DNA constructs are provided for stable transformation of plastids of multicellular plants and expression of foreign proteins in plastids. The DNA constructs comprise a transforming DNA which is targeted to a pre-determined location on the plastid genome and inserted into the plastid genome by homologous recombination with targeting segments comprising DNA sequences homologous to the pre-determined region of the plastid genome. The transforming DNA contains a non-lethal selectable marker gene which confers a selectable phenotype on cells having plastids in which substantially all of the genomes therein contain the transforming DNA (i. e. , homoplasmic cells or tissues). The transforming DNA further comprises at least one insertion site 4 for an additional DNA segment, such as a gene encoding a protein for improving a characteristic of the transformed plant. The non-lethal selectable marker gene is preferably provided as a chimeric gene by assembly from an expression cassette comprising 5â and 3â regulatory segments, preferably derived from plastid genes. A coding segment encoding the non-lethal selectable marker is inserted between the 5â and 3â regulatory segments to form the chimeric gene.
Expression Of Herbicide Tolerance Genes In Plant Plastids
Jeffrey M. Staub - Chesterfield MO Peter Hajdukiewicz - Chesterfield MO Kevin E. McBride - Davis CA Narender Nehra - Chesterfield MO David J. Schaaf - Davis CA David M. Stalker - Woodland CA Guangning Ye - Ellisville MO
Provided are constructs and methods for expressing herbicide tolerance genes in plastids of plant cells. Constructs include the components of a promoter functional in a plant plastid, a DNA sequence which is capable of conferring tolerance in a plant cell to at least one herbicide compound when said DNA sequence is transcribed in plastids of said plant cell and a transcription termination region. Herbicide tolerance is produced by transforming plastids with the constructs of the invention and growing plant cells comprising the transformed plastids under conditions wherein the DNA sequence is transcribed and plant plastids and cells containing the plastids are rendered tolerant to applications of at least one herbicide compound.
Expression Of Eukaryotic Peptides In Plant Plastids
Kevin E. McBride - Davis CA Narender Nehra - Chesterfield MO Douglas A. Russell - Madison WI David M. Stalker - Woodland CA Jeffrey M. Staub - Chesterfield MO
Constructs and methods are provided for expressing peptides derived from eukaryotic organisms in plant plastids. Constructs have a promoter functional in a plant plastid, a DNA sequence encoding a peptide derived from an eukaryotic organism and a transcription termination region. Other elements include a selectable marker for selection of plant cells comprising a plastid expressing the marker and DNA regions of homology to the genome of the plastid and optionally a ribosome binding site joined to the promoter. By methods using such constructs high levels of eukaryotic peptides, such as mammalian proteins, are produced in a plant cell by growing plant cells under conditions whereby the DNA encoding sequences re expressed to produce eukaryotic peptide in said plastid.
Method For The Transformation Of Plant Cell Plastids
Jeffrey M Staub - Wildwood MO Guangning Ye - Ellisville MO Debra L. Broyles - Wright City MO
Assignee:
Monsanto Technology LLC - St. Louis MO
International Classification:
A01H 100
US Classification:
800278, 800288
Abstract:
Novel compositions and methods useful for genetic engineering of plant cells to provide a method of producing plastid transformed plants are provided in the instant invention. In particular, the present invention provides methods for obtaining plastid transformed plants on medium containing plastid lethal compounds.
Expression Of Eukaryotic Peptides In Plant Plastids
Constructs and methods are provided for expressing peptides derived from eukaryotic organisms in plant plastids. Constructs have a promoter functional in a plant plastid, a DNA sequence encoding a peptide derived from an eukaryotic organism and a transcription termination region. Other elements include a selectable marker for selection of plant cells comprising a plastid expressing the marker and DNA regions of homology to the genome of the plastid and optionally a ribosome binding site joined to the promoter. By methods using such constructs high levels of eukaryotic peptides, such as mammalian proteins, are produced in a plant cell by growing plant cells under conditions whereby the DNA encoding sequences are expressed to produce eukaryotic peptide in said plastid.
Methods And Vectors For Site-Specific Recombination In Plant Cell Plastids
Novel compositions and methods useful for genetic engineering of plant cells are provided. In particular, plastid constructs comprising recombining sites are provided. Such constructs find use in methods for site specific recombination in plant cell plastids. Plant cells and plants comprising the constructs described herein, as well as plant cells and plants produced by the methods of the present invention are of interest.
Expression Of Eukaryotic Peptides In Plant Plastids
Constructs and methods are provided for expressing peptides derived from eukaryotic organisms in plant plastids. Constructs have a promoter functional in a plant plastid, a DNA sequence encoding a peptide derived from an eukaryotic organism and a transcription termination region. Other elements include a selectable marker for selection of plant cells comprising a plastid expressing the marker and DNA regions of homology to the genome of the plastid and optionally a ribosome binding site joined to the promoter. By methods using such constructs high levels of eukaryotic peptides, such as mammalian proteins, are produced in a plant cell by growing plant cells under conditions whereby the DNA encoding sequences are expressed to produce eukaryotic peptide in said plastid.
Method For The Transformation Of Plant Cell Plastids
Jeffrey M Staub - Wildwood MO, US Guangning Ye - Ellisville MO, US Debra L. Broyles - Wright City MO, US
Assignee:
Monsanto Technology LLC - St. Louis MO
International Classification:
C12N 15/82
US Classification:
800278, 435468
Abstract:
Novel compositions and methods useful for genetic engineering of plant cells to provide a method of producing plastid transformed plants are provided in the instant invention. In particular, the present invention provides methods for obtaining plastid transformed plants on medium containing plastid lethal compounds.
Jeffrey Staub 1974 graduate of Neshaminy High School in Langhorne, PA is on Classmates.com. See pictures, plan your class reunion and get caught up with Jeffrey and other high ...
Jeffrey Staub Staub 1992 graduate of Poquessing Elementry School in Feasterville, PA is on Classmates.com. See pictures, plan your class reunion and get caught up with Jeffrey ...