Gary E Siuzdak

US Patent:

7632685, Dec 15, 2009

Filed:

Dec 28, 2006

Appl. No.:

11/647689

Inventors:

Richard M. Ivey - Parkton MD, US
Richard L. Moore - Glenville PA, US
Michael L. Towns - Timonium MD, US
Nicholas Bachur, Jr. - Monkton MD, US
Robert W. Rosenstein - Ellicott City MD, US
James G. Nadeau - Ellicott City MD, US
Paul E. Goldenbaum - Hampstead MD, US
Song Shi - Reisterstown MD, US
Donald Copertino - Catonsville MD, US
James Garrett - Baltimore MD, US
Gregory Tice - Lutherville MD, US
Gary Siuzdak - San Diego CA, US
Elizabeth Want - San Diego CA, US
Zhouxin Shen - San Diego CA, US

Assignee:

Becton, Dickinson and Company - Franklin Lake NJ

International Classification:

G01N 24/00

US Classification:

436173, 436 89

Abstract:

Mass spectrometry techniques for determining the status of sepsis in an individual are provided. A biomarker profile resolved from a biological sample, taken from the individual, using a mass spectrometry technique is compared to a reference biomarker profile. A single such comparison classifies the individual as belonging to or not belonging to a reference population. The individual's biomarker profile and the reference biomarker profile comprise a plurality of ions each having a mass-to-charge ratio of about 100 Daltons to about 1000 Daltons. The plurality of ions can be detected by electrospray ionization mass spectrometry in positive mode. The comparison uses a decision rule, such as a classification tree, that determines the status of sepsis in the individual without requiring knowledge of the identity of the biomarkers in the biomarker profile from the individual and without requiring knowledge of the identity of the biomarkers in the reference biomarker profile.

US Patent:

7645613, Jan 12, 2010

Filed:

Dec 28, 2006

Appl. No.:

11/647688

Inventors:

Richard M. Ivey - Parkton MD, US
Richard L. Moore - Glenville PA, US
Michael L. Towns - Timonium MD, US
Gary Siuzdak - San Diego CA, US
Elizabeth J. Want - San Diego CA, US
Zhouxin Shen - San Diego CA, US
Nicholas Bachur, Jr. - Monkton MD, US
Robert W. Rosenstein - Ellicott City MD, US
James G. Nadeau - Ellicott City MD, US
Paul E. Goldenbaum - Hampstead MD, US
Song Shi - Reisterstown MD, US
Donald Copertino - Catonsville MD, US
James Garrett - Baltimore MD, US
Gregory Tice - Lutherville MD, US

Assignee:

Becton, Dickinson and Company - Franklin Lakes NJ

International Classification:

G01N 24/00

US Classification:

436173, 436 89

Abstract:

Mass spectrometry techniques for determining the status of sepsis in an individual are provided. A biomarker profile resolved from a biological sample, taken from the individual, using a mass spectrometry technique is compared to a reference biomarker profile. A single such comparison classifies the individual as belonging to or not belonging to a reference population. The individual's biomarker profile and the reference biomarker profile comprise a plurality of ions each having a mass-to-charge ratio of about 100 Daltons to about 1000 Daltons. The plurality of ions can be detected by electrospray ionization mass spectrometry in positive mode. The comparison uses a decision rule, such as a classification tree, that determines the status of sepsis in the individual without requiring knowledge of the identity of the biomarkers in the biomarker profile from the individual and without requiring knowledge of the identity of the biomarkers in the reference biomarker profile.

US Patent:

20030057106, Mar 27, 2003

Filed:

Sep 12, 2002

Appl. No.:

10/242018

Inventors:

Zhouxin Shen - San Diego CA, US
Gary Siuzdak - San Diego CA, US

International Classification:

B23H003/00
C25F003/00
H05K003/07
B23H005/00

US Classification:

205/655000, 435/002000, 436/177000, 436/178000

Abstract:

A method of making improved substrates for desorbing and ionizing analytes takes an n-type semiconductor substrate and provides a strong light source. By focusing the illumination from the light source onto the n-type semiconductor substrate results in at least one lit region on the n-type semiconductor substrate. The substrate is electrochemically etched with a low current during illumination to form at least one sample reservoir, each sample reservoir being formed at a respective lit region on the n-type semiconductor substrate.

US Patent:

20040157242, Aug 12, 2004

Filed:

Nov 12, 2003

Appl. No.:

10/704758

Inventors:

Richard Ivey - Parkton MD, US
Thomas Gentle - Red Lion PA, US
Richard Moore - Glenville PA, US
Michael Towns - Timonium MD, US
Nicholas Bachur - Monkton MD, US
Robert Rosenstein - Ellicott City MD, US
James Nadeau - Ellicott City MD, US
Paul Goldenbaum - Hampstead MD, US
Song Shi - Reisterstown MD, US
Donald Copertino - Catonsville MD, US
James Garrett - Baltimore MD, US
Gregory Tice - Lutherville MD, US
Gary Siuzdak - San Diego CA, US
Elizabeth Want - San Diego CA, US
Zhouxin Shen - San Diego CA, US

Assignee:

Becton, Dickinson and Company - Franklin Lakes NJ

International Classification:

C12Q001/68
G01N033/53

US Classification:

435/006000, 435/007100

Abstract:

The early prediction or diagnosis of sepsis advantageously allows for clinical intervention before the disease rapidly progresses beyond initial stages to the more severe stages, such as severe sepsis or septic shock, which are associated with high mortality. Early prediction or diagnosis is accomplished by comparing an individual's profile of biomarker expression to profiles obtained from one or more control, or reference, populations, which may include a population who develops sepsis. Recognition of features in the individual's biomarker profile that are characteristic of the onset of sepsis allows a clinician to diagnose the onset of sepsis from a bodily fluid isolated at the individual at a single point in time. The necessity of monitoring the patient over a period of time is, therefore, avoided, advantageously allowing clinical intervention before the onset of serious symptoms. Further, because the biomarker expression is assayed for its profile, identification of the particular biomarkers is unnecessary. The comparison of an individual's biomarker profile to biomarker profiles of appropriate reference populations likewise can be used to diagnose SIRS in the individual.

US Patent:

20060157647, Jul 20, 2006

Filed:

Jan 18, 2005

Appl. No.:

11/036160

Inventors:

Gary Siuzdak - San Diego CA, US
Zhouxin Shen - San Diego CA, US

Assignee:

Becton, Dickinson and Company - Franklin Lakes NJ

International Classification:

H01J 49/04
G01N 30/72
G01N 30/62

US Classification:

250288000, 324307000, 210656000

Abstract:

A liquid chromatography configuration providing at least three dimensions of separation coupled with spectrometry greatly improves the ability to detect ions present in samples, including complex biological samples such as blood. Liquid chromatography columns in one embodiment are connected with an in-line trapping column the alternately communicates with the second and third liquid chromatography columns. The liquid chromatography columns are operably connected either to a mass spectrometer (MS) or a nuclear magnetic resonance (NMR) spectrometer. The improved dynamic range of detection allows a method of detecting molecular components present in complex biological samples that serve as biomarkers for a disease state, such as sepsis.

US Patent:

20060157648, Jul 20, 2006

Filed:

Nov 29, 2005

Appl. No.:

11/288590

Inventors:

Gary Siuzdak - San Diego CA, US
Eden Go - San Diego CA, US
Zhouxin Shen - San Diego CA, US
Bruce Compton - Lexington MA, US
Edouard Bouvier - Stow MA, US
Grace Credo - Foster City CA, US

Assignee:

WATERS INVESTMENTS LIMITED - New Castle DE

International Classification:

H01J 49/00

US Classification:

250288000

Abstract:

Embodiments of the present invention are directed to a substrate for performing ionization desorption on porous silicon, methods for performing such ionization desorption and methods of making substrates. One embodiment directed to a substrate for performing ionization desorption on silicon comprises a substrate having a surface having a formula of: As used above, X is H or Y, where at least at least twenty five mole percent of X is Y and Y is hydroxyl, or —O—Ror O—SiR,R,Rwherein R,R, and Rare selected from the group consisting Cto Cstraight, cyclic, or branched alkyl, aryl, or alkoxy group, a hydroxyl group, or a siloxane group, and Rmay be a Cto Cstraight, cyclic, or branched alkyl (e.g., Ccyanopropyl), aryl, or alkoxy group, where the groups of Rare unsubstituted or substituted with one or more moieties such as halogen, cyano, amino, diol, nitro, ether, carbonyl, epoxide, sulfonyl, cation exchanger, anion exchanger, carbamate, amide, urea, peptide, protein, carbohydrate, and nucleic acid functionalities. The letter “n” represents an integer from 1 to infinity and any vacant valences are silicon atoms, hydrogen or impurities.

US Patent:

20080073512, Mar 27, 2008

Filed:

Jul 27, 2007

Appl. No.:

11/829170

Inventors:

Gary Siuzdak - San Diego CA, US
Eden Go - San Diego CA, US
Zhouxin Shen - San Diego CA, US
Bruce Compton - Lexington MA, US
Edouard Bouvier - Stow MA, US
Grace Credo - Foster City CA, US

Assignee:

WATERS INVESTMENTS LIMITED - New Castle DE

International Classification:

C01B 33/113
C07F 7/02
H01J 49/16

US Classification:

250288000, 423325000, 556400000

Abstract:

Embodiments of the present invention are directed to a substrate for performing ionization desorption on porous silicon, methods for performing such ionization desorption and methods of making substrates. One embodiment directed to a substrate for performing ionization desorption on silicon comprises a substrate having a surface having a formula of: As used above, X is H or Y, where at least at least twenty five mole percent of X is Y and Y is hydroxyl, or —O—R, or O—SiR, R, Rwherein R, R, and Rare selected from the group consisting of alkyl, alkenyl, alkynyl, aromatic, amino alkyl, amino alkenyl, amino alkynyl, pyridinyl, pyrridonyl, and carbonyl, alcohol and carboxylic acid derivatives thereof having one to twenty five atoms, and hydroxyl, amino, amide, carboxyl, ester, carbonyl, sulfhydryl, sulfonyl, phosphoral, bromo, iodo, chloro and fluoro derivatives. The letter “n” represents an integer from 1 to infinity and any vacant valences are silicon atoms, hydrogen or impurities.

US Patent:

20080128608, Jun 5, 2008

Filed:

Sep 10, 2007

Appl. No.:

11/852863

Inventors:

Trent Northen - San Diego CA, US
Gary Siuzdak - San Diego CA, US
Anders Nordstrom - Darneryd, SE

Assignee:

The Scripps Research Institute - LaJolla CA

International Classification:

H01J 49/00
B32B 3/00
B32B 3/26

US Classification:

250282, 250288, 428156, 4283044

Abstract:

A substrate for use in providing an ionized target comprising a structured substrate has a plurality of recesses, at least a portion of the plurality of recesses containing an initiator, the substrate being capable of having a target loaded on it. In one methods, irradiation of the substrate can cause the initiator to restructure, releasing it from the recesses and thereby desorbing and ionizing the target. The target so desorbed and ionized can be detected by mass analyzers. The mass of the targets at a given point on the surface can be recorded to provide a spatial mapping of the targets on the surface.