James Boyce - Kirkland WA, US Michael Brown - Kenmore WA, US Jeffrey Fitzner - Seattle WA, US Thomas Kowski - Seattle WA, US
International Classification:
C12Q 1/48 C07F 5/02
US Classification:
435015000, 544229000
Abstract:
Various embodiments of the present invention are related to kinase-directed, activity-based probes (“KABPs”) that bind to, and label, kinases. Each KABP includes a binding group that is recognized and bound by one or more kinases, a reactive group that tightly, and generally irreversibly, binds to the kinase, a tag group that provides a detectable label for the kinase-KABP pair, or that serves as a chemical handle for subsequent procedures and processes, and a linker group that links the tag group to one or more of the reactive group and the binding group, spacing the tag group from the reactive and binding groups. Additional embodiments of the present invention are directed to methods for identifying kinases within, and isolating kinases from, living cells by use of one or more KABPs.
James Boyce - Kirkland WA, US Michael Brown - Kenmore WA, US Jeffrey Fitzner - Seattle WA, US Thomas Kowski - Seattle WA, US
International Classification:
C12Q 1/48 C07D 241/36 C07F 5/02
US Classification:
435015000, 544353000, 544229000
Abstract:
Various embodiments of the present invention are directed to kinase-directed, activity-based probes (“KABPs”) that tightly bind to, and label, kinases. Each KABP includes a binding group that is recognized and bound by one or more kinases, a reactive group that tightly, and generally irreversibly, binds to the kinase, a tag group that labels the kinase, or that serves a chemical handle for subsequent procedures and processes, and a linker group that links the tag group to one or more of the reactive group and the binding group. Additional embodiments of the present invention are directed to methods for identifying kinases within, and isolating kinases from, living cells by use of one or more KABPs.
Alkermes
Research Fellow
Alkermes Dec 2018 - Nov 2019
Associate Director
Hikma Pharmaceuticals Aug 2017 - Nov 2019
Manager
Juno Therapeutics, Inc. May 2015 - Jul 2017
Associate Director
Amgen Aug 2005 - May 2015
Principal Scientist
Education:
Bowling Green State University 1991 - 1997
Master of Science, Masters, Bachelors, Bachelor of Science, Chemistry
Bowling Green State University 1997
Master of Science, Masters
Skills:
Assay Development Biotechnology Biopharmaceuticals Elisa High Throughput Screening Protein Chemistry Monoclonal Antibodies Drug Discovery Antibodies Biochemistry Cell Culture Technology Transfer Chromatography Western Blotting Lims Purification Protein Purification Immunoassays In Vitro Immunology Drug Development Formulation Ind Design Thinking Mass Spectrometry Sds Page Inflammation Protein Engineering Bioanalysis Lifesciences Laboratory Automation Tissue Culture Small Molecules Mammalian Cell Culture Protein Expression Eln Design Think Organizational Leadership Enzyme Assays Qpcr Sop Signal Transduction Immunoprecipitation Gel Electrophoresis Downstream Processing Column Chromatography Bioinformatics Proteomics Drug Design Automation