Jon A Kornacki

US Patent:

52273008, Jul 13, 1993

Filed:

Oct 23, 1992

Appl. No.:

7/966652

Inventors:

Paul E. Holmes - Hamden CT
Jon A. Kornacki - Wallingford CT

Assignee:

Olin Corporation - Cheshire CT

International Classification:

C12N 920

US Classification:

435198

Abstract:

A novel lipase from a newly-discovered strain of Pseudomonas alcaligenes microorganism having (i) an optimum pH for activity of about 10. +-. 5; (ii) an optimum temperature for activity of about 45. degree. to 55. degree. C. ; (iii) an optimum pH for stability of about 7. +-. 5; (iv) a molecular weight as measured by SDS-PAGE of about 3. times. 10. sup. 4 ; and (v) chemical stability for at least a 60 day mean half-life in the presence of a 10 percent solution of polyoxyethylene (23) lauryl ether in 25 millimolar aqueous calcium chloride. Also claimed is a biologically pure culture of the microorganism, and a method for the production of the lipase. Also claimed is a lipase characterized by containing an N-terminal amino acid sequence which is Gly-Leu-Phe-Gly-Pro-Ser-Gly-Tyr-Thr-Lys-Thr-Lys-Tyr-Pro-Ile.