Mark W Renshaw

US Patent:

7396917, Jul 8, 2008

Filed:

Dec 2, 2002

Appl. No.:

10/307724

Inventors:

Katherine S. Bowdish - Del Mar CA, US
Shana Frederickson - Solana Beach CA, US
Mark Renshaw - San Diego CA, US

Assignee:

Alexion Pharmaceuticals, Inc. - Cheshire CT

International Classification:

C07K 16/00

US Classification:

5303891, 5303871, 5303873, 5303875, 53038815, 53038824, 5303892

Abstract:

Antibodies or fragments thereof having at least two CDR regions replaced or fused with biologically active peptides are described. Compositions containing such antibodies or fragments thereof are useful in therapeutic and diagnostic modalities.

US Patent:

7414111, Aug 19, 2008

Filed:

Dec 15, 2003

Appl. No.:

10/737252

Inventors:

Toshiaki Maruyama - La Jolla CA, US
Katherine S. Bowdish - Del Mar CA, US
Shana Frederickson - Solana Beach CA, US
Mark Renshaw - San Diego CA, US

Assignee:

Alexion Pharmaceuticals, Inc. - Cheshire CT

International Classification:

C07K 16/00

US Classification:

5303871, 5303873, 5303881, 530861

Abstract:

Methods of amplifying nucleic acid have now been discovered which include the steps of: a) annealing a primer to a template nucleic acid sequence, the primer having a first portion which anneals to the template and a second portion of predetermined sequence; b) synthesizing a polynucleotide that anneals to and is complementary to the portion of the template between the location at which the first portion of the primer anneals to the template and the end of the template, the polynucleotide having a first end and a second end, wherein the first end incorporates the primer; c) separating the polynucleotide synthesized in step (b) from the template; d) annealing a nested oligonucleotide to the second end of the polynucleotide synthesized in step (b), the nested oligonucleotide having a first portion that anneals to the second end of the polynucleotide and a second portion having the same predetermined sequence as the second portion of the primer; e) extending the polynucleotide synthesized in step (b) to provide a terminal portion thereof that is complementary to the predetermined sequence; and f) amplifying the extended polynucleotide using a single primer having the predetermined sequence. In particularly useful embodiments, the methods are used to amplify a repertoire of IgA antibodies.

US Patent:

7482435, Jan 27, 2009

Filed:

Dec 5, 2001

Appl. No.:

10/006593

Inventors:

Katherine S. Bowdish - Del Mar CA, US
Shana Frederickson - Solana Beach CA, US
Mark Renshaw - San Diego CA, US

Assignee:

Alexion Pharmaceuticals, Inc. - Cheshire CT

International Classification:

C07K 16/00

US Classification:

5303881, 53038873

Abstract:

Antibodies or fragments thereof having CDR regions replaced or fused with biologically active peptides are described. Flanking sequences may optionally be attached at one or both the carboxy-terminal and amino-terminal ends of the peptide in covalent association with adjacent framework regions. Compositions containing such antibodies or fragments thereof are useful in therapeutic and diagnostic modalities.

US Patent:

20030049731, Mar 13, 2003

Filed:

Dec 5, 2001

Appl. No.:

10/006591

Inventors:

Katherine Bowdish - Del Mar CA, US
Shana Frederickson - Solana Beach CA, US
Ying-Chi Lin - San Diego CA, US
Mark Renshaw - San Diego CA, US
Martha Wild - San Diego CA, US
John McWhirter - San Diego CA, US

International Classification:

C12P021/02
C12P019/34
C12N005/06

US Classification:

435/069100, 435/091200, 435/320100, 435/326000

Abstract:

Nucleic acid sequences encoding at least a portion of a polypeptide are directly incorporated into a plasmid by DNA polymerization or reverse transcription of a nucleic acid template. In particularly preferred embodiments, nucleic acid sequences encoding at least a portion of an antibody are directly incorporated into a plasmid by reverse transcription of messenger RNA (mRNA).

US Patent:

20040072164, Apr 15, 2004

Filed:

Sep 19, 2002

Appl. No.:

10/251085

Inventors:

Toshiaki Maruyama - La Jolla CA, US
Shana Frederickson - Solana Beach CA, US
Katherine Bowdish - Del Mark CA, US
Mark Renshaw - San Diego CA, US
Ying-Chi Lin - San Diego CA, US

International Classification:

C12Q001/68
C12P019/34

US Classification:

435/006000, 435/091200

Abstract:

Methods of amplifying nucleic acid have now been discovered which include the steps of: a) annealing a primer to a template nucleic acid sequence, the primer having a first portion which anneals to the template and a second portion of predetermined sequence; b) synthesizing a polynucleotide that anneals to and is complementary to the portion of the template between the location at which the first portion of the primer anneals to the template and the end of the template, the polynucleotide having a first end and a second end, wherein the first end incorporates the primer; c) separating the polynucleotide synthesized in step (b) from the template; d) annealing a nested oligonucleotide to the second end of the polynucleotide synthesized in step (b), the nested oligonucleotide having a first portion that anneals to the second end of the polynucleotide and a second portion having the same predetermined sequence as the second portion of the primer; e) extending the polynucleotide synthesized in step (b) to provide a terminal portion thereof that is complementary to the predetermined sequence; and f) amplifying the extended polynucleotide using a single primer having the predetermined sequence.

US Patent:

20040253242, Dec 16, 2004

Filed:

Dec 15, 2003

Appl. No.:

10/737290

Inventors:

Katherine Bowdish - Del Mar CA, US
Shana Frederickson - Solana Beach CA, US
Mark Renshaw - Solana Beach CA, US
Cecelia Orencia - Del Mar CA, US

International Classification:

A61K039/395
C07K016/26

US Classification:

424/145100, 530/388240

Abstract:

Antibodies or fragments thereof having CDR regions replaced or fused with biologically active peptides are described. Flanking sequences may optionally be attached at one or both the carboxy-terminal and amino-terminal ends of the peptide in covalent association with adjacent framework regions. Compositions containing such antibodies or fragments thereof are useful in therapeutic and diagnostic modalities.

US Patent:

20090011471, Jan 8, 2009

Filed:

Jun 12, 2007

Appl. No.:

11/811835

Inventors:

Katherine S. Bowdish - Del Mar CA, US
Shana Frederickson - Solana Beach CA, US
Ying-Chi Lin - San Diego CA, US
Mark Renshaw - San Diego CA, US
Martha Wild - San Diego CA, US
John McWhirter - San Diego CA, US

International Classification:

C12P 19/34
C12N 5/10

US Classification:

435 9152, 435 9153, 435348

Abstract:

Nucleic acid sequences encoding at least a portion of a polypeptide are directly incorporated into a plasmid by DNA polymerization or reverse transcription of a nucleic acid template. In particularly preferred embodiments, nucleic acid sequences encoding at least a portion of an antibody are directly incorporated into a plasmid by reverse transcription of messenger RNA (mRNA).

US Patent:

20090130716, May 21, 2009

Filed:

Jul 1, 2008

Appl. No.:

12/217216

Inventors:

Katherine S. Bowdish - Del Mar CA, US
Shana Frederickson - Solana Beach CA, US
Mark Renshaw - San Diego CA, US

Assignee:

Alexion Pharmaceuticals, Inc. - Cheshire CT

International Classification:

C12P 21/04
C12N 15/11
C12N 15/00
C12N 5/06

US Classification:

435 696, 536 2353, 4353201, 435325

Abstract:

Antibodies or fragments thereof having at least two CDR regions replaced or fused with biologically active peptides are described. Compositions containing such antibodies or fragments thereof are useful in therapeutic and diagnostic modalities.