Dominic W Wong

US Patent:

20080102497, May 1, 2008

Filed:

Jan 3, 2007

Appl. No.:

11/619314

Inventors:

Dominic Wong - El Cerrito CA, US
Arun Kumar Uppalanchi - Foxboro MA, US
Xiangdong Gan - North Attleboro MA, US
Bin Zhong - Cumberland RI, US

International Classification:

C12P 19/04
C12P 19/02
C12N 9/32

US Classification:

435101, 435204, 435105

Abstract:

Provided herein are methods of increasing the enzymatic rate of hydrolysis of starch substrates. In certain embodiments the method comprises contacting a starch substrate with one or more amylase family enzymes in the presence of greater than 0.001 mM manganese ions. In certain embodiments, the method comprises contacting a starch substrate with one or more glucoamylase and/or β-amylase enzymes in the presence of greater than 0.001 mM calcium ions (Ca). In certain embodiments, the method comprises contacting the starch substrate with a β-amylase in the presence of greater than 0.001 mM manganese ion, calcium ion, magnesium ion (Mg), strontium ion (Sr), barium ion (Ba) or any combination of said metal ions. In certain embodiments the method comprises contacting the starch substrate with a glucoamylase in the presence of greater than 0.001 mM manganese ion, calcium ion, lithium ion (Li), potassium ion (K), or any combination of said metal ions. Also provided herein are compositions and kits for hydrolyzing starch.