Henry J Cattadoris

US Patent:

8058057, Nov 15, 2011

Filed:

Sep 30, 2008

Appl. No.:

12/241522

Inventors:

Henry J. Cattadoris - Scarborough ME, US
Martin J. Popoloski - Lowell MA, US
Allison J. Tanner - Portsmouth NH, US

Assignee:

Corning Incorporated - Corning NY

International Classification:

C12M 1/00
C12M 3/00

US Classification:

4352941, 4353042, 366275

Abstract:

A cell culture apparatus includes a housing, a bag and an inflatable bladder. The bag is disposed in the housing and has an interior surface defining a chamber for culturing cells. The bladder is disposed in the housing, external to the bag, and is sufficiently inflatable and expandable to exert pressure on the bag when the chamber is substantially free of fluid. The bladder may be inflated and expanded to reduce movement of the bag within the housing. Alternatively, or in addition, the bladder may be inflated and expanded to apply a mechanical stress on cells cultured within the chamber of the bag.

US Patent:

8177082, May 15, 2012

Filed:

Apr 18, 2008

Appl. No.:

12/148321

Inventors:

Henry Joseph Cattadoris - Scarborough ME, US
Gregory Roger Martin - Acton ME, US
Allison Jean Tanner - Portsmouth NH, US

Assignee:

Corning Incorporated - Corning NY

International Classification:

B65D 53/00
C12M 1/24
C12M 3/00

US Classification:

215270, 215248, 215261, 4352971, 4353031, 4353041, 4353043, 4353051, 4353054, 4353071, 251213, 251294, 251298, 251336

Abstract:

An assembly for culturing cells includes a housing defining a reservoir for containing cell culture media and an opening in fluid communication with the reservoir. The cell culture assembly further includes a filter valve assembly configured to sealingly engage the opening. The filter valve assembly includes a flexible membrane, a contiguous side wall, an end wall, and a microbial filter. The end wall has an opening and extends across one end of the side wall to form a major cavity within the side wall and the end wall. The flexible membrane has a slit spanning the membrane and is disposable within the major cavity such that the membrane sealingly divides the major cavity into (i) a chamber formed between the end wall and the membrane and (ii) a minor cavity formed by the membrane and a portion of the side wall. The minor cavity is in fluid communication with the reservoir. The slit is biased in a closed position to prevent fluid from passing from the minor cavity into the chamber and is configured to open to allow gas to pass from the minor cavity into the chamber when the pressure differential across the membrane is above a threshold.

US Patent:

8178345, May 15, 2012

Filed:

Sep 16, 2008

Appl. No.:

12/211378

Inventors:

Scott M. Bennett - Limerick ME, US
Henry J. Cattadoris - Scarborough ME, US
David A. Kenney - Lunenburg MA, US
Gregory R. Martin - Acton ME, US
Allison J. Tanner - Portsmouth NH, US
Joseph C. Wall - Southborough MA, US

Assignee:

Corning Incorporated - Corning NY

International Classification:

C12M 1/24
C12M 1/00
C12M 1/14

US Classification:

4353042, 4352891, 4352991, 4352992, 4353041, 4353043

Abstract:

A multilayered cell culture apparatus for the culturing of cells is disclosed. The cell culture apparatus includes a unitary flask body including a rigid upper and lower surface, connected by side walls. The cell growth apparatus comprises multiple cell growth chambers stacked in vertical alignment and orientation within the unitary flask body. The stacked chambers are held in position by unitary connecting columns that run through each cell growth chamber and terminate at the rigid upper and lower surfaces of the apparatus. The cell growth chambers are separated by tracheal spaces that allow air from the external environment to contact the cell growth surface of each individual cell growth chamber.

US Patent:

8216828, Jul 10, 2012

Filed:

Sep 16, 2008

Appl. No.:

12/211430

Inventors:

Henry J. Cattadoris - Scarborough ME, US
Gregory R. Martin - Acton ME, US
Allison J. Tanner - Portsmouth NH, US

Assignee:

Corning Incorporated - Corning NY

International Classification:

C12M 1/12
C12M 3/00
C12M 1/24
B29C 35/08
B29C 35/00

US Classification:

4352975, 4352941, 4352971, 4353042, 4353043, 264492, 264493, 264494

Abstract:

A cell culture apparatus includes a cell culture chamber formed by a first major surface, an opposing second major surface spaced apart from the first major surface, and a first side wall around the first chamber and extending between the first and second major surfaces. A portion of the first sidewall proximate the first major surface comprises an infrared absorbent material, and a portion the first sidewall proximate the second major surface is formed from substantially non-infrared absorbent material. The first major surface is formed by a gas permeable polymeric film that is impermeable to cell culture liquid.

US Patent:

20060019377, Jan 26, 2006

Filed:

Jul 22, 2005

Appl. No.:

11/187045

Inventors:

Henry Cattadoris - Old Orchard Beach ME, US

International Classification:

C12M 1/24

US Classification:

435304300

Abstract:

The present invention provides a flask having a top piece and a bottom piece with optical properties on the interior surface of which cells are grown. The top piece and the bottom piece are joined by a removable elastomeric seal . By disengaging the elastomeric seal from the flask body , the top piece can be separated from the bottom piece providing access to the flask interior.

US Patent:

20090263892, Oct 22, 2009

Filed:

Apr 18, 2008

Appl. No.:

12/148322

Inventors:

Henry Joseph Cattadoris - Scarborough ME, US
Gregory Roger Martin - Acton ME, US
Allison Jean Tanner - Portsmouth NH, US

International Classification:

C12M 1/00
F16K 27/00

US Classification:

4352891, 251366

Abstract:

A cell culture vessel includes a housing defining a reservoir, an inlet and an outlet in fluid communication with the reservoir, a valve assembly, and an antimicrobial filter. The valve assembly includes (i) a side wall defining a passageway for fluid flow between the reservoir and the outlet, (ii) a floating element disposed in the passageway and configured to float on the culture medium, (iii) a stop feature configured to sealingly engage the floating element to prevent the culture medium from flowing from the reservoir through the outlet, and (iv) a capture feature configured to retain the floating element within the passageway and to allow fluid to flow from the reservoir through the passageway. The microbial filter is positioned such that air flowing in the outlet to the passageway passes through the filter.