Sai Prashant Reddy

US Patent:

20110312505, Dec 22, 2011

Filed:

May 17, 2011

Appl. No.:

13/109467

Inventors:

Sai Reddy - Berkeley IL, US
Xin Ge - Riverside CA, US
Jason Lavinder - Round Rock TX, US
Daniel Boutz - Austin TX, US
Andrew D. Ellington - Austin TX, US
Edward M. Marcotte - Austin TX, US
George Georgiou - Austin TX, US

International Classification:

C40B 20/00
C12Q 1/68
C07K 16/00
G01N 33/68
C12Q 1/34
G01N 33/53

US Classification:

506 2, 435 18, 435 792, 5303871, 436 89, 435 61

Abstract:

Methods and compositions for identification of candidate antigen-specific variable regions as well as generation of antibodies or antigen-binding fragments that could have desired antigen specificity are provided. For example, in certain aspects methods for determining amino acid sequences of serum antibody CDR and abundancy level are described. In some aspects, methods for determining nucleic acid sequences of antibody variable region sequences and frequency are provided. Furthermore, the invention provides methods for identification and generation of antibody or antigen-binding fragments that comprise highly-represented CDR.