Krista L Witte

US Patent:

7394547, Jul 1, 2008

Filed:

Nov 4, 2004

Appl. No.:

10/981901

Inventors:

Hong Tan - San Jose CA, US
Duan Jun Chen - East Brunswick NJ, US
Yushan Tan - Shanghai, CN
Krista Leah Witte - Hayward CA, US

Assignee:

ForteBio, Inc. - Menlo Park CA

International Classification:

G01B 9/02

US Classification:

356480

Abstract:

Apparatus and method for detecting the presence or amount or rate of binding of an analyte in a sample solution is disclosed. The apparatus includes an optical assembly having first and second reflecting surfaces separated by a distance “d” greater than 50 nm, where the first surface is formed by a layer of analyte-binding molecules, and a light source for directing a beam of light onto said first and second reflecting surface. A detector in the apparatus operates to detect a change in the thickness of the first reflecting layer resulting from binding of analyte to the analyte-binding molecules, when the assembly is placed in the solution of analyte, by detecting a shift in phase of light waves reflected from the first and second surfaces.

US Patent:

7445887, Nov 4, 2008

Filed:

Jan 6, 2006

Appl. No.:

11/326689

Inventors:

Robert Zuk - Atherton CA, US
Sae Choo - San Jose CA, US
Weilei Ma - Palo Alto CA, US
Krista Witte - Hayward CA, US

Assignee:

ForteBio, Inc. - Menlo Park CA

International Classification:

C12Q 1/00

US Classification:

435 4, 356901

Abstract:

Disclosed are enzyme assays using biolayer interferometry. Assays may be carried out using immobilized substrate or with a substrate capture format. In certain embodiments, the assays are carried out using unlabeled substrates. The methods are broadly applicable to enzyme assay measurements, can be carried out in vivo or in vitro, and are easily multiplexed.

US Patent:

7656536, Feb 2, 2010

Filed:

Dec 14, 2007

Appl. No.:

11/957340

Inventors:

Hong Tan - San Jose CA, US
Yushan Tan - Shanghai, CN
Krista Leah Witte - Hayward CA, US
Greg L. Carricato - San Jose CA, US
Scott Lockard - Los Gatos CA, US

Assignee:

Fortebio, Inc. - Menlo Park CA

International Classification:

G01B 9/02

US Classification:

356478, 356480

Abstract:

Apparatus and method for detecting an analyte in a sample based on optical interference. The apparatus includes a light source, detector unit and one or more disposable detector tips. The apparatus also includes an optical coupling assembly that couples light from the source to the detector tips, and from the detector tips to the detector unit.

US Patent:

7728982, Jun 1, 2010

Filed:

Apr 8, 2008

Appl. No.:

12/099751

Inventors:

Hong Tan - San Jose CA, US
Yushan Tan - Shanghai, CN
Duan Jun Chen - East Brunswick NJ, US
Krista Leah Witte - Hayward CA, US

Assignee:

ForteBio, Inc. - Menlo Park CA

International Classification:

G01B 9/02

US Classification:

356478, 356480

Abstract:

Apparatus and method for detecting the presence or amount or rate of binding of an analyte in a sample solution is disclosed. The apparatus includes an optical assembly having first and second reflecting surfaces separated by a distance “d” greater than 50 nm, where the first surface is formed by a layer of analyte-binding molecules, and a light source for directing a beam of light onto said first and second reflecting surface. A detector in the apparatus operates to detect a change in the thickness of the first reflecting layer resulting from binding of analyte to the analyte-binding molecules, when the assembly is placed in the solution of analyte, by detecting a shift in phase of light waves reflected from the first and second surfaces.

US Patent:

8305585, Nov 6, 2012

Filed:

May 28, 2010

Appl. No.:

12/790736

Inventors:

Hong Tan - San Jose CA, US
Yushan Tan - Shanghai, CN
Duan Jun Chen - East Brunswick NJ, US
Krista Leah Witte - Hayward CA, US

Assignee:

Pall Corporation - Port Washington NY

International Classification:

G01B 9/02

US Classification:

356478, 356480

Abstract:

Apparatus and method for detecting the presence or amount or rate of binding of an analyte in a sample solution is disclosed. The apparatus includes an optical assembly having first and second reflecting surfaces separated by a distance “d” greater than 50 nm, where the first surface is formed by a layer of analyte-binding molecules, and a light source for directing a beam of light onto said first and second reflecting surface. A detector in the apparatus operates to detect a change in the thickness of the first reflecting layer resulting from binding of analyte to the analyte-binding molecules, when the assembly is placed in the solution of analyte, by detecting a shift in phase of light waves reflected from the first and second surfaces.

US Patent:

8647588, Feb 11, 2014

Filed:

May 4, 2011

Appl. No.:

13/101112

Inventors:

Michael W. Recknor - Oakland CA, US
Hong Tan - San Jose CA, US
Robert Zuk - Atherton CA, US
Krista Leah Witte - Hayward CA, US
Sae Choo - Sunnyvale CA, US
Scott Lockard - Los Gatos CA, US

Assignee:

Pall Corporation - Port Washington NY

International Classification:

G01N 21/75
B01L 3/00
C12Q 1/68

US Classification:

422407, 422 50, 422400, 422401, 422408, 422417, 422503, 422504, 422511, 422521

Abstract:

An apparatus and method for packaging of an optical sensing fiber is disclosed. The apparatus includes a substrate with a plurality of openings, and each opening is configured for holding an optical sensing assembly. The assembly is positioned in the opening with a tip of the assembly extending through the opening to be suspended from the substrate. In addition, openings are arranged so the assembly positioned therein avoids contacting another assembly positioned therein. The apparatus can include a support member for supporting the substrate and positioning the substrate so the tip of the assembly suspended from the opening in the substrate contacts solution in one of a plurality of wells in a container adjacent to the substrate. The assembly can be configured for preparing of the optical assembly for assay. An agitation assembly for agitating the container to create flow of the solution in the container wells over an optical sensing assembly is also disclosed.

US Patent:

20070070356, Mar 29, 2007

Filed:

Jun 12, 2006

Appl. No.:

11/423671

Inventors:

Hong Tan - San Jose CA, US
Yushan Tan - Shanghai, CN
Krista Witte - Hayward CA, US
Robert Zuk - Atherton CA, US
Greg Carricato - San Jose CA, US
Scott Lockard - Los Gatos CA, US

International Classification:

G01B 9/02
G01N 21/00
G01N 21/03
B32B 27/04

US Classification:

356477000, 422082050, 436165000

Abstract:

Apparatus and method for detecting an analyte in a sample based on optical interference. The apparatus includes a light source, detector unit and one or more disposable detector tips. The apparatus also includes an optical coupling assembly that couples light from the source to the detector tips, and from the detector tips to the detector unit.

US Patent:

20070161042, Jul 12, 2007

Filed:

Jan 11, 2006

Appl. No.:

11/330925

Inventors:

Robert Zuk - Atherton CA, US
Krista Witte - Hayward CA, US
Bettina Heidecker - Mountain View CA, US

Assignee:

ForteBio, Inc. - Menlo Park CA

International Classification:

G01N 33/53

US Classification:

435007100

Abstract:

Methods are provided for measuring rate constants for high affinity molecular interactions using an assay format for determining dissociation rates in liquid phase. The invention uses a biosensor that at selected time intervals is contacted with a sample solution to estimate the ratio of bound vs. free ligand. Dissociation rate constants determined according to the methods of the invention more closely mimic in vivo binding constants and avoid diffusional barrier artifacts that accompany measurements performed using solid phase devices. The methods of the invention provide further advantage by not requiring continuous measurements be made on a biosensor instrument thus leaving it available to process other samples. The methods permit accurate determination of dissociation rates of reactions for which dissociation slowly occurs over intervals of hours to days or more.