Joshua T Wong

US Patent:

6555116, Apr 29, 2003

Filed:

Jan 27, 1999

Appl. No.:

09/238379

Inventors:

Bob B. Buchanan - Berkeley CA
Gregorio del Val - El Cerrito CA
Rosa M. Lozano - Madrid, ES
Joshua H. Wong - South San Francisco CA
Boihon C. Yee - Walnut Creek CA
Oscar L. Frick - San Francisco CA

Assignee:

Regents of the University of California - Oakland CA

International Classification:

A61K 3936

US Classification:

4242751, 4241851, 514 2, 514 12, 426541, 426542, 436 86, 436120, 436904, 435 25, 435 681, 530350, 530370, 530379

Abstract:

Thioredoxin, a small dithiol protein, is a specific reductant for allergenic proteins and particularly allergenic proteins present in pollen and animal and plant sources. All targeted proteins contain disulfide (SâS) bonds that are reduced to the sulfhydryl (SH) level by thioredoxin. The proteins are allergenically active and less digestible in the oxidized (SâS) state. When reduced (SH state), they lose their allergenicity and/or become more digestible. Thioredoxin achieved this reduction when activated (reduced) either by NADPH via NADP-thioredoxin reductase (physiological conditions) or by lipoic acid chemical reductant. Skin tests carried out with sensitized dogs showed that treatment of the pollens with reduced thioredoxin prior to injection eliminated or decreased the allergenicity of the pollen. Studies showed increased digestion of the pollen proteins by pepsin following reduction by thioredoxin. Pollen proteins that have been reduced by thioredoxin are effective and safe immunotherapeutic agents for decreasing or eliminating an animals allergic reaction that would otherwise occur upon exposure to the non-reduced pollen protein.

US Patent:

6600021, Jul 29, 2003

Filed:

Nov 23, 1999

Appl. No.:

09/448111

Inventors:

Bob B. Buchanan - Berkeley CA
Karoly Kobrehel - Montpellier, FR
Boihon C. Yee - Walnut Creek CA
Joshua H. Wong - South San Francisco CA
Rosa Lozano - Madrid, ES
Jin-an Jiao - Miami FL
Sungho Shin - Taejon, KR

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

C07K 14415

US Classification:

530372, 530350, 530300, 530375, 435210, 435189

Abstract:

Methods of reducing cystine containing animal and plant proteins, and improving dough and baked goods characteristics is provided which includes the steps of mixing dough ingredients with a thiol redox protein to form a dough and baking the dough to form a baked good. The method of the present invention preferably uses reduced thioredoxin with wheat flour which imparts a stronger dough and higher loaf volumes. Methods for reducing snake, bee and scorpion toxin proteins with a thiol redox (SH) agent and thereby inactivating the protein or detoxifying the protein in an individual are also provided. Protease inhibitors, including the, Kunitz and Bowman-Birk trypsin inhibitors of soybean, were also reduced by the NADP/thioredoxin system (NADPH, thioredoxin, and NADP-thioredoxin reductase) from either or wheat germ. When reduced by thioredoxin, the Kunitz and Bowman-Birk soybean trypsin inhibitors lose their ability to inhibit trypsin. Moreover, the reduced form of the inhibitors showed increased susceptibility to heat and proteolysis by either subtilisin or a protease preparation from germinating wheat seeds.

US Patent:

6784346, Aug 31, 2004

Filed:

Mar 29, 2000

Appl. No.:

09/538864

Inventors:

Myeong-Je Cho - Alameda CA
Peggy G. Lemaux - Moraga CA
Bob B. Buchanan - Berkeley CA
Joshua Wong - San Francisco CA
Corina Marx - Oakland CA

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

A01H 500

US Classification:

800320, 800295, 8003203, 800278, 800287

Abstract:

Compositions and methods of use are provided herein to make and use transgenic plants with value-added traits.

US Patent:

6838113, Jan 4, 2005

Filed:

Apr 22, 1999

Appl. No.:

09/296927

Inventors:

Bob B. Buchanan - Berkeley CA, US
Gregorio del Val - Saint-Aubin, CH
Rosa M. Lozano - Madrid, ES
Jin-an Jiao - Ft. Lauderdale FL, US
Joshua H. Wong - South San Francisco CA, US
Boihon C. Yee - Walnut Creek CA, US

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

A23L 129
A23L 3358

US Classification:

426656, 426541, 426549, 426574, 426615

Abstract:

Thioredoxin, a small dithiol protein, is a specific reductant for major food proteins, allergenic proteins and particularly allergenic proteins present in widely used foods from animal and plant sources. All targeted proteins contain disulfide (S—S) bonds that are reduced to the sulfhydryl (SH) level by thioredoxin. The proteins are allergenically active and less digestible in the oxidized (S—S) state. When reduced (SH state), they lose their allergenicity and/or become more digestible. Thioredoxin achieved this reduction when activated (reduced) either by NADPH via NADP-thioredoxin reductase (physiological conditions) or by dithiothreitol, a chemical reductant. Skin tests and feeding experiments carried out with sensitized dogs showed that treatment of the food with reduced thioredoxin prior to ingestion eliminated or decreased the allergenicity of the food. Studies showed increased digestion of food and food proteins by pepsin and trypsin following reduction by thioredoxin.

US Patent:

7074900, Jul 11, 2006

Filed:

Apr 23, 2003

Appl. No.:

10/422422

Inventors:

Bob B. Buchanan - Berkeley CA, US
Gregorio del Val - San Diego CA, US
Rosa M. Lozano - Madrid, ES
Joshua H. Wong - San Francisco CA, US
Boihan C. Yee - Walnut Creek CA, US
Oscar L. Frick - San Francisco CA, US

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

C07K 14/00
C07K 14/415
A61K 39/35
A61K 39/36

US Classification:

530350, 530370, 530379, 514 2, 435 25, 435 681, 4241841, 4241851, 4242751, 426656, 426541

Abstract:

Thioredoxin, a small dithiol protein, is a specific reductant for allergenic proteins and particularly allergenic proteins present in pollen and animal and plant sources. All targeted proteins contain disulfide (S—S) bonds that are reduced to the sulfhydryl (SH) level by thioredoxin. The proteins are allergenically active and less digestible in the oxidized (S—S) state. When reduced (SH state), they lose their allergenicity and/or become more digestible. Thioredoxin achieved this reduction when activated (reduced) either by NADPH via NADP-thioredoxin reductase (physiological conditions) or by lipoic acid chemical reductant. Skin tests carried out with sensitized dogs showed that treatment of the pollens with reduced thioredoxin prior to injection eliminated or decreased the allergenicity of the pollen. Studies showed increased digestion of the pollen proteins by pepsin following reduction by thioredoxin. Pollen proteins that have been reduced by thioredoxin are effective and safe immunotherapeutic agents for decreasing or eliminating an animal's allergic reaction that would otherwise occur upon exposure to the non-reduced pollen protein.

US Patent:

7179964, Feb 20, 2007

Filed:

Jul 12, 2002

Appl. No.:

10/194885

Inventors:

Myeong-Je Cho - Alameda CA, US
Joshua H. Wong - San Francisco CA, US
Peggy G. Lemaux - Moraga CA, US
Bob B. Buchanan - Berkeley CA, US

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

C12N 15/82
A01H 5/00

US Classification:

800298, 800287, 8003201, 800290, 800306, 800284

Abstract:

The present invention is directed to a transgenic plant wherein at least a part of said plant includes a recombinant nucleic acid with a promoter active in the part operably linked to a nucleic acid encoding a thioredoxin polypeptide wherein the promoter is a seed or grain maturation-specific promoter and the thioredoxin polypeptide includes the amino acid sequence WCGPC. The present invention is further directed to transgenic plants that overexpress thioredoxin in seed wherein the overexpression of thioredoxin h effects a significant increase in the reduction of proteins (—SH as compared to S—S) of the albumin fraction of the seed.

US Patent:

20030215542, Nov 20, 2003

Filed:

Jun 17, 2003

Appl. No.:

10/464793

Inventors:

Bob Buchanan - Berkeley CA, US
Boihon Yee - Walnut Creek CA, US
Joshua Wong - San Francisco CA, US
Rosa Lozano - Madrid, ES
Karoly Kobrehel - Montpellier, FR
Jin-An Jiao - Miami FL, US
Sungho Shin - Taejon, KR

International Classification:

A23L001/00
C07H021/04
A61K038/44
C12N009/02
C12N001/18
C12N015/74

US Classification:

426/001000, 435/069100, 435/189000, 435/254210, 435/320100, 424/094400, 536/023200

Abstract:

Methods of reducing cystine containing animal and plant proteins, and improving dough and baked goods' characteristics is provided which includes the steps of mixing dough ingredients with a thiol redox protein to form a dough and baking the dough to form a baked good. The method of the present invention preferably uses reduced thioredoxin with wheat flour which imparts a stronger dough and higher loaf volumes. Methods for reducing snake, bee and scorpion toxin proteins with a thiol redox (SH) agent and thereby inactivating the protein or detoxifying the protein in an individual are also provided. Protease inhibitors, including the Kunitz and Bowman-Birk trypsin inhibitors of soybean, were also reduced by the NADP/thioredoxin system (NADPH, thioredoxin, and NADP-thioredoxin reductase) from either or wheat germ. When reduced by thioredoxin, the Kunitz and Bowman-Birk soybean trypsin inhibitors lose their ability to inhibit trypsin. Moreover, the reduced form of the inhibitors showed increased susceptibility to heat and proteolysis by either subtilisin or a protease preparation from germinating wheat seeds. The 2S albumin of castor seed endosperm was reduced by thioredoxin from either wheat germ or . Thioredoxin was reduced by either NADPH and NADP-thioredoxin reductase or dithiothreitol. Analyses showed that thioredoxin actively reduced the intramolecular disulfides of the 2S large subunit, but was ineffective in reducing the intermolecular disulfides that connect the large to the small subunit. A novel cystine containing protein that inhibits pullulanase was isolated. The protein was reduced by thioredoxin and upon reduction its inhibitory activity was destroyed or greatly reduced.

US Patent:

20050015831, Jan 20, 2005

Filed:

Mar 25, 2004

Appl. No.:

10/810160

Inventors:

Myeong-Je Cho - Alameda CA, US
Peggy Lemaux - Moraga CA, US
Bob Buchanan - Oakland CA, US
Joshua Wong - San Francisco CA, US
Corina Marx - Oakland CA, US

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

A01H001/00
C12N015/82

US Classification:

800278000

Abstract:

Compositions and methods of use are provided herein to make and use transgenic plants with value-added traits.

Address:

700 Kipling Ct, El Sobrante, CA 94803

Phone:

408-623-9159

VIN:

JH4CL96947C007037

Make:

ACURA

Model:

TSX

Year:

2007