Nicole A Moritz

US Patent:

20140057272, Feb 27, 2014

Filed:

Aug 21, 2013

Appl. No.:

13/972888

Inventors:

Richard Winegar - Palm Bay FL, US
Jacqueline Bortzner - Melbourne FL, US
Nicole Moritz - Indialantic FL, US
Stephanie Sorrell - Palm Bay FL, US

Assignee:

MRIGlobal - Kansas City MO

International Classification:

C12N 1/20
C12Q 1/04

US Classification:

435 612, 435243, 4352531, 4352533, 435 615, 435 34

Abstract:

A method of culturing fastidious bacteria where a conditioned cell culture medium is prepared in which eukaryotic cells have been cultured, but where the medium is substantially free of the cultured cells. Fastidious bacteria are cultured in the medium. The conditioned cell culture medium can contain secreted factors from the cells. These factors promote the growth of the fastidious bacteria. The fastidious bacterial culture is maintained for a time period sufficient for the fastidious bacteria to multiply. The fastidious bacteria are analyzed using PCR, DNA sequencing or microbiology characterization.