Fu-Chung Lin - Wayne NJ
Robert J. Losada - Astoria NY
Jeffrey P. Radziunas - Wallingford CT
Jeffrey Karg - Hopkinton MA

Assignee:

Becton, Dickinson and Company - Franklin Lakes NJ

International Classification:

G01N 118

US Classification:

436177, 436174, 422101, 210121, 210122

Abstract:

A device and method for separating heavier and lighter fractions of a fluid sample. The device includes a plurality of constituents comprising a container and a composite element in the container. The composite element is a separator comprising at least two components and more particularly, a bellows with a seal body, a low-density float and a high-density ballast. A fluid sample is delivered to the container and the device is subjected to centrifugation whereby the centrifugal load causes the seal body of the separator to deform so that the separator migrates through the fluid sample and then stabilizes between the heavier and lighter fractions of the fluid sample. The seal body of the separator will resiliently return to its initial configuration upon termination of the centrifugal load such that the seal body sealingly engages the container and the composite element separates the heavier and lighter fractions of the fluid sample.

US Patent:

6497325, Dec 24, 2002

Filed:

Dec 3, 1999

Appl. No.:

09/454267

Inventors:

Paul DiCesare - Norwalk CT
Jeffrey Karg - Hopkinton MA
Fu-Chung Lin - Wayne NJ

Assignee:

Becton Dickinson and Company - Franklin Lakes NJ

International Classification:

B01D 2126

US Classification:

210516, 210513, 210518

Abstract:

A device and method is provided for separating heavier and lighter fractions of a fluid sample. The device includes a plurality of constituents. The constituents include a container, a liner in the container and a composite element in the liner for separating the fractions of a fluid sample. The separator comprises a specific density at a target density range as defined by separable fluid components densities. A fluid sample is delivered to the liner and then the device is subjected to centrifugation whereby the centrifugal load causes the liner to deform and the composite element migrates into the fluid sample and stabilizes between the heavier and lighter fractions of the fluid sample. The liner will resiliently return to its initial configuration upon termination of the centrifugal load such that the liner sealingly engages the composite element and separates the heavier and lighter fractions of the fluid sample.

US Patent:

6516953, Feb 11, 2003

Filed:

Dec 2, 1999

Appl. No.:

09/452981

Inventors:

Paul C. DiCesare - Norwalk CT
Jeffrey Karg - Hopkinton MA
Fu-Chung Lin - Wayne NJ
Jeffrey P. Radziunas - Wallingford CT

Assignee:

Becton, Dickinson and Company - Franklin Lakes NJ

International Classification:

B01D 2126

US Classification:

210516, 210513, 210518

Abstract:

A device and method is provided for separating components of a fluid sample. The device includes a plurality of constituents comprising a container, a liner in the container, a closure for the container and a composite element. The composite element is a seal plug with a density between the densities of the components of the fluid sample is releasably engaged with the container closure and with the liner. A needle cannula is used to deposit a fluid sample in the liner and the entire device is placed in a centrifuge. The centrifugal load causes the seal plug to separate from the closure and causes the liner to expand outwardly against the container. The seal plug migrates into the fluid sample and stabilizes between the densities of the components of the fluid sample. The liner will resiliently return to its initial configuration upon termination of centrifugal load such that the liner sealingly engages the seal plug and separates the components of the fluid sample.

US Patent:

6620383, Sep 16, 2003

Filed:

Jun 12, 2000

Appl. No.:

09/591803

Inventors:

Jeffrey A. Karg - Hopkinton MA
Douglas W. Kroncke - Boston MA

Assignee:

Boston Innovation Inc. - MA

International Classification:

B01L 302

US Classification:

422100, 436180, 7386413, 7386416, 7386422, 7386424, 7386434, 7386464, 222133, 222 71

Abstract:

Devices and methods for integrated packaging, shipping, storage and precise dispensing of extremely small volumes of liquids such as aqueous solutions and compounds dissolved in organic solvents are disclosed. Devices of the invention include a sealed reservoir with an integrated metering tap. The tap includes a metering tube, which is translatable between a fill position inside the reservoir and an expel position outside the reservoir. The metering tube includes: (1) a tube end closure in a lower portion of the tube, (2) a port above the tube end closure, and (3) a piston in an upper portion of the tube. The piston is movable between a down position that seals the side port and an up position above the port. Movement of the piston from the up position to the down position can displace from 10 nanoliters to 20 microliters, e. g. , from 20 nanoliters to 2 microliters, or 50 nanoliters to 500 nanoliters.

US Patent:

6706538, Mar 16, 2004

Filed:

Jun 12, 2000

Appl. No.:

09/591807

Inventors:

Jeffrey A. Karg - Hopkinton MA
Douglas W. Kroncke - Boston MA
Michael J. Jobin - Boston MA

Assignee:

Boston Innovation Inc. - MA

International Classification:

G01N 110

US Classification:

436180, 422100, 7386413, 7386416, 7386422, 7386424, 7386434, 7386464, 222133, 222 71

Abstract:

Devices and methods for integrated packaging, shipping, storage and precise dispensing of extremely small volumes of liquids such as aqueous solutions and compounds dissolved in organic solvents are disclosed. Devices of the invention include a sealed reservoir with an integrated metering tap. The tap includes a metering tube, which is translatable between a fill position inside the reservoir and an expel position outside the reservoir. The metering tube includes: (1) a tube end closure in a lower portion of the tube, (2) a port above the tube end closure, and (3) a piston in an upper portion of the tube. The piston is movable between a down position that seals the side port and an up position above the port. Movement of the piston from the up position to the down position can displace from 10 nanoliters to 20 microliters, e. g. , from 20 nanoliters to 2 microliters, or 50 nanoliters to 500 nanoliters.

US Patent:

7459128, Dec 2, 2008

Filed:

Feb 13, 2004

Appl. No.:

10/778776

Inventors:

Jeffrey A. Karg - Hopkinton MA, US
Douglas W. Kroncke - Boston MA, US

Assignee:

Molecular BioProducts, Inc. - San Diego CA

International Classification:

B01L 3/02

US Classification:

422100, 422 99, 222133, 7386413, 436180

Abstract:

A microfluidic dispensing tap, such as for use in screening assays, includes a dispensing tube translatable within a sealed reservoir housing. The tube has an outer surface and defines an inner cavity open at a lower end of the tube, and a metering aperture extending through a side wall of the tube between the inner cavity and the outer surface to define a known volume. The tube is movable against a seal at a lower end of the reservoir housing between a first position, in which the metering aperture is disposed below the seal, and a second position, in which the metering aperture is disposed above the seal and exposed to the reservoir for entraining a discrete dose of a sample liquid within the aperture. An injector is configured to inject a known quantity of a diluent into the inner cavity of the tube and into fluidic contact with the dose of sample liquid in the aperture, such that the dose of sample liquid diffuses into the diluent to form a discrete mixture for dispensing from an open end of the tube.

US Patent:

7592185, Sep 22, 2009

Filed:

Aug 5, 2004

Appl. No.:

10/911845

Inventors:

Jeffrey A. Karg - Hopkinton MA, US
Douglas W. Kroncke - Boston MA, US

Assignee:

Molecular BioProducts, Inc. - San Diego CA

International Classification:

G01N 1/10

US Classification:

436180, 422100, 7386332, 73864, 7386401

Abstract:

A method of metering and mixing a dose of a sample liquid with a diluent liquid includes introducing a sample liquid into a channel defined in a housing. The housing defines a pocket open to the channel and sized to both collect a metered dose of the sample liquid, and to retain the collected dose by capillary force when the channel is emptied. The sample liquid is then removed from the channel under conditions that enable retention of the collected, metered dose of the sample liquid in the pocket. Following a cleaning step, a volume of diluent liquid is introduced into the channel to induce diffusion and mixing of the diluent liquid with the dose of sample liquid to form a mixture.

US Patent:

8080218, Dec 20, 2011

Filed:

Sep 21, 2009

Appl. No.:

12/563439

Inventors:

Jeffrey A. Karg - Hopkinton MA, US
Douglas W. Kroncke - Boston MA, US

Assignee:

Molecular Bio-Products, Inc. - San Diego CA

International Classification:

B01L 3/02

US Classification:

422501, 422500, 422514, 422524, 422520, 7386332, 73864, 7386401, 7386373

Abstract:

A device and method of metering and mixing a dose of a sample liquid with a diluent liquid includes introducing a sample liquid into a channel defined in a housing. The housing defines a pocket open to the channel and sized to both collect a metered dose of the sample liquid, and to retain the collected dose by capillary force when the channel is emptied. The sample liquid is then removed from the channel under conditions that enable retention of the collected, metered dose of the sample liquid in the pocket. Following a cleaning step, a volume of diluent liquid is introduced into the channel to induce diffusion and mixing of the diluent liquid with the dose of sample liquid to form a mixture.