Mohammad A Nasir

US Patent:

6482601, Nov 19, 2002

Filed:

Sep 11, 2000

Appl. No.:

09/659663

Inventors:

Mohammad Sarwar Nasir - Grayslake IL
Michael E. Jolley - Round Lake IL

Assignee:

Diachemix LLC - Milwaukee WI

International Classification:

G01N 33569

US Classification:

435 731, 435341, 435810, 435975, 436536, 436537, 436 17, 436172, 436177, 436825, 530371, 5303885, 5303891, 530823

Abstract:

A homogeneous assay for determining the fumonisin content in grains uses the technique of fluorescence polarization. A grain extract is prepared by shaking a crushed grain sample with a solvent. A mixture is prepared by combining the grain extract with a tracer and with monoclonal antibodies specific to fumonisin. The tracer is able to bind to the monoclonal antibodies to produce a detectable change in fluorescence polarization. The tracer is prepared by conjugating fumonisin to a suitable fluorophore. The fluorescence polarization of the mixture is measured. The fumonisin concentration of the mixture may be calculated using a standard curve obtained by measuring the fluorescence polarization of a series of fumonisin solutions of known concentration.

US Patent:

6812036, Nov 2, 2004

Filed:

Jul 11, 2001

Appl. No.:

09/903061

Inventors:

Mohammad Sarwar Nasir - Grayslake IL
Michael E. Jolley - Round Lake IL

Assignee:

Diachemix LLC - Milwaukee WI

International Classification:

G01N 2176

US Classification:

436172, 435 71, 435 4, 435 21, 435 79, 436528, 436543, 436518, 436546

Abstract:

A homogeneous assay for determining the deoxynivalenol (DON) content in grains uses the technique of fluorescence polarization. A grain extract is prepared by shaking a crushed grain sample with water. A mixture is prepared by combining the grain extract with a tracer and with monoclonal antibodies specific to DON. The tracer is able to bind to the monoclonal antibodies to produce a detectable change in fluorescence polarization. The tracer is prepared by conjugating DON to a suitable fluorophore. The fluorescence polarization of the mixture is measured. The DON concentration of the mixture may be calculated using a standard curve obtained by measuring the fluorescence polarization of a series of DON solutions of known concentration.

US Patent:

7547506, Jun 16, 2009

Filed:

Oct 31, 2002

Appl. No.:

10/492998

Inventors:

Om P. Surujballi - Nepean, CA
Anna Romanowska - Ottawa, CA
Michael E. Jolley - Round Lake IL, US
Mohammad Sarwar Nasir - Grayslake IL, US

Assignee:

Diachemix LLC - Milwaukee WI
Her Majesty the Queen in Right of Canada, as represented by the Canadian Food Inspection Agency - Ottawa (Nepean), Ontario

International Classification:

C12Q 1/00
A61K 39/04
G01N 33/566
A61K 38/00

US Classification:

435 4, 435 71, 4352531, 436501, 436543, 436546, 530300, 530350, 424 91, 424 92, 4241301, 4241501, 4241641, 4241841, 4242481

Abstract:

The present invention provides an assay for detection of -infected animals. A tracer, comprising a peptide of protein MPB70 conjugated to a fluorophore, is added to a serum sample from an animal to form a mixture. The fluorescence polarization of the mixture in then measured and compared to the fluorescence polarization of a control. The present invention further provides a tracer for use in fluorescence polarization assay to detect antibodies specific for The tracer comprises a peptide of protein MPB70 conjugated to a fluorophore, such that the tracer is able to bind to antibodies specific for to produce a detectable change in fluorescence polarization.

US Patent:

7811775, Oct 12, 2010

Filed:

May 27, 2008

Appl. No.:

12/127350

Inventors:

Mohammad Sarwar Nasir - Grayslake IL, US
Michael E. Jolley - Round Lake IL, US

Assignee:

Diachemix LLC - Milwaukee WI

International Classification:

G01N 33/53

US Classification:

435 71, 435 792, 435 793, 435 794, 436164, 436172, 436501

Abstract:

A homogeneous assay for determining the aflatoxin content in agricultural products uses the technique of fluorescence polarization. A solvent is used to extract aflatoxins from a sample of the agricultural product. A mixture is prepared by combining the extract with a tracer and with a monoclonal antibody specific for aflatoxin. The tracer is able to bind to the monoclonal antibody to produce a detectable change in fluorescence polarization. The tracer is prepared by conjugating an aflatoxin oxime to a suitable fluorophore. The fluorescence polarization of the mixture is measured. The aflatoxin concentration of the mixture may be calculated using a standard curve obtained by measuring the fluorescence polarization of a series of aflatoxin solutions of known concentration.

US Patent:

20020025512, Feb 28, 2002

Filed:

Sep 21, 1999

Appl. No.:

09/400564

Inventors:

RONALD C. MONTELARO - WEXFORD PA, US
SARA B. TENCZA - PITTSBURGH PA, US
MICHAEL E. JOLLEY - ROUND LAKE IL, US
MOHAMMAD S. NASIR - GRAYSLAKE IL, US

International Classification:

C12Q001/70
C12P013/14

US Classification:

435/005000

Abstract:

A fluorescence polarization assay for Equine Infectious Anemia Virus utilizes a short peptide reagent probe derived from a conserved immunodominant region of gp45. The probe is N-terminally labeled, preferably with 6-carboxyfluorescein, and purified by HPLC, which reacts in a homogenous assay with anti-EIAV antibodies contained in the serum of field infected horses and ponies. The assay has a sensitivity of about 90 percent with a specificity approaching 100 percent.

US Patent:

20040137525, Jul 15, 2004

Filed:

Oct 14, 2003

Appl. No.:

10/686053

Inventors:

Michael Jolley - Round Lake IL, US
Mohammad Nasir - Grayslake IL, US

International Classification:

G01N033/53

US Classification:

435/007100

Abstract:

A homogeneous fluorescence polarization inhibition assay is used to test for Salmonella contamination, e.g., Salmonella cells, in a sample. The assay makes use of a tracer comprising a fluorophore conjugated to an oligosaccharide from a Salmonella cell wall lipopolysaccharide. The sample is added to an anti-Salmonella antibody to form a mixture, and a blank fluorescence polarization measurement is taken. The tracer is then added to the mixture. After incubation, the fluorescence polarization of the mixture is measured and the blank reading is subtracted. The level of Salmonella contamination in the sample may be determined from the fluorescence polarization measured in this way.

US Patent:

20040185499, Sep 23, 2004

Filed:

Mar 20, 2003

Appl. No.:

10/393134

Inventors:

Michael Jolley - Round Lake IL, US
Mohammad Nasir - Grayslake IL, US

International Classification:

G01N033/53

US Classification:

435/007100

Abstract:

An antigenic protein includes a known amino acid sequence. To locate one or more epitopes of the antigenic protein, a plurality of distinct peptides are synthesized bound to respective solid-phase supports via selectively cleavable linkers. Each of the distinct peptides corresponds to a sub-sequence of the antigenic protein's known amino acid sequence. While the peptides are bound to their respective supports, they are conjugated to a fluorophore. The conjugated peptides are then selectively cleaved from their supports, and the fluorescence polarization of the free conjugated peptides is measured. The free conjugated peptides are each combined with an antibody that is able to bind to the antigenic protein, and the fluorescence polarization of the mixtures is measured. A substantial increase in fluorescence polarization of a mixture indicates the presence of an epitope.

Address:

1536 Chippewa Trl, Wheeling, IL 60090

Phone:

847-398-3938

VIN:

1G2ZF58B474222749

Make:

PONTIAC

Model:

G6

Year:

2007