Group Health Bellevue Medical Center Otolaryngology 11511 NE 10 St STE 2A, Bellevue, WA 98004 425-502-3770 (phone), 425-502-3771 (fax)
Education:
Medical School University of California, Los Angeles David Geffen School of Medicine Graduated: 1980
Procedures:
Myringotomy and Tympanotomy Rhinoplasty Sinus Surgery Tonsillectomy or Adenoidectomy Tracheostomy Tympanoplasty
Conditions:
Acute Upper Respiratory Tract Infections Chronic Sinusitis Deviated Nasal Septum Hearing Loss Otitis Media
Languages:
English Spanish
Description:
Dr. Short graduated from the University of California, Los Angeles David Geffen School of Medicine in 1980. He works in Bellevue, WA and specializes in Otolaryngology.
Dr. Short graduated from the A.T. Still University of Health Sciences/ Kirksville College of Osteopathic Medicine in 1983. He works in Marysville, KS and 1 other location and specializes in Pulmonary Critical Care Medicine and Pulmonary Disease. Dr. Short is affiliated with Community Memorial Healthcare, Manhattan Surgical Hospital and Via Christi Hospital Manhattan.
Igor Y. Khandros - Orinda CA, US Gaetan L. Mathieu - Varennes, CA Steven W. Short - Pleasanton CA, US Ming C. Wu - Moraga CA, US
International Classification:
C12N 13/00
US Classification:
435450, 4351731, 4352831, 4351736
Abstract:
Two or more biological micro-objects can be grouped in a liquid medium in a chamber. Grouping can comprise bringing into and holding in proximity or contact the micro-objects in a group, breaching the membrane of one or more of the micro-objects in a group, subjecting one or more of the micro-objects in a group to electroporation, and/or tethering to each other the micro-objects in a group. The micro-objects in the group can then be combined into a single biological object.
Micro-Fluidic Devices For Assaying Biological Activity
- Emeryville CA, US Daniele Malleo - El Cerrito CA, US J. Tanner Nevill - El Cerrito CA, US Steven W. Short - Pleasanton CA, US Mark P. White - San Francisco CA, US M. Jimena Loureiro - Albany CA, US
Biological activity in holding pens in a micro-fluidic device can be assayed by placing in the holding pens capture objects that bind a particular material of interest produced by the biological activity. The biological material of interest that binds to each capture object can then be assessed, either in the micro-fluidic device or after exporting the capture object from the micro-fluidic device. The assessment can be utilized to characterize the biological activity in each holding pen. The biological activity can be production of the biological material of interest. Thus, the biological activity can correspond to or arise from one or more biological cells. Biological cells within a holding pen can be clonal cell colonies. The biological activity of each clonal cell colony can be assayed while maintaining the clonal status of each colony.
Microfluidic Devices Having Isolation Pens And Methods Of Testing Biological Micro-Objects With Same
- Emeryville CA, US Eric D. Hobbs - Emeryville CA, US J. Tanner Nevill - El Cerrito CA, US Daniele Malleo - El Cerrito CA, US Steven W. Short - Pleasanton CA, US
International Classification:
B01L 3/00 G01N 33/558
Abstract:
A microfluidic device can comprise at least one swept region that is fluidically connected to unswept regions. The fluidic connections between the swept region and the unswept regions can enable diffusion but substantially no flow of media between the swept region and the unswept regions. The capability of biological micro-objects to produce an analyte of interest can be assayed in such a microfluidic device. Biological micro-objects in sample material loaded into a microfluidic device can be selected for particular characteristics and disposed into unswept regions. The sample material can then be flowed out of the swept region and an assay material flowed into the swept region. Flows of medium in the swept region do not substantially affect the biological micro-objects in the unswept regions, but any analyte of interest produced by a biological micro-object can diffuse from an unswept region into the swept region, where the analyte can react with the assay material to produce a localized detectable reaction. Any such detected reactions can be analyzed to determine which, if any, of the biological micro-objects are producers of the analyte of interest.
Micro-Fluidic Devices For Assaying Biological Activity
- Emeryville CA, US Daniele Malleo - El Cerrito CA, US J. Tanner Nevill - El Cerrito CA, US Steven W. Short - Pleasanton CA, US Mark P. White - San Francisco CA, US M. Jimena Loureiro - Albany CA, US
Biological activity in holding pens in a micro-fluidic device can be assayed by placing in the holding pens capture objects that bind a particular material of interest produced by the biological activity. The biological material of interest that binds to each capture object can then be assessed, either in the micro-fluidic device or after exporting the capture object from the micro-fluidic device. The assessment can be utilized to characterize the biological activity in each holding pen. The biological activity can be production of the biological material of interest. Thus, the biological activity can correspond to or arise from one or more biological cells. Biological cells within a holding pen can be clonal cell colonies. The biological activity of each clonal cell colony can be assayed while maintaining the clonal status of each colony.
Micro-Fluidic Devices For Assaying Biological Activity
- Emeryville CA, US Daniele Malleo - El Cerrito CA, US J. Tanner Nevill - El Cerrito CA, US Steven W. Short - Pleasanton CA, US Mark P. White - San Francisco CA, US M. Jimena Loureiro - Albany CA, US
Biological activity in holding pens in a micro-fluidic device can be assayed by placing in the holding pens capture objects that bind a particular material of interest produced by the biological activity. The biological material of interest that binds to each capture object can then be assessed, either in the micro-fluidic device or after exporting the capture object from the micro-fluidic device. The assessment can be utilized to characterize the biological activity in each holding pen. The biological activity can be production of the biological material of interest. Thus, the biological activity can correspond to or arise from one or more biological cells. Biological cells within a holding pen can be clonal cell colonies. The biological activity of each clonal cell colony can be assayed while maintaining the clonal status of each colony.
Providing Dep Manipulation Devices And Controllable Electrowetting Devices In The Same Microfluidic Apparatus
- Emeryville CA, US Daniele Malleo - El Cerrito CA, US J. Tanner Nevill - El Cerrito CA, US Steven W. Short - Pleasanton CA, US Ming C. Wu - Moraga CA, US
International Classification:
B01L 3/00 B03C 5/02 B03C 5/00
Abstract:
A structure for providing a boundary for a chamber in a microfluidic apparatus can comprise dielectrophoresis (DEP) configurations each having an outer surface and electrowetting (EW) configurations each having an electrowetting surface. The DEP configurations can facilitate generating net DEP forces with respect to the outer surfaces of the DEP configurations to move micro-objects on the outer surfaces, and the EW configurations can facilitate changing wetting properties of the electrowetting surfaces to move droplets of liquid medium on the electrowetting surfaces.
Dep Force Control And Electrowetting Control In Different Sections Of The Same Microfluidic Apparatus
- Emeryville UT, US J. Tanner NEVILL - El Cerrito CA, US Steven W. SHORT - Pleasanton CA, US Ming C. WU - Moraga CA, US
International Classification:
B01L 3/00 B03C 5/02 B03C 5/00 C12M 3/06 C12M 1/12
Abstract:
A microfluidic apparatus can comprise a dielectrophoresis (DEP) configured section for holding a first liquid medium and selectively inducing net DEP forces in the first liquid medium. The microfluidic apparatus can also comprise an electrowetting (EW) configured section for holding a second liquid medium on an electrowetting surface and selectively changing an effective wetting property of the electrowetting surface. The DEP configured section can be utilized to select and move a micro-object in the first liquid medium. The EW configured section can be utilized to pull a droplet of the first liquid medium into the second liquid medium.
Dep Force Control And Electrowetting Control In Different Sections Of The Same Microfluidic Apparatus
- Emeryville CA, US J. Tanner Nevill - El Cerrito CA, US Steven W. Short - Pleasanton CA, US Ming C. Wu - Moraga CA, US
Assignee:
Berkeley Lights, Inc. - Emeryville CA
International Classification:
B01L 3/00
Abstract:
A microfluidic apparatus can comprise a dielectrophoresis (DEP) configured section for holding a first liquid medium and selectively inducing net DEP forces in the first liquid medium. The microfluidic apparatus can also comprise an electrowetting (EW) configured section for holding a second liquid medium on an electrowetting surface and selectively changing a wetting property of the electrowetting surface. The DEP configured section can be utilized to select and move a micro-object in the first liquid medium. The EW configured section can be utilized to pull a droplet of the first liquid medium into the second liquid medium.
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