Inn H Yuk from Berkeley, CA

Serum-Free Virus Propagation Platform For A Virus Vaccine Candidate
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US Patent:

20110053248, Mar 3, 2011
Filed:

Oct 5, 2007
Appl. No.:

12/445950
Inventors:

Inn Yuk - Berkeley CA, US
Wenlin Zeng - Union City CA, US
Misa Sugui - Santa Clara CA, US
Assignee:

MEDIMMUNE, LLC - Gaithersburg MD
International Classification:

C12N 7/00
C12N 5/071
US Classification:

4352351, 435364
Abstract:

The invention relates to methods for propagating viruses. In particular, the invention provides optimized conditions for propagating viruses. Optimization of the following parameters are provided: lipid concentrates as supplements to the medium, temperature shift from pre-infection to post-infection, multiplicity of infection, direct bead-to-bead transfer and serum supplementation of pre-infection medium. In particular, the invention provides for the first time a method for propagating a virus by culturing cells that are infected with the virus in a medium comprising chemically defined lipid concentrate (CDLC). In another claim, the CDLC is added to medium that is substantially free of serum for culture of virus-infected cells.

Compositions And Methods For Regulating Cell Osmolarity
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US Patent:

20110269233, Nov 3, 2011
Filed:

Sep 15, 2009
Appl. No.:

13/063912
Inventors:

Laetitia Malphettes - San Francisco CA, US
Andrew Snowden - Somerville MA, US
Inn H. Yuk - Burlingame CA, US
International Classification:

C12N 15/63
C12N 5/10
C12N 1/19
C12N 1/21
C12N 1/15
US Classification:

435440, 4353201, 43525231, 43525233, 43525235, 43525234, 4352523, 4352543, 43525421, 43525423, 4352542, 435348, 435358, 435365, 435366, 435354
Abstract:

The invention provides compositions and methods for regulating intracellular osmolarity in cells, e.g., in cultured cells, including in cultured cells in bioreactors. The invention provides nucleic acids comprising at least one osmo-responsive transcriptional regulatory element (OR-TRE), and cells, vectors, products of manufacture, artificial organs or implants and the like containing an osmo-responsive transcriptional regulatory element (OR-TRE).

Method Of Culturing Eukaryotic Cells
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US Patent:

20120329151, Dec 27, 2012
Filed:

Jul 6, 2010
Appl. No.:

13/383829
Inventors:

Dinesh Baskar - San Mateo CA, US
Jenny Hsiung - Mountain View CA, US
Inn H. Yuk - Berkeley CA, US
Assignee:

F. HOFFMANN-LA ROCHE AG - Basel
International Classification:

C12N 5/071
C12M 3/00
US Classification:

435350, 435325, 435363, 435366, 435351, 435352, 4352891
Abstract:

An apparatus and method to maintain pH within a range conducive for cell growth in a bicarbonate-containing cell culture system without the addition of base. The method relies on the gas transfer characteristics of the bioreactor system to modulate the CO2 transfer to and from the cell culture such that the pH of the cell culture can be maintained within a desired range.

Methods Of Protein Purification
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US Patent:

20100256336, Oct 7, 2010
Filed:

Sep 17, 2007
Appl. No.:

12/441775
Inventors:

Inn Huam Yuk - Berkeley CA, US
Bradley Richard Snedecor - Portola Valley CA, US
Dana Christian Andersen - Menlo Park CA, US
International Classification:

C07K 16/28
C12P 21/02
C12P 21/08
US Classification:

5303873, 435 711, 530350, 5303871
Abstract:

A method of reducing glycation of an amino acid in a protein produced by a host cell in cell culture medium is disclosed.

Modified Mammalian Cells
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US Patent:

20210309989, Oct 7, 2021
Filed:

Mar 26, 2021
Appl. No.:

17/213611
Inventors:

- South San Francisco CA, US
Shahram MISAGHI - South San Francisco CA, US
Amy SHEN - South San Francisco CA, US
Anthony TOMLINSON - South San Francisco CA, US
Inn Huam YUK - South San Francisco CA, US
Assignee:

GENENTECH, INC. - South San Francisco CA
International Classification:

C12N 15/10
C12N 5/071
Abstract:

The present disclosure relates to methods, cells, and compositions for producing a product of interest, e.g., a recombinant protein. In particular, the present disclosure provides improved mammalian cells expressing the product of interest, where the cells (e.g., Chinese Hamster Ovary (CHO) cells) have reduced or eliminated activity, e.g., expression, of certain host cell proteins, e.g., enzymes including, but not limited to, certain lipases, esterases, and/or hydrolases.

Method Of Culturing Eukaryotic Cells
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US Patent:

20210130759, May 6, 2021
Filed:

Jan 13, 2021
Appl. No.:

17/148190
Inventors:

- South San Francisco CA, US
Jenny HSIUNG - Mountain View CA, US
Inn H. YUK - Berkeley CA, US
Assignee:

Genentech, Inc. - South San Francisco CA
International Classification:

C12M 1/00
C12M 3/06
C12M 1/34
C12M 1/36
C12N 5/00
Abstract:

An apparatus and method to maintain pH within a range conducive for cell growth in a bicarbonate-containing cell culture system without the addition of base. The method relies on the gas transfer characteristics of the bioreactor system to modulate the COtransfer to and from the cell culture such that the pH of the cell culture can be maintained within a desired range.

Method Of Culturing Eukaryotic Cells
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US Patent:

20190233790, Aug 1, 2019
Filed:

Dec 12, 2018
Appl. No.:

16/218323
Inventors:

- South San Francisco CA, US
Jenny HSIUNG - Mountain View CA, US
Inn H. YUK - Berkeley CA, US
Assignee:

GENENTECH, INC. - South San Francisco CA
International Classification:

C12M 1/00
C12M 1/34
C12N 5/00
C12M 1/36
C12M 3/06
Abstract:

An apparatus and method to maintain pH within a range conducive for cell growth in a bicarbonate-containing cell culture system without the addition of base. The method relies on the gas transfer characteristics of the bioreactor system to modulate the COtransfer to and from the cell culture such that the pH of the cell culture can be maintained within a desired range.

Compositions And Methods For Detecting And Quantifying Host Cell Protein In Cell Lines And Recombinant Polypeptide Products
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US Patent:

20180364235, Dec 20, 2018
Filed:

Mar 7, 2018
Appl. No.:

15/914094
Inventors:

- South San Francisco CA, US
Yi-Chun Hsiao - San Mateo CA, US
Denise C. Krawitz - San Anselmo CA, US
Margaret S. Lin - Danville CA, US
Martin Vanderlaan - San Francisco CA, US
Rajesh Vij - San Mateo CA, US
Inn H. Yuk - Berkeley CA, US
Assignee:

Genentech, Inc. - South San Francisco CA
International Classification:

G01N 33/573
G01N 33/577
C07K 16/40
Abstract:

Monoclonal and polyclonal antibodies that bind hamster phospholipase B-like 2 are provided. Also provided are methods for detecting and quantifying hamster phospholipase B-like 2, for example, in recombinant polypeptide preparations, as well as kits for carrying out such methods. Methods of screening or selecting host cell lines or recombinant polypeptide-expressing cell lines that express low levels of hamster phospholipase B-like 2 are also provided.

Inn H Yuk