Shawn R Feaster

US Patent:

6642037, Nov 4, 2003

Filed:

Apr 26, 2000

Appl. No.:

09/558512

Inventors:

Richard K. Gordon - Potomac MD
Bhupendra P. Doctor - Potomac MD
Ashima Saxena - Fairfax VA
Shawn R. Feaster - Damascus MD
Donald Maxwell - Baltimore MD
Michelle Ross - Edgewood MD
David Lenz - Bel Air MD
Keith Lejeune - Pittsburgh PA
Alan Russell - Wexford PA

Assignee:

The United States of America as represented by the Secretary of the Army - Washington DC

International Classification:

C12N 1104

US Classification:

435182, 435180, 4352625

Abstract:

An enzymatically active reusable sponge or foam, capable of regeneration with oximes, made of a polymer such as polyurethane is prepared for detoxification of hazardous compounds such as organophosphorus and organosulfur compounds. The foam or sponge contains a plurality of enzymes including enzymes selected from acetylcholinesterase, butyrylcholinesterase, triesterase, pseudocholinesterase, organophosphate hydrase, phosphotriesterase, paraoxonase and organophosphorus and organosulfur hydrolyzing enzymes. The sponge or foam may additionally contain activated carbon and an enzyme reactivation compound. A kit can be formed containing the sponge or foam and the compound for enzyme reactivation. The enzymatically active foam or sponge may be prepared using a two chamber device where enzymes and prepolymer are passed from separate chambers into a static mixing stator and are subjected to low shear mixing and extrusion to form by the sponge or foam.

US Patent:

6746850, Jun 8, 2004

Filed:

May 4, 2001

Appl. No.:

09/848370

Inventors:

Shawn R. Feaster - Damascus MD
Richard K. Gordon - Potomac MD
Bhupendra P. Doctor - Potomac MD

Assignee:

The United States of America as represented by the Secretary of the Army - Washington DC

International Classification:

C12Q 146

US Classification:

435 20

Abstract:

An assay for detecting, measuring, or monitoring the activity or concentration of at least two proteins that have similar or overlapping properties is disclosed. The assay comprises first determining the sensitivity coefficients of the substrates for each of the proteins in which the concentrations are to be determined. This method may be used for detecting, measuring, or monitoring the activity and concentration of AChE, BChE, or both in a test sample which test sample may be whole and unprocessed blood or tissue. Also disclosed are methods of using the assay to detect a subjects exposure to an agent which affects cholinesterase, determine the efficacy or progress of a treatment, determine the amount of protection provided against exposure to an agent which affects cholinesterase, or both, screen a subject for having a drug sensitivity or a particular disease, detect a change in red blood cell count of a subject, determine whether a candidate compound affects cholinesterase. Also disclosed are devices and kits for detecting, measuring, or monitoring the activities and concentrations of AChE, BChE, or both.

US Patent:

7279136, Oct 9, 2007

Filed:

Dec 13, 2005

Appl. No.:

11/301631

Inventors:

James M. Takeuchi - Roswell GA, US
Xuedong Song - Roswell GA, US
Kaiyuan Yang - Cumming GA, US
Ning Wei - Roswell GA, US
Shawn R. Feaster - Duluth GA, US

International Classification:

G01N 21/00
G01N 31/22
G01N 15/06
G01N 33/53
G01N 33/566
G01N 31/00
B01L 3/00
B32B 5/02
C12Q 1/00
C12M 1/36
C12M 1/34
C12M 3/00

US Classification:

422100, 422 55, 422 56, 422 57, 422 61, 422 681, 422101, 435 4, 435 71, 4352864, 4352865, 4352871, 4352872, 4352877, 4352879, 4352885, 4352887, 436501, 436518, 436 17, 436 18, 436825

Abstract:

A diagnostic method and associated test kit for detecting an analyte residing in a test sample is provided. A sample membrane is utilized having a collection region and a detection region, the collection region having a known saturation volume for the intended test sample. A barrier is defined between the collection region and the detection region. The collection region is saturated with the test sample having a volume of less than about 100 microliters so that a known volume of the test sample is contained in the collection region. The barrier is removed from between the collection region and detection region of the membrane and a diluent is supplied to the collection region of the membrane to facilitate flow of the test sample from the collection region to the detection region of the membrane.

US Patent:

7314763, Jan 1, 2008

Filed:

Aug 27, 2002

Appl. No.:

10/228838

Inventors:

Xuedong Song - Roswell GA, US
Rosann Kaylor - Cumming GA, US
Shawn Feaster - Duluth GA, US
Kaiyuan Yang - Cumming GA, US

Assignee:

Kimberly-Clark Worldwide, Inc. - Neenah WI

International Classification:

G01N 33/553
G01N 31/00
G01N 33/31
G01N 33/53

US Classification:

436526, 436518, 436 8, 436546, 436538, 436166, 435969, 435970

Abstract:

A fluidics-based assay device for detecting the presence or quantity of an analyte residing in a test sample is provided. The device utilizes a self-calibrated magnetic binding assay format (e. g. , sandwich, competitive, etc. ) that includes detection probes capable of generating a detection signal (e. g. , fluorescent non-magnetic particles) and calibration probes capable of generating a calibration signal (e. g. , fluorescent magnetic particles). The amount of the analyte within the test sample is proportional (e. g. , directly or inversely) to the intensity of the detection signal calibrated by the intensity of the calibration signal. It has been discovered that the fluidics-based device of the present invention provides an accurate, inexpensive, and readily controllable method of determining the presence of an analyte in a test sample.

US Patent:

7390674, Jun 24, 2008

Filed:

Mar 14, 2005

Appl. No.:

11/079730

Inventors:

Shawn Ray Feaster - Duluth GA, US
Kaiyuan Yang - Cumming GA, US

Assignee:

Kimberly-Clark Worldwide, Inc. - Neenah WI

International Classification:

G01N 33/53

US Classification:

436514, 4352871, 4352872, 4352877, 4352879, 435970, 435805, 435810, 436518, 436164, 436169, 436524, 436529, 436805, 436810, 422 56, 422 60

Abstract:

There is provided a lateral flow assay device for detecting the presence or quantity of an analyte residing in a test sample where the lateral flow assay device has a porous membrane in communication with a wicking pad. The porous membrane has a detection zone which has a chromophore configured to chemically react with an analyte or a secondary trigger or a reaction product from the analyte and a trigger generating reagent(s), to generate a visually detectible signal. Additional chrmophore zones may be located downstream from the first chrmophore zone to generate signals of varying color. Scavenging zones may be included between chromophore zones to attenuate the signal by reacting with the analyte without generating a visually detectable signal.

US Patent:

7552501, Jun 30, 2009

Filed:

Apr 29, 2005

Appl. No.:

11/118078

Inventors:

Kaiyuan Yang - Cumming GA, US
Jeffrey E. Fish - Dacula GA, US
Shawn R. Feaster - Duluth GA, US

Assignee:

Kimberly-Clark Worldwide, Inc. - Neenah WI

International Classification:

A41D 13/08
A47L 13/18

US Classification:

15227, 2 21, 2163

Abstract:

A finger cover, such as a finger wipe that can fit onto a human finger, is provided with an improved seam structure. The cover includes a pocket member having an open end for the insertion of a finger. The pocket member is formed by a first panel attached to a second panel along a flush outwardly facing circumferential edge seam. The seam is less than about 1 millimeter (mm) in width and about 1 mm in height. Additional reinforcing weld points may be provided at various locations along the seam.

US Patent:

7618810, Nov 17, 2009

Filed:

Dec 14, 2005

Appl. No.:

11/300650

Inventors:

Kaiyuan Yang - Cumming GA, US
Shawn R. Feaster - Duluth GA, US
Ning Wei - Roswell GA, US
Rosann M. Kaylor - Cumming GA, US
Chibueze O. Chidebelu-Eze - Atlanta GA, US

Assignee:

Kimberly-Clark Worldwide, Inc. - Neenah WI

International Classification:

G01N 21/00
G01N 15/06
G01N 33/53
G01N 31/00
G01N 33/543
B01L 3/00
B01L 3/02
B01L 11/00
C12Q 1/00
C12M 1/36
C12M 1/34
C12M 3/00

US Classification:

4352865, 422 55, 422 56, 422 57, 422 61, 422 681, 422100, 422101, 435 4, 435 71, 4352864, 4352871, 4352872, 4352877, 4352879, 4352885, 4352887, 436501, 436518, 436 17, 436 18, 436825

Abstract:

A diagnostic method and associated test kit for detecting an analyte residing in a test sample is provided. The kit includes a housing, and a membrane disposed within the housing having a detection region and a collection region. A blood sample meter is provided having a first end for absorption of a blood sample, a filtering section adjacent to the first end that filters red blood cell components from the blood sample, and a storage section adjacent to the filtering section that receives plasma or serum from the filtering section. An opening in the housing is sized for insertion of the sample meter into the housing such that the storage section of the sample meter is disposed in fluid communication with the collection region of the membrane. The plasma or serum is transferred from the storage section of the sample meter to the collection region of the membrane for subsequent migration to the detection region.

US Patent:

7682817, Mar 23, 2010

Filed:

Dec 23, 2004

Appl. No.:

11/021545

Inventors:

David Samuel Cohen - Alpharetta GA, US
Shawn Ray Feaster - Duluth GA, US

International Classification:

C12M 1/36

US Classification:

4352871, 4352865, 4352882, 4352885, 422129, 422140, 422156, 436524

Abstract:

A microfluidic assay device for determining the presence or absence of an analyte within a fluid test sample is provided. The present invention provides a technique for achieving continuous flow in a microfluidic device by using at least one input channel, an analysis zone, and a plurality of wicking channels disposed about the perimeter of the analysis zone. In one embodiment, for example, the wicking channels extend radially from the analysis zone. As a result of the particular configuration of the microfluidic device, an assay may performed in a “single step” without the need for active forces, such as a pressure source, electrokinetic force, etc. , to induce flow of the fluid test sample through the device. Likewise, flow rate is controlled so that the dwell time of the fluid test sample within the analysis zone is long enough to allow for the desired reactions and/or detection.