Dale Ando - Richmond CA, US Michael C. Holmes - Richmond CA, US Yann Jouvenot - Richmond CA, US Gary Ka Leong Lee - Richmond CA, US
Assignee:
Sangamo BioSciences, Inc. - Richmond CA
International Classification:
A01N 63/00 C12P 21/06
US Classification:
424 932, 424 9321, 435 691, 435 701
Abstract:
Disclosed herein are methods and compositions for inactivating CCR-5 genes, using zinc finger nucleases (ZFNs) comprising a zinc finger protein and a cleavage domain or cleavage half-domain. Polynucleotides encoding ZFNs, vectors comprising polynucleotides encoding ZFNs, such as adenovirus (Ad) vectors, and cells comprising polynucleotides encoding ZFNs and/or cells comprising ZFNs are also provided.
Disclosed herein are methods and compositions for inactivating CCR-5 genes, using zinc finger nucleases (ZFNs) comprising a zinc finger protein and a cleavage domain or cleavage half-domain. Polynucleotides encoding ZFNs, vectors comprising polynucleotides encoding ZFNs, such as adenovirus (Ad) vectors, and cells comprising polynucleotides encoding ZFNs and/or cells comprising ZFNs are also provided.
Disclosed herein are methods and compositions for inactivating CCR-5 genes, using zinc finger nucleases (ZFNs) comprising a zinc finger protein and a cleavage domain or cleavage half-domain. Polynucleotides encoding ZFNs, vectors comprising polynucleotides encoding ZFNs, such as adenovirus (Ad) vectors, and cells comprising polynucleotides encoding ZFNs and/or cells comprising ZFNs are also provided.
Methods And Compositions For Increased Transgene Expression
Michael C. Holmes - Oakland CA, US Gary Ka Leong Lee - San Leandro CA, US
International Classification:
A61K 45/00 C12N 5/16 A61P 37/02
US Classification:
424 9321, 435326
Abstract:
Described herein are methods of expressing nucleic acids in T cells pre-exposed to a co-stimulatory signal and then transduced with adenoviral vectors. In some embodiments, the co-stimulation is provided by anti-CD3 and anti-CD28 antibodies and the adenoviral vector is pseudotyped for T-cell entry. The invention also relates to compositions for carrying out these methods, provided as kits or pharmaceutical compositions that can be used to treat diseases including immunological conditions and hematological malignancies.
Disclosed herein are methods and compositions for inactivating CCR-5 genes, using zinc finger nucleases (ZFNs) comprising a zinc finger protein and a cleavage domain or cleavage half-domain. Polynucleotides encoding ZFNs, vectors comprising polynucleotides encoding ZFNs, such as adenovirus (Ad) vectors, and cells comprising polynucleotides encoding ZFNs and/or cells comprising ZFNs are also provided.
- Richmond CA, US Michael C. Holmes - Richmond CA, US Gary Ka Leong Lee - Richmond CA, US
International Classification:
C12N 9/22 C07K 14/47
Abstract:
Disclosed herein are methods and compositions for inactivating CCR-5 genes, using zinc finger nucleases (ZFNs) comprising a zinc finger protein and a cleavage domain or cleavage half-domain. Polynucleotides encoding ZFNs, vectors comprising polynucleotides encoding ZFNs, such as adenovirus (Ad) vectors, and cells comprising polynucleotides encoding ZFNs and/or cells comprising ZFNs are also provided.
- Richmond CA, US Michael C. Holmes - Richmond CA, US Gary Ka Leong Lee - Richmond CA, US
International Classification:
C12N 9/22 C12N 15/86
Abstract:
Disclosed herein are methods and compositions for inactivating CCR-5 genes, using zinc finger nucleases (ZFNs) comprising a zinc finger protein and a cleavage domain or cleavage half-domain. Polynucleotides encoding ZFNs, vectors comprising polynucleotides encoding ZFNs, such as adenovirus (Ad) vectors, and cells comprising polynucleotides encoding ZFNs and/or cells comprising ZFNs are also provided.
Methods And Compositions For Increased Transgene Expression
- Richmond CA, US Gary Ka Leong Lee - Richmond CA, US
International Classification:
C12N 15/86 C12N 5/0783
Abstract:
Described herein are methods of expressing nucleic acids in T cells pre-exposed to a co-stimulatory signal and then transduced with adenoviral vectors. In some embodiments, the co-stimulation is provided by anti-CD3 and anti-CD28 antibodies and the adenoviral vector is pseudotyped for T-cell entry. The invention also relates to compositions for carrying out these methods, provided as kits or pharmaceutical compositions that can be used to treat diseases including immunological conditions and hematological malignancies.
Elasticdev.io
Devops and Backend Developer
Akqa Apr 2011 - Mar 2015
Devops Engineer
Headlands Technologies Llc Jun 2010 - May 2011
Linux Administrator
Greenplum Jun 2010 - Nov 2010
Platform Engineer
Village Schools International Sep 2008 - Dec 2008
Volunteer Teacher
Education:
University of California, Davis 1991 - 1996
Bachelors, Bachelor of Science
Skills:
Computer Programming Continuous Delivery Linux Finance Performance Cloud Computing Devops High Performance Computing Automation Computing
Associate Director, It Application Support And Computer Systems Validation At Biomarin Pharmaceutical Inc
Biomarin Pharmaceutical Inc.
Associate Director, It Application Support and Computer Systems Validation at Biomarin Pharmaceutical Inc
Biomarin Pharmaceutical Inc. Mar 2015 - Mar 2017
Associate Director, Business Applications Support
Biomarin Pharmaceutical Inc. Sep 2013 - Mar 2015
Senior Manager, Computer System Validation
Biomarin Pharmaceutical Inc. Sep 2011 - Sep 2013
Manager, Computer System Validation
Biomarin Pharmaceutical Inc. Feb 2010 - Sep 2011
Supervisor, Computer System Validation
Education:
The Wharton School 2018 - 2020
Master of Business Administration, Masters
Uc San Diego
Bachelors, Bachelor of Science, Bioengineering
Skills:
Computer System Validation Validation 21 Cfr Part 11 Fda Gxp Change Control Biotechnology Capa Software Documentation Pharmaceutical Industry Gmp Biopharmaceuticals Gamp Quality Assurance Iso 13485 Sop Regulatory Submissions Project Management Quality System Gcp Lims Regulatory Requirements Technology Transfer Regulatory Affairs People Development Leadership Glp Lifesciences Life Sciences Information Technology Application Support Services Business Systems Analysis It Solutions It Service Delivery Budgeting Analytics Business Technology Business Intelligence Strategic Planning
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"The way we look at supplements and vitamins and minerals is that being healthy means beyond just not being sick. It means optimizing your physical and mental well-being, and there are plenty of studies out there that support that supplements will have a positive effect," Gary Leong told CTV News.