Vincent A Pieribone

US Patent:

20060018833, Jan 26, 2006

Filed:

Apr 7, 2005

Appl. No.:

11/101151

Inventors:

Randall Murphy - Glenmoor PA, US
Vincent Pieribone - New Haven CT, US

International Classification:

A61K 49/00

US Classification:

424009200, 800003000

Abstract:

Screening methods and instrumentation for candidate pharmacological agents are applied to discover compounds with muscular and/or neurological activity. The method comprises the use of teleost fish, such as the medaka (), which may be stimulated with chemical agents or an electric field to produce, for example, seizure activity and/or convulsive activity. The convulsive behavior may be recorded optically and electrically. Antagonism of the convulsive behavior is produced by application of candidate pharmacological agents to the well containing the fish. The method may include stimulation and antagonism in a plurality of sample wells with a repetitive or simultaneous application of threshold electric fields. The methods and instrumentation can be applied to the study of other serious neurological diseases such as neuropathic pain. In addition the process of assaying the protection of animals to convulsant agents the assay measures pharmacological safety parameters including sedation and cognitive impairment.

US Patent:

20060063202, Mar 23, 2006

Filed:

Aug 11, 2005

Appl. No.:

11/201575

Inventors:

Vincent Pieribone - New Haven CT, US

International Classification:

C40B 40/10
G01N 33/53
C12M 1/34

US Classification:

435007100, 435287200

Abstract:

Methods and systems of compound screening are provided. Screening methods and instrumentation for candidate pharmacological agents are applied to discover compounds with particular activity against epilepsy. The method employs teleost fish, such as the medaka (), which are stimulated with a threshold electric field to produce convulsive behavior. The convulsive behavior is recorded optically and electrically. Antagonism of the convulsive behavior is produced by application of candidate pharmacological agents to the well containing the fish. The method can include stimulation and antagonism in a plurality of sample wells with a repetitive or simultaneous application of threshold electric fields. The methods and instrumentation can be applied to the study of other serious neurological diseases such as neuropathic pain.

US Patent:

20110039278, Feb 17, 2011

Filed:

Aug 9, 2010

Appl. No.:

12/852761

Inventors:

Vincent Pieribone - New Haven CT, US

Assignee:

AFFINIMARK TECHNOLOGIES, INC., - Ellington CT

International Classification:

G01N 33/566
G01N 33/573
C12M 1/34

US Classification:

435 721, 435 74, 4352872, 435 72, 436501, 422 69

Abstract:

The present disclosure relates to detection of the presence or absence of cerebrospinal fluid (CSF) in a sample by the detection of one or more antigens that are enriched in CSF compared to their levels in other bodily fluids. The devices and methods are suitable for the detection of the presence or absence of cerebrospinal fluid in samples of mixed bodily fluids from a wide variety of human populations crossing ethnicity, age, gender, health status and genetic variability.

US Patent:

20120252698, Oct 4, 2012

Filed:

Sep 16, 2010

Appl. No.:

13/496574

Inventors:

David Gruber - Fair Lawn NJ, US
Vincent Pieribone - New Haven CT, US

International Classification:

G01N 21/64
C12N 15/63
C12N 1/21
C07K 19/00
C12N 5/10
C07K 14/00
C12N 15/11
C40B 30/08
C12N 1/15

US Classification:

506 11, 435 15, 435 21, 4353201, 4352523, 43525411, 435419, 435349, 435348, 435325, 530350, 536 231

Abstract:

The present invention concerns novel isolated fluorescent proteins, variants thereof, and polynucleotides encoding the same. Methods for making and using the polypeptides and polynucleotides are also provided. For example, methods to detect protein-protein interactions, to develop novel fluorescent reagents, to monitor cellular events, as well as cell-based methods for screening for kinase or phosphatase inhibitors, are set forth. Kits to carry out the methods of the invention are also taught.

US Patent:

20130210669, Aug 15, 2013

Filed:

Apr 17, 2013

Appl. No.:

13/864616

Inventors:

Vincent Pieribone - New Haven CT, US

Assignee:

AFFINIMARK TECHNOLOGIES, INC., - Ellington CT

International Classification:

G01N 33/68

US Classification:

506 9, 436501, 422 69

Abstract:

The present disclosure relates to detection of the presence or absence of cerebrospinal fluid (CSF) in a sample by the detection of one or more antigens that are enriched in CSF compared to their levels in other bodily fluids. The devices and methods are suitable for the detection of the presence or absence of cerebrospinal fluid in samples of mixed bodily fluids from a wide variety of human populations crossing ethnicity, age, gender, health status and genetic variability.

US Patent:

20170131296, May 11, 2017

Filed:

Nov 10, 2016

Appl. No.:

15/348563

Inventors:

- New York NY, US
- New Haven CT, US
Vincent A. Pieribone - Madison CT, US

International Classification:

G01N 33/72
G01N 33/58

Abstract:

Fluorescent proteins (Chlopsid FP I from and Chlopsid FP II from n. sp.) are used in a method for detecting bilirubin. The proteins are based on transcriptome analysis of the false moray eels, and n. sp., the later representing a heretofore undescribed species.

US Patent:

20160223571, Aug 4, 2016

Filed:

Apr 18, 2016

Appl. No.:

15/131892

Inventors:

- Ellington CT, US
Vincent Pieribone - New Haven CT, US

International Classification:

G01N 33/68

Abstract:

The present disclosure relates to detection of the presence or absence of cerebrospinal fluid (CSF) in a sample by the detection of one or more antigens that are enriched in CSF compared to their levels in other bodily fluids. The devices and methods are suitable for the detection of the presence or absence of cerebrospinal fluid in samples of mixed bodily fluids from a wide variety of human populations crossing ethnicity, age, gender, health status and genetic variability.

US Patent:

20150011018, Jan 8, 2015

Filed:

Sep 19, 2014

Appl. No.:

14/491256

Inventors:

- New Haven CT, US
Vincent Pieribone - New Haven CT, US

International Classification:

G01N 33/68

US Classification:

436501, 422 69

Abstract:

The present disclosure relates to detection of the presence or absence of cerebrospinal fluid (CSF) in a sample by the detection of one or more antigens that are enriched in CSF compared to their levels in other bodily fluids. The devices and methods are suitable for the detection of the presence or absence of cerebrospinal fluid in samples of mixed bodily fluids from a wide variety of human populations crossing ethnicity, age, gender, health status and genetic variability.