John Coller - Redwood City CA, US Michael Fero - Portola Valley CA, US Ian Hsu - 61-1 East Yun, TW Ching-Lung Huang - Tainan, TW
International Classification:
H01S 3/00
US Classification:
359341100
Abstract:
The present invention provides a method and device for optically extracting discrete features of an object. With this method and device, small regions of interest or features within a relatively large object are optically extracted and collated into a single, condensed image. The condensed image contains all of the features in the original object, but not the parts of the object that are between the features. Thus, the optically extracted features can be viewed at high resolution, but in a limited field of view. Examples of objects that may be optically extracted according to the invention include spots of biomaterial on a biochip, material in wells of a microtiter plate, etc. In addition, this device and method can be used for non-planar objects. In this case, the device and method allow simultaneous visualization of spatially separated features on a surface of a 3-dimensional object.
Compositions And Methods For Identifying The Essential Genome Of An Organism
Beat Christen - San Francisco CA, US Michael Fero - San Francisco CA, US Eduardo Abeliuk - Portola Valley CA, US
Assignee:
QB3/Pharm-Chem Digital Health Garage - San Francisco CA
International Classification:
C12Q 1/68 G06F 19/22
US Classification:
506 2, 4353201, 435 912, 702 20
Abstract:
Compositions and methods are provided for the rapid and highly accurate identification of the entire essential genome of any organism under a given selection condition at a resolution of a few base pairs. An engineered transposon bearing an adapter sequence for ultra high throughput adaptor-based sequencing is employed for hyper-saturated transposon mutagenesis. Transposon junctions are subsequently isolated and collectively amplified through a shared parallel PCR strategy such that a second adaptor sequence is further incorporated into template DNA so that the first adaptor sequence and the second adaptor sequence flank the 5′ and 3′ regions of the sample DNA, respectively. Sample DNA is then sequenced in an ultra high-throughput adaptor-based DNA sequencer using adaptor primers. Transposon insertion sites are mapped onto the organism's genome, allowing for the algorithmic identification of essential genetic elements based on genomic transposition frequency.
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Company / Classification
Phones & Addresses
Michael P. Fero Managing
Sierra Surgical LLC Medical Device Sales · Medical Device Sales and Consulting
3958 Foothill Ave, Carlsbad, CA 92010
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