Perry J Riggle

US Patent:

62515934, Jun 26, 2001

Filed:

May 27, 1998

Appl. No.:

9/084346

Inventors:

Yigal Koltin - Newton MA
Perry Riggle - Norwood MA
Vicky Gavrias - Watertown MA
Chris Bulawa - Arlington MA
Kenneth R. Winter - Seattle WA

Assignee:

Millennium Pharmaceuticals, Inc. - Cambridge MA

International Classification:

C12Q 168
C12N 114
C12N 1563
C07H 2104

US Classification:

435 6

Abstract:

A polynucleotide encoding chitin synthase (CHS1), an enzyme essential for cell wall synthesis and yeast cell growth, is provided. A maltose responsive promoter (MRP) isolated using the promoter library of the invention is also described. The present invention also provides a vector for isolation of a eukaryotic regulatory polynucleotide, i. e. , promoter. The vector is useful in the method of the invention which comprises identifying a eukaryotic regulatory polynucleotide, i. e. , promoter region, by complementing the growth of an auxotrophic host cell containing the vector of the invention, which includes a promoter region operably linked to a promoterless auxotrophic gene. The vector is introduced into the host cell chromosome by targeted integration. Also provided is a library containing host cells having the vector of the invention integrated in the chromosome of the host cell.

US Patent:

60201339, Feb 1, 2000

Filed:

Jan 8, 1998

Appl. No.:

9/004225

Inventors:

Yigal Koltin - Newton MA
Perry Riggle - Norwood MA
Vicky Gavrias - Upton MA
Chris Bulawa - Arlington MA
Ken Winter - Sunshine Canyon Boulder CO

Assignee:

Millennium Pharmaceuticals, Inc. - Cambridge MA

International Classification:

C12Q 168
C12Q 148
C12N 910
A61K 3845

US Classification:

435 6

Abstract:

A polynucleotide encoding chitin synthase (CHS1), an enzyme essential for cell wall synthesis and yeast cell growth, is provided. A maltose responsive promoter (MRP) isolated using the promoter library of the invention is also described. The present invention also provides a vector for isolation of a eukaryotic regulatory polynucleotide, i. e. , promoter. The vector is useful in the method of the invention which comprises identifying a eukaryotic regulatory polynucleotide, i. e. , promoter region, by complementing the growth of an auxotrophic host cell containing the vector of the invention, which includes a promoter region operably linked to a promoterless auxotrophic gene. The vector is introduced into the host cell chromosome by targeted integration. Also provided is a library containing host cells having the vector of the invention integrated in the chromosome of the host cell.

US Patent:

58245456, Oct 20, 1998

Filed:

Nov 1, 1995

Appl. No.:

8/551437

Inventors:

Yigal Koltin - Newton MA
Perry Riggle - Norwood MA
Vicky Gavrias - Watertown MA
Chris Bulawa - Arlington MA
Ken Winter - Cambridge MA

Assignee:

Millennium Pharaceuticals, Inc. - Cambridge MA

International Classification:

C12N 1500

US Classification:

4353201

Abstract:

A polynucleotide encoding chitin synthase (CHS1), an enzyme essential for cell wall synthesis and yeast cell growth, is provided. A maltose responsive promoter (MRP) isolated using the promoter library of the invention is also described. The present invention also provides a vector for isolation of a eukaryotic regulatory polynucleotide, i. e. , promoter. The vector is useful in the method of the invention which comprises identifying a eukaryotic regulatory polynucleotide, i. e. , promoter region, by complementing the growth of an auxotrophic host cell containing the vector of the invention, which includes a promoter region operably linked to a promoterless auxotrophic gene. The vector is introduced into the host cell chromosome by targeted integration. Also provided is a library containing host cells having the vector of the invention integrated in the chromosome of the host cell.