Group Health Bellevue Medical Center Otolaryngology 11511 NE 10 St STE 2A, Bellevue, WA 98004 425-502-3770 (phone), 425-502-3771 (fax)
Education:
Medical School University of California, Los Angeles David Geffen School of Medicine Graduated: 1980
Procedures:
Myringotomy and Tympanotomy Rhinoplasty Sinus Surgery Tonsillectomy or Adenoidectomy Tracheostomy Tympanoplasty
Conditions:
Acute Upper Respiratory Tract Infections Chronic Sinusitis Deviated Nasal Septum Hearing Loss Otitis Media
Languages:
English Spanish
Description:
Dr. Short graduated from the University of California, Los Angeles David Geffen School of Medicine in 1980. He works in Bellevue, WA and specializes in Otolaryngology.
Dr. Short graduated from the A.T. Still University of Health Sciences/ Kirksville College of Osteopathic Medicine in 1983. He works in Marysville, KS and 1 other location and specializes in Pulmonary Critical Care Medicine and Pulmonary Disease. Dr. Short is affiliated with Community Memorial Healthcare, Manhattan Surgical Hospital and Via Christi Hospital Manhattan.
Igor Y. Khandros - Orinda CA, US Gaetan L. Mathieu - Varennes, CA Steven W. Short - Pleasanton CA, US Ming C. Wu - Moraga CA, US
International Classification:
C12N 13/00
US Classification:
435450, 4351731, 4352831, 4351736
Abstract:
Two or more biological micro-objects can be grouped in a liquid medium in a chamber. Grouping can comprise bringing into and holding in proximity or contact the micro-objects in a group, breaching the membrane of one or more of the micro-objects in a group, subjecting one or more of the micro-objects in a group to electroporation, and/or tethering to each other the micro-objects in a group. The micro-objects in the group can then be combined into a single biological object.
- Pleasanton CA, US Anthony MAKAREWICZ - Livermore CA, US Steven SHORT - Pleasanton CA, US
International Classification:
B01L 3/00 G01N 15/10 G01N 15/14
Abstract:
Methods and systems for sorting particles are provided. Methods and systems for sorting cell beads are provided. In some cases, cell beads may be sorted from particles unoccupied with cell derivatives. In some cases, singularly occupied cell beads may be sorted from unoccupied particles and multiply occupied cell beads.
Micro-Fluidic Devices For Assaying Biological Activity
- Emeryville CA, US Daniele Malleo - El Cerrito CA, US J. Tanner Nevill - El Cerrito CA, US Steven W. Short - Pleasanton CA, US Mark P. White - San Francisco CA, US M. Jimena Loureiro - Albany CA, US
Biological activity in holding pens in a micro-fluidic device can be assayed by placing in the holding pens capture objects that bind a particular material of interest produced by the biological activity. The biological material of interest that binds to each capture object can then be assessed, either in the micro-fluidic device or after exporting the capture object from the micro-fluidic device. The assessment can be utilized to characterize the biological activity in each holding pen. The biological activity can be production of the biological material of interest. Thus, the biological activity can correspond to or arise from one or more biological cells. Biological cells within a holding pen can be clonal cell colonies. The biological activity of each clonal cell colony can be assayed while maintaining the clonal status of each colony.
Capturing Specific Nucleic Acid Materials From Individual Biological Cells In A Micro-Fluidic Device
- Emeryville CA, US Eric D. Hobbs - Livermore CA, US Steven W. Short - Pleasanton CA, US Mark P. White - San Francisco CA, US Daniele Malleo - San Jose CA, US
International Classification:
B03C 5/00 B03C 5/02 B03C 11/00 B01L 3/00
Abstract:
Individual biological cells can be selected in a micro-fluidic device and moved into isolation pens in the device. The cells can then be lysed in the pens, releasing nucleic acid material, which can be captured by one or more capture objects in the pens. The capture objects with the captured nucleic acid material can then be removed from the pens. The capture objects can include unique identifiers, allowing each capture object to be correlated to the individual cell from which the nucleic acid material captured by the object originated.
- Pleasanton CA, US Denis PRISTINSKI - Dublin CA, US Preyas SHAH - Milpitas CA, US Steven William SHORT - Pleasanton CA, US Dieter WILK - San Jose CA, US Siyuan XING - Newark CA, US
International Classification:
C12Q 1/6874 C12N 15/10
Abstract:
Provided in some aspects are methods for light-controlled in situ surface patterning of a substrate. Compositions such as nucleic acid arrays produced by the methods are also disclosed. In some embodiments, a method disclosed herein comprises using photoresist for photocontrollable hybridization and/or ligation of nucleic acid molecules, wherein photoresist removal allows hybridization and/or ligation of nucleic acid molecules at the exposed area. A large diversity of barcodes can be created in molecules on the substrate via sequential rounds of light exposure, hybridization, and ligation.
Microfluidic Devices Having Isolation Pens And Methods Of Testing Biological Micro-Objects With Same
- Emeryville CA, US Eric D. Hobbs - Emeryville CA, US J. Tanner Nevill - El Cerrito CA, US Daniele Malleo - El Cerrito CA, US Steven W. Short - Pleasanton CA, US
International Classification:
B01L 3/00 G01N 33/558
Abstract:
A microfluidic device can comprise at least one swept region that is fluidically connected to unswept regions. The fluidic connections between the swept region and the unswept regions can enable diffusion but substantially no flow of media between the swept region and the unswept regions. The capability of biological micro-objects to produce an analyte of interest can be assayed in such a microfluidic device. Biological micro-objects in sample material loaded into a microfluidic device can be selected for particular characteristics and disposed into unswept regions. The sample material can then be flowed out of the swept region and an assay material flowed into the swept region. Flows of medium in the swept region do not substantially affect the biological micro-objects in the unswept regions, but any analyte of interest produced by a biological micro-object can diffuse from an unswept region into the swept region, where the analyte can react with the assay material to produce a localized detectable reaction. Any such detected reactions can be analyzed to determine which, if any, of the biological micro-objects are producers of the analyte of interest.
Methods And Systems For Sorting Droplets And Beads
Methods and systems for sorting droplets are provided. In some cases, occupied droplets may be sorted from unoccupied droplets. In some cases, singularly occupied droplets may be sorted from unoccupied droplets and multiply occupied droplets. Methods and systems for sorting cell beads are provided. In some cases, cell beads may be sorted from particles unoccupied with cell derivatives. In some cases, singularly occupied cell beads may be sorted from unoccupied particles and multiply occupied cell beads. Methods and systems for selectively polymerizing droplets based on occupancy and size of the droplets are provided.
Micro-Fluidic Devices For Assaying Biological Activity
- Emeryville CA, US Daniele Malleo - El Cerrito CA, US J. Tanner Nevill - El Cerrito CA, US Steven W. Short - Pleasanton CA, US Mark P. White - San Francisco CA, US M. Jimena Loureiro - Albany CA, US
Biological activity in holding pens in a micro-fluidic device can be assayed by placing in the holding pens capture objects that bind a particular material of interest produced by the biological activity. The biological material of interest that binds to each capture object can then be assessed, either in the micro-fluidic device or after exporting the capture object from the micro-fluidic device. The assessment can be utilized to characterize the biological activity in each holding pen. The biological activity can be production of the biological material of interest. Thus, the biological activity can correspond to or arise from one or more biological cells. Biological cells within a holding pen can be clonal cell colonies. The biological activity of each clonal cell colony can be assayed while maintaining the clonal status of each colony.
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