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Michael W Hunkapiller

age ~73

from San Carlos, CA

Also known as:
  • Michael Hunkapiller
  • Mike Hunkapiller
  • Michael R
  • Mic Hunkapiller
1333 Pebble Dr, San Carlos, CA 94070

Michael Hunkapiller Phones & Addresses

  • 1333 Pebble Dr, San Carlos, CA 94070
  • San Gabriel, CA
  • Pasadena, CA
  • Yonkers, NY
  • Denver, CO
  • Redwood City, CA
  • Foster City, CA
  • Norwalk, CT

Us Patents

  • Automated Dna Sequencing Technique

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  • US Patent:
    5747249, May 5, 1998
  • Filed:
    Dec 21, 1994
  • Appl. No.:
    8/361176
  • Inventors:
    Lloyd M. Smith - Madison WI
    Leroy E. Hood - Seattle WA
    Michael W. Hunkapiller - San Carlos CA
    Tim J. Hunkapiller - Seattle WA
    Charles R. Connell - Redwood City CA
  • Assignee:
    California Institute of Technology - Pasadena CA
  • International Classification:
    C12Q 168
    C12P 1934
    C07H 2104
  • US Classification:
    435 6
  • Abstract:
    A process for the electrophoretic analysis of DNA fragments produced in DNA sequencing operations wherein chromophores or fluorophores are used to tag the DNA fragments produced by the sequencing chemistry and permit the detection and characterization of the fragments as they are resolved by electrophoresis through a gel. Preferably four different fragment sets are tagged with the fluorophores fluorescein, Texas Red, tetramethyl rhodamine, and 7-nitro-benzofurazan. A system for the electrophoretic analysis of DNA fragments produced in DNA sequencing operations comprising: a source of chromophore or fluorescent tagged DNA fragments; a zone for contacting an electrophoresis gel; means for introducing said tagged DNA fragments to said zone; and photometric means for monitoring said tagged DNA fragments as they move through said gel.
  • Apparatus For The Sequential Performance Of Chemical Processes

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  • US Patent:
    4704256, Nov 3, 1987
  • Filed:
    Nov 10, 1982
  • Appl. No.:
    6/440571
  • Inventors:
    Leroy E. Hood - Pasadena CA
    Michael W. Hunkapiller - San Gabriel CA
    William J. Dreyer - Pasadena CA
    Rodney M. Hewick - Pasadena CA
    Anton W. Stark - Pasadena CA
  • Assignee:
    California Institute of Technology - Pasadena CA
  • International Classification:
    G01N 3368
  • US Classification:
    422 68
  • Abstract:
    An improved apparatus and method for the sequential performance of chemical processes on a sample of chemical material wherein the sample is embedded in a solid matrix of fluid permeable material located within a reaction chamber and is sequentially subjected to a plurality of fluids passed through the chamber in a pressurized stream, causing chemical interaction between the sample and the fluids.
  • Ligation Assembly And Detection Of Polynucleotides On Solid-Support

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  • US Patent:
    5942609, Aug 24, 1999
  • Filed:
    Nov 12, 1998
  • Appl. No.:
    9/191390
  • Inventors:
    Michael W. Hunkapiller - San Carlos CA
    Andrew C. Hiatt - San Diego CA
  • Assignee:
    The Porkin-Elmer Corporation - Foster City CA
  • International Classification:
    C07H 2100
  • US Classification:
    536 253
  • Abstract:
    The present invention concerns methods of assembly of a polynucleotide on a solid-support by performing steps of annealing, ligation, and extension. The steps may be repeated in a cyclical manner to assemble immobilized double- or single-stranded polynucleotides with functional gene properties. The immobilized polynucleotides may be amplified by the polymerase chain reaction, and detected and quantitated by an exonuclease assay with a self-quenching, fluorescent probe. The polynucleotide may be cleaved from the solid-support by chemical or enzymatic cleavage.
  • Automated System For Providing A Sequence Of Chemicals To A Reaction Process

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  • US Patent:
    5273715, Dec 28, 1993
  • Filed:
    Nov 6, 1992
  • Appl. No.:
    7/973137
  • Inventors:
    John Bridgham - Palo Alto CA
    Timothy G. Geiser - La Honda CA
    Michael W. Hunkapiller - San Carlos CA
    Stephen B. H. Kent - Pasadena CA
    Paul O. Ramstad - Oakland CA
  • Assignee:
    Applied Biosystems, Inc. - Foster City CA
  • International Classification:
    G01N 2100
    G01N 3100
    B01L 302
    B01L 1100
  • US Classification:
    422 63
  • Abstract:
    An automated system for providing a preselected sequence of chemicals to a reaction. This apparatus includes a track on which a set of cartridges are placed in a preselected order corresponding to an order in which they are to be used in a reaction process. These cartridges are moved past a point at which these chemicals are extracted for use in the process. The chemicals are preferably in liquid form and are contained in containing having a top seal through which a needle can penetrate to extract chemicals for use in the process. These containers preferably contain the aliquot portion needed for the process, thereby providing a mechanism for providing accurate amounts of each chemical to the process.
  • Conversion Flask For Sequential Performance Apparatus

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  • US Patent:
    4610847, Sep 9, 1986
  • Filed:
    Apr 23, 1984
  • Appl. No.:
    6/602699
  • Inventors:
    Leroy E. Hood - Pasadena CA
    Michael W. Hunkapiller - San Gabriel CA
    William J. Dreyer - Pasadena CA
    Rodney M. Hewick - Pasadena CA
    Anton W. Stark - Pasadena CA
  • Assignee:
    California Institute of Technology - Pasadena CA
  • International Classification:
    B01J 1926
    G01N 3368
  • US Classification:
    422102
  • Abstract:
    Conversion flask for use is an apparatus for the sequential performance of chemical processes on a sample of chemical material. The flask includes at least three capillary tubes and a large bore tube extending into the interior thereof for the introduction and withdrawal of various fluids. The capillary tubes are constructed to introduce a plurality of fine bubbles into a liquid within the flask to agitate the liquid and accelerate drying, to impinge chemicals on the walls of the flask, and to produce a spray onto the interior walls of the flask in proximity to its upper end to wash down the interior walls of the flask.
  • Automated Dna Sequencing Technique

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  • US Patent:
    5171534, Dec 15, 1992
  • Filed:
    Oct 15, 1990
  • Appl. No.:
    7/558312
  • Inventors:
    Lloyd M. Smith - Madison WI
    Leroy E. Hood - Pasadena CA
    Michael W. Hunkapiller - San Carlos CA
    Tim J. Hunkapiller - Pasadena CA
  • Assignee:
    California Institute of Technology - Pasadena CA
  • International Classification:
    C07K 314
    G01N 33561
  • US Classification:
    422 8205
  • Abstract:
    A system for the electrophoretic analysis of DNA fragments produced in DNA sequencing operations comprising: a source of chromophore or fluorescent tagged DNA fragments; a zone for contacting an electrophoresis gel; means for introducing said tagged DNA fragments to said zone; and photometric means for monitoring said tagged DNA fragments as they move through said gel.
  • Detection Of Specific Sequences In Nucleic Acids

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  • US Patent:
    4883750, Nov 28, 1989
  • Filed:
    Dec 13, 1984
  • Appl. No.:
    6/681055
  • Inventors:
    Norman M. Whiteley - San Carlos CA
    Michael W. Hunkapiller - San Carlos CA
    Alexander N. Glazer - Orinda CA
  • Assignee:
    Applied Biosystems, Inc. - Foster City CA
  • International Classification:
    C12Q 168
  • US Classification:
    435 6
  • Abstract:
    The invention provides a method for diagnosis of genetic abnormalities or other genetic conditions which can be readily automated. The method is used to determine the presence or absence of a target sequence in a sample of denatured nucleic acid and entails hybridizing the sample with a probe complementary to a diagnostic portion of the target sequence (the diagnostic probe), and with a probe complementary to a nucleotide sequence contiguous with the diagnostic portion (the contiguous probe), under conditions wherein the diagnostic probe remains bound substantially only to the sample nucleic acid containing the target sequence. The diagnostic probe and contiguous probe are then covalently attached to yield a target probe which is complementary to the target sequence, and the probes which are not attached are removed. In the preferred mode, one of the probes is labeled so that the presence or absence of the target sequence can then be tested by melting the sample nucleic acid-target probe duplex, eluting the dissociated target probe, and testing for the label. In another embodiment, the testing is accomplished without first removing probes not covalently attached, by attaching a hook to the probe that is not labeled, so that the labeled target probe may be recovered by catching the hook.
  • Detection Of Specific Sequences In Nucleic Acids

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  • US Patent:
    5962223, Oct 5, 1999
  • Filed:
    Dec 19, 1995
  • Appl. No.:
    8/574826
  • Inventors:
    Norman M. Whiteley - San Carlos CA
    Michael W. Hunkapiller - San Carlos CA
    Alexander N. Glazer - Orinda CA
  • Assignee:
    The Perkin-Elmer Corporation - Foster City CA
  • International Classification:
    C12Q 168
    C07H 2104
    C12P 1934
  • US Classification:
    435 6
  • Abstract:
    The invention provides a method for diagnosis of genetic abnormalities or other genetic conditions which can be readily automated. The method is used to determine the presence or absence of a target sequence in a sample of denatured nucleic acid and entails hybridizing the sample with a probe complementary to a diagnostic portion of the target sequence (the diagnostic probe), and with a probe complementary to a nucleotide sequence contiguous with the diagnostic portion (the contiguous probe), under conditions wherein the diagnostic probe remains bound substantially only to the sample nucleic acid containing the target sequence. The diagnostic probe and contiguous probe are then covalently attached to yield a target probe which is complementary to the target sequence, and the probes which are not attached are removed. In the preferred mode, one of the probes is labeled so that the presence or absence of the target sequence can then be tested by melting the sample nucleic acid-target probe duplex, eluting the dissociated target probe, and testing for the label. In another embodiment, the testing is accomplished without first removing probes not covalently attached, by attaching a hook to the probe that is not labeled, so that the labeled target probe may be recovered by catching the hook.

Wikipedia

Applied Biosystems

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In 1995, upon Andre Marion retirement, Mike Hunkapiller became President of PE .... Dr. Michael W. Hunkapiller remained as a Senior Vice President of PE ...


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