Jeffrey M Staub

US Patent:

6388168, May 14, 2002

Filed:

Nov 18, 1998

Appl. No.:

09/193853

Inventors:

Pal Maliga - East Brunswick NJ
Zora Svab Maliga - East Brunswick NJ
Jeffrey M. Staub - Wildwood MO

Assignee:

Rutgers, The State University of New Jersey - New Brunswick NJ

International Classification:

C12N 1529

US Classification:

800278, 800287, 800288, 536 236, 536 237, 536 2372, 536 241, 435 691, 4353201, 435468

Abstract:

DNA constructs are provided for stable transformation of plastids of multicellular plants and expression of foreign proteins in plastids. The DNA constructs comprise a transforming DNA which is targeted to a pre-determined location on the plastid genome and inserted into the plastid genome by homologous recombination with targeting segments comprising DNA sequences homologous to the pre-determined region of the plastid genome. The transforming DNA contains a non-lethal selectable marker gene which confers a selectable phenotype on cells having plastids in which substantially all of the genomes therein contain the transforming DNA (i. e. , homoplasmic cells or tissues). The transforming DNA further comprises at least one insertion site 4 for an additional DNA segment, such as a gene encoding a protein for improving a characteristic of the transformed plant. The non-lethal selectable marker gene is preferably provided as a chimeric gene by assembly from an expression cassette comprising 5â and 3â regulatory segments, preferably derived from plastid genes. A coding segment encoding the non-lethal selectable marker is inserted between the 5â and 3â regulatory segments to form the chimeric gene.

US Patent:

6492578, Dec 10, 2002

Filed:

Jul 10, 1999

Appl. No.:

09/351123

Inventors:

Jeffrey M. Staub - Chesterfield MO
Peter Hajdukiewicz - Chesterfield MO
Kevin E. McBride - Davis CA
Narender Nehra - Chesterfield MO
David J. Schaaf - Davis CA
David M. Stalker - Woodland CA
Guangning Ye - Ellisville MO

Assignee:

Calgene LLC - Davis CA

International Classification:

C12N 1582

US Classification:

800300, 800278, 800287, 800288, 435 691, 435418, 435419, 435468, 536 232, 536 236, 536 237, 536 241, 536 2372

Abstract:

Provided are constructs and methods for expressing herbicide tolerance genes in plastids of plant cells. Constructs include the components of a promoter functional in a plant plastid, a DNA sequence which is capable of conferring tolerance in a plant cell to at least one herbicide compound when said DNA sequence is transcribed in plastids of said plant cell and a transcription termination region. Herbicide tolerance is produced by transforming plastids with the constructs of the invention and growing plant cells comprising the transformed plastids under conditions wherein the DNA sequence is transcribed and plant plastids and cells containing the plastids are rendered tolerant to applications of at least one herbicide compound.

US Patent:

6512162, Jan 28, 2003

Filed:

Jul 10, 1998

Appl. No.:

09/113244

Inventors:

Kevin E. McBride - Davis CA
Narender Nehra - Chesterfield MO
Douglas A. Russell - Madison WI
David M. Stalker - Woodland CA
Jeffrey M. Staub - Chesterfield MO

Assignee:

Calgene LLC - Davis CA

International Classification:

C12N 1582

US Classification:

800278, 800287, 800288, 435419, 435468, 536 236, 536 237, 536 2372, 536 241, 536 235

Abstract:

Constructs and methods are provided for expressing peptides derived from eukaryotic organisms in plant plastids. Constructs have a promoter functional in a plant plastid, a DNA sequence encoding a peptide derived from an eukaryotic organism and a transcription termination region. Other elements include a selectable marker for selection of plant cells comprising a plastid expressing the marker and DNA regions of homology to the genome of the plastid and optionally a ribosome binding site joined to the promoter. By methods using such constructs high levels of eukaryotic peptides, such as mammalian proteins, are produced in a plant cell by growing plant cells under conditions whereby the DNA encoding sequences re expressed to produce eukaryotic peptide in said plastid.

US Patent:

6781033, Aug 24, 2004

Filed:

Apr 25, 2001

Appl. No.:

09/843324

Inventors:

Jeffrey M Staub - Wildwood MO
Guangning Ye - Ellisville MO
Debra L. Broyles - Wright City MO

Assignee:

Monsanto Technology LLC - St. Louis MO

International Classification:

A01H 100

US Classification:

800278, 800288

Abstract:

Novel compositions and methods useful for genetic engineering of plant cells to provide a method of producing plastid transformed plants are provided in the instant invention. In particular, the present invention provides methods for obtaining plastid transformed plants on medium containing plastid lethal compounds.

US Patent:

6812379, Nov 2, 2004

Filed:

Mar 20, 2002

Appl. No.:

10/103516

Inventors:

Jeffrey M. Staub - Chesterfield MO

Assignee:

Calgene LLC - Davis CA

International Classification:

C12N 1582

US Classification:

800278, 800288, 435 691, 435 694, 435 697, 536 2351, 536 236, 536 2372

Abstract:

Constructs and methods are provided for expressing peptides derived from eukaryotic organisms in plant plastids. Constructs have a promoter functional in a plant plastid, a DNA sequence encoding a peptide derived from an eukaryotic organism and a transcription termination region. Other elements include a selectable marker for selection of plant cells comprising a plastid expressing the marker and DNA regions of homology to the genome of the plastid and optionally a ribosome binding site joined to the promoter. By methods using such constructs high levels of eukaryotic peptides, such as mammalian proteins, are produced in a plant cell by growing plant cells under conditions whereby the DNA encoding sequences are expressed to produce eukaryotic peptide in said plastid.

US Patent:

6849778, Feb 1, 2005

Filed:

Oct 16, 2000

Appl. No.:

09/688851

Inventors:

Jeffrey M. Staub - Chesterfield MO, US
Peter H. J. Hajdukiewicz - Chesterfield MO, US
Larry Gilbertson - Chesterfield MO, US

Assignee:

Calgene LLC - Davis CA

International Classification:

A01H 100
C12N 1582
C12N 1574
C12N 1587
C07H 2104

US Classification:

800278, 800298, 800300, 8003173, 4353201, 435419, 536 231

Abstract:

Novel compositions and methods useful for genetic engineering of plant cells are provided. In particular, plastid constructs comprising recombining sites are provided. Such constructs find use in methods for site specific recombination in plant cell plastids. Plant cells and plants comprising the constructs described herein, as well as plant cells and plants produced by the methods of the present invention are of interest.

US Patent:

7259293, Aug 21, 2007

Filed:

Aug 13, 2004

Appl. No.:

10/917774

Inventors:

Jeffrey M. Staub - Chesterfield MO, US

Assignee:

Calgene LLC - St. Louis MO

International Classification:

C12N 15/82
C12N 15/62
C12N 15/18
C12N 5/04
C12N 5/10
A01H 5/00
A01H 5/10

US Classification:

800278, 800288, 435 691, 435 694, 435 697, 435419, 536 2351, 536 236, 536 2372, 536 241

Abstract:

Constructs and methods are provided for expressing peptides derived from eukaryotic organisms in plant plastids. Constructs have a promoter functional in a plant plastid, a DNA sequence encoding a peptide derived from an eukaryotic organism and a transcription termination region. Other elements include a selectable marker for selection of plant cells comprising a plastid expressing the marker and DNA regions of homology to the genome of the plastid and optionally a ribosome binding site joined to the promoter. By methods using such constructs high levels of eukaryotic peptides, such as mammalian proteins, are produced in a plant cell by growing plant cells under conditions whereby the DNA encoding sequences are expressed to produce eukaryotic peptide in said plastid.

US Patent:

7732662, Jun 8, 2010

Filed:

Aug 6, 2007

Appl. No.:

11/834512

Inventors:

Jeffrey M Staub - Wildwood MO, US
Guangning Ye - Ellisville MO, US
Debra L. Broyles - Wright City MO, US

Assignee:

Monsanto Technology LLC - St. Louis MO

International Classification:

C12N 15/82

US Classification:

800278, 435468

Abstract:

Novel compositions and methods useful for genetic engineering of plant cells to provide a method of producing plastid transformed plants are provided in the instant invention. In particular, the present invention provides methods for obtaining plastid transformed plants on medium containing plastid lethal compounds.