Troy A Lionberger

US Patent:

7341222, Mar 11, 2008

Filed:

Apr 7, 2005

Appl. No.:

11/100592

Inventors:

Nigel Reuel - Rio Rancho NM, US
Troy A. Lionberger - Ann Arbor MI, US
Paul C. Galambos - Albuquerque NM, US
Murat Okandan - Albuquerque NM, US
Michael S. Baker - Albuquerque NM, US

Assignee:

The United States of America as represented by the United States Department of Energy - Washington DC

International Classification:

B64C 33/00

US Classification:

244 11, 244 22, 446 35

Abstract:

A MEMS-based micro-unmanned vehicle includes at least a pair of wings having leading wing beams and trailing wing beams, at least two actuators, a leading actuator beam coupled to the leading wing beams, a trailing actuator beam coupled to the trailing wing beams, a vehicle body having a plurality of fulcrums pivotally securing the leading wing beams, the trailing wing beams, the leading actuator beam and the trailing actuator beam and having at least one anisotropically etched recess to accommodate a lever-fulcrum motion of the coupled beams, and a power source.

US Patent:

20210292751, Sep 23, 2021

Filed:

Apr 9, 2021

Appl. No.:

17/226914

Inventors:

- Emeryville CA, US
Troy A. LIONBERGER - Berkeley CA, US
Eric K. SACKMANN - Emeryville CA, US
Volker L.S. KURZ - Emeryville CA, US
Kellen C. MOBILIA - Emeryville CA, US

Assignee:

BERKELEY LIGHTS, INC. - Emeryville CA

International Classification:

C12N 15/10
B01L 3/00

Abstract:

Systems, methods, and kits therefor, enabling rapid protein evolution are described herein. A system useful in the methods described herein include a DNA synthesis component; a microfluidic system including a microfluidic device having a microfluidic channel and sequestration pens; and a computing component, which is configured to analyze assay results and, based upon the analysis, design improved DNA sequences for iterative protein evolution. The microfluidic system is configured to permit correlation of DNA sequence on a bead to its location within the microfluidic device, permit cell free protein expression of a DNA sequence captured to a bead, and to permit assay of the protein so produced.

US Patent:

20210270817, Sep 2, 2021

Filed:

Mar 11, 2021

Appl. No.:

17/198833

Inventors:

- Emeryville CA, US
Troy A. Lionberger - San Francisco CA, US
Kevin T. Chapman - Emeryville CA, US

Assignee:

Berkeley Lights, Inc. - Emeryville CA

International Classification:

G01N 33/53
B01L 3/00

Abstract:

Disclosed herein is a method for assaying binding affinity between a first molecule and a second molecule in a micro-fluidic device.

US Patent:

20210102150, Apr 8, 2021

Filed:

Oct 12, 2020

Appl. No.:

17/067971

Inventors:

- Emeryville CA, US
Troy A. Lionberger - Berkeley CA, US
Eric K. Sackmann - Oakland CA, US
Kai W. Szeto - Emeryville CA, US
Paul M. Lebel - San Mateo CA, US
Brandon R. Bruhn - San Francisco CA, US
Keith J. Breinlinger - San Rafael CA, US
Eric D. Hobbs - Livermore CA, US
Andrew W. McFarland - Berkeley CA, US
J. Tanner Nevill - El Cerrito CA, US
Xiaohua Wang - Albany CA, US

International Classification:

C12M 3/06
B01L 3/00
C12M 1/00
C12M 1/26

Abstract:

Apparatuses and methods are described for the use of optically driven bubble, convective and displacing fluidic flow to provide motive force in microfluidic devices. Alternative motive modalities are useful to selectively dislodge and displace micro-objects, including biological cells, from a variety of locations within the enclosure of a microfluidic device.

US Patent:

20210090252, Mar 25, 2021

Filed:

Oct 5, 2020

Appl. No.:

17/062907

Inventors:

- Emeryville CA, US
Paul M. Lundquist - San Francisco CA, US
John A. Tenney - Piedmont CA, US
Troy A. Lionberger - Berkeley CA, US

International Classification:

G06T 7/00
G01N 15/14
G01N 21/64
G01N 27/453
G06T 5/50

Abstract:

Methods are provided for the automated detection of micro-objects in a microfluidic device. In addition, methods are provided for repositioning micro-objects in a microfluidic device. In addition, methods are provided for separating micro-objects in a spatial region of the microfluidic device.

US Patent:

20200392567, Dec 17, 2020

Filed:

Jun 24, 2020

Appl. No.:

16/910682

Inventors:

- Emeryville CA, US
Ravi K. Ramenani - Livermore CA, US
Debjit Ray - Santa Clara CA, US
Thomas M. Vetterli - Berkeley CA, US
Annamaria Mocciaro - San Francisco CA, US
Magali Soumillon - Boston MA, US
Mark P. White - Orinda CA, US
Troy A. Lionberger - Berkeley CA, US
Kevin T. Chapman - Emeryville CA, US
Po-Yuan Tung - South San Francisco CA, US

Assignee:

Berkeley Lights, Inc. - Emeryville CA

International Classification:

C12Q 1/6809
B01J 19/00
B01L 3/00
C12Q 1/6806
C12Q 1/6841

Abstract:

Disclosed herein are methods for performing assays, including general functional assays, on a biological cell. The methods can include contacting a biological cell with a test agent for a period of time; lysing the biological cell while the biological cell is disposed within a sequestration pen located within an enclosure of a microfluidic device; and allowing RNA molecules released from the lysed biological cell to be captured by capture oligonucleotides linked to a capture object disposed within the sequestration pen of the microfluidic device. Each capture oligonucleotide can include a priming sequence that binds a primer, and a capture sequence. Each cDNA transcribed from a captured RNA can have an oligonucleotide sequence complementary to the captured RNA molecule, with the complementary oligonucleotide sequence being covalently linked to one of the capture oligonucleotides of the capture object.

US Patent:

20190172196, Jun 6, 2019

Filed:

Jun 5, 2018

Appl. No.:

16/000385

Inventors:

- Emeryville CA, US
Paul M. Lundquist - San Francisco CA, US
John A. Tenney - Piedmont CA, US
Troy A. Lionberger - Berkeley CA, US

International Classification:

G06T 7/00
G01N 21/64
G01N 15/14
G06T 5/50
G01N 27/453

Abstract:

Methods are provided for the automated detection of micro-objects in a microfluidic device. In addition, methods are provided for repositioning micro-objects in a microfluidic device. In addition, methods are provided for separating micro-objects in a spatial region of the microfluidic device.

US Patent:

20180298318, Oct 18, 2018

Filed:

Jun 15, 2018

Appl. No.:

16/010176

Inventors:

- Emeryville CA, US
Troy A. Lionberger - Berkeley CA, US
Erik K. Sackmann - Oakland CA, US
Kai W. Szeto - Emeryville CA, US
Paul M. Lebel - San Mateo CA, US
Brandon R. Bruhn - San Francisco CA, US
Keith J. Breinlinger - San Rafael CA, US
Eric D. Hobbs - Livermore CA, US
Andrew W. McFarland - Berkeley CA, US
J. Tanner Nevill - El Cerrito CA, US
Xiaohua Wang - Albany CA, US

International Classification:

C12M 3/06
B01L 3/00
C12M 1/00
C12M 1/26

Abstract:

Apparatuses and methods are described for the use of optically driven bubble, convective and displacing fluidic flow to provide motive force in microfluidic devices. Alternative motive modalities are useful to selectively dislodge and displace micro-objects, including biological cells, from a variety of locations within the enclosure of a microfluidic device.