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Inn H Yuk

from Berkeley, CA

Inn Yuk Phones & Addresses

  • Berkeley, CA

Us Patents

  • Serum-Free Virus Propagation Platform For A Virus Vaccine Candidate

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  • US Patent:
    20110053248, Mar 3, 2011
  • Filed:
    Oct 5, 2007
  • Appl. No.:
    12/445950
  • Inventors:
    Inn Yuk - Berkeley CA, US
    Wenlin Zeng - Union City CA, US
    Misa Sugui - Santa Clara CA, US
  • Assignee:
    MEDIMMUNE, LLC - Gaithersburg MD
  • International Classification:
    C12N 7/00
    C12N 5/071
  • US Classification:
    4352351, 435364
  • Abstract:
    The invention relates to methods for propagating viruses. In particular, the invention provides optimized conditions for propagating viruses. Optimization of the following parameters are provided: lipid concentrates as supplements to the medium, temperature shift from pre-infection to post-infection, multiplicity of infection, direct bead-to-bead transfer and serum supplementation of pre-infection medium. In particular, the invention provides for the first time a method for propagating a virus by culturing cells that are infected with the virus in a medium comprising chemically defined lipid concentrate (CDLC). In another claim, the CDLC is added to medium that is substantially free of serum for culture of virus-infected cells.
  • Compositions And Methods For Regulating Cell Osmolarity

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  • US Patent:
    20110269233, Nov 3, 2011
  • Filed:
    Sep 15, 2009
  • Appl. No.:
    13/063912
  • Inventors:
    Laetitia Malphettes - San Francisco CA, US
    Andrew Snowden - Somerville MA, US
    Inn H. Yuk - Burlingame CA, US
  • International Classification:
    C12N 15/63
    C12N 5/10
    C12N 1/19
    C12N 1/21
    C12N 1/15
  • US Classification:
    435440, 4353201, 43525231, 43525233, 43525235, 43525234, 4352523, 4352543, 43525421, 43525423, 4352542, 435348, 435358, 435365, 435366, 435354
  • Abstract:
    The invention provides compositions and methods for regulating intracellular osmolarity in cells, e.g., in cultured cells, including in cultured cells in bioreactors. The invention provides nucleic acids comprising at least one osmo-responsive transcriptional regulatory element (OR-TRE), and cells, vectors, products of manufacture, artificial organs or implants and the like containing an osmo-responsive transcriptional regulatory element (OR-TRE).
  • Method Of Culturing Eukaryotic Cells

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  • US Patent:
    20120329151, Dec 27, 2012
  • Filed:
    Jul 6, 2010
  • Appl. No.:
    13/383829
  • Inventors:
    Dinesh Baskar - San Mateo CA, US
    Jenny Hsiung - Mountain View CA, US
    Inn H. Yuk - Berkeley CA, US
  • Assignee:
    F. HOFFMANN-LA ROCHE AG - Basel
  • International Classification:
    C12N 5/071
    C12M 3/00
  • US Classification:
    435350, 435325, 435363, 435366, 435351, 435352, 4352891
  • Abstract:
    An apparatus and method to maintain pH within a range conducive for cell growth in a bicarbonate-containing cell culture system without the addition of base. The method relies on the gas transfer characteristics of the bioreactor system to modulate the CO2 transfer to and from the cell culture such that the pH of the cell culture can be maintained within a desired range.
  • Methods Of Protein Purification

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  • US Patent:
    20100256336, Oct 7, 2010
  • Filed:
    Sep 17, 2007
  • Appl. No.:
    12/441775
  • Inventors:
    Inn Huam Yuk - Berkeley CA, US
    Bradley Richard Snedecor - Portola Valley CA, US
    Dana Christian Andersen - Menlo Park CA, US
  • International Classification:
    C07K 16/28
    C12P 21/02
    C12P 21/08
  • US Classification:
    5303873, 435 711, 530350, 5303871
  • Abstract:
    A method of reducing glycation of an amino acid in a protein produced by a host cell in cell culture medium is disclosed.
  • Modified Mammalian Cells

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  • US Patent:
    20210309989, Oct 7, 2021
  • Filed:
    Mar 26, 2021
  • Appl. No.:
    17/213611
  • Inventors:
    - South San Francisco CA, US
    Shahram MISAGHI - South San Francisco CA, US
    Amy SHEN - South San Francisco CA, US
    Anthony TOMLINSON - South San Francisco CA, US
    Inn Huam YUK - South San Francisco CA, US
  • Assignee:
    GENENTECH, INC. - South San Francisco CA
  • International Classification:
    C12N 15/10
    C12N 5/071
  • Abstract:
    The present disclosure relates to methods, cells, and compositions for producing a product of interest, e.g., a recombinant protein. In particular, the present disclosure provides improved mammalian cells expressing the product of interest, where the cells (e.g., Chinese Hamster Ovary (CHO) cells) have reduced or eliminated activity, e.g., expression, of certain host cell proteins, e.g., enzymes including, but not limited to, certain lipases, esterases, and/or hydrolases.
  • Method Of Culturing Eukaryotic Cells

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  • US Patent:
    20210130759, May 6, 2021
  • Filed:
    Jan 13, 2021
  • Appl. No.:
    17/148190
  • Inventors:
    - South San Francisco CA, US
    Jenny HSIUNG - Mountain View CA, US
    Inn H. YUK - Berkeley CA, US
  • Assignee:
    Genentech, Inc. - South San Francisco CA
  • International Classification:
    C12M 1/00
    C12M 3/06
    C12M 1/34
    C12M 1/36
    C12N 5/00
  • Abstract:
    An apparatus and method to maintain pH within a range conducive for cell growth in a bicarbonate-containing cell culture system without the addition of base. The method relies on the gas transfer characteristics of the bioreactor system to modulate the COtransfer to and from the cell culture such that the pH of the cell culture can be maintained within a desired range.
  • Method Of Culturing Eukaryotic Cells

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  • US Patent:
    20190233790, Aug 1, 2019
  • Filed:
    Dec 12, 2018
  • Appl. No.:
    16/218323
  • Inventors:
    - South San Francisco CA, US
    Jenny HSIUNG - Mountain View CA, US
    Inn H. YUK - Berkeley CA, US
  • Assignee:
    GENENTECH, INC. - South San Francisco CA
  • International Classification:
    C12M 1/00
    C12M 1/34
    C12N 5/00
    C12M 1/36
    C12M 3/06
  • Abstract:
    An apparatus and method to maintain pH within a range conducive for cell growth in a bicarbonate-containing cell culture system without the addition of base. The method relies on the gas transfer characteristics of the bioreactor system to modulate the COtransfer to and from the cell culture such that the pH of the cell culture can be maintained within a desired range.
  • Compositions And Methods For Detecting And Quantifying Host Cell Protein In Cell Lines And Recombinant Polypeptide Products

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  • US Patent:
    20180364235, Dec 20, 2018
  • Filed:
    Mar 7, 2018
  • Appl. No.:
    15/914094
  • Inventors:
    - South San Francisco CA, US
    Yi-Chun Hsiao - San Mateo CA, US
    Denise C. Krawitz - San Anselmo CA, US
    Margaret S. Lin - Danville CA, US
    Martin Vanderlaan - San Francisco CA, US
    Rajesh Vij - San Mateo CA, US
    Inn H. Yuk - Berkeley CA, US
  • Assignee:
    Genentech, Inc. - South San Francisco CA
  • International Classification:
    G01N 33/573
    G01N 33/577
    C07K 16/40
  • Abstract:
    Monoclonal and polyclonal antibodies that bind hamster phospholipase B-like 2 are provided. Also provided are methods for detecting and quantifying hamster phospholipase B-like 2, for example, in recombinant polypeptide preparations, as well as kits for carrying out such methods. Methods of screening or selecting host cell lines or recombinant polypeptide-expressing cell lines that express low levels of hamster phospholipase B-like 2 are also provided.

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