Eric M Priuska

US Patent:

20050181432, Aug 18, 2005

Filed:

Mar 30, 2005

Appl. No.:

11/095971

Inventors:

Eric Priuska - Ann Arbor MI, US
Richard Smith - Ann Arbor MI, US
Judith Erb - Ann Arbor MI, US
James Downward - Ann Arbor MI, US
Otho Ulrich - Ann Arbor MI, US

International Classification:

C12Q001/68
G01N033/53

US Classification:

435006000, 435007100

Abstract:

A method for performing a rapid, homogenous assays for monitoring the reactions of a binding target, by immobilizing a fluorescent-capable chelate complex that is derivatized so as to posses recognition binding ligands, labeling the complex with a labeled second chelator that is added to the assay thereby forming a fluorescent mixed chelate, and measuring the fluorescent mixed chelate, whereby the measurement of the label enable monitoring of the reaction of the binding target. A rapid assay for performing the above method including a first chelating molecule, a fluorescent-capable ion complexed with the first chelating molecule, a second chelating molecule for reacting with the fluorescent-capable ion complexed with the first chelating molecule, and a measuring device for measuring fluorescent resulting from the second chelating molecule reacting with the fluorescent-capable ion complexed with the first chelating molecule. A biosensor for monitoring molecular interactions between receptors, including a biosensor having attached thereto a fluorescent-capable ion complexed with a first chelating molecule, whereby upon exposure to a second chelating molecule said complex becomes fluorescent is also provided.

US Patent:

20030008314, Jan 9, 2003

Filed:

Jun 28, 2002

Appl. No.:

10/186151

Inventors:

Eric Priuska - Ann Arbor MI, US
Richard Smith - Ann Arbor MI, US
Judith Erb - Ann Arbor MI, US
James Downward - Ann Arbor MI, US
Otho Ulrich - Ann Arbor MI, US

International Classification:

C12Q001/68
G01N033/53
G01N033/542

US Classification:

435/006000, 435/007900

Abstract:

A method for performing a rapid, homogenous assays for monitoring the reactions of a binding target, by immobilizing a fluorescent-capable chelate complex that is derivatized so as to posses recognition binding ligands, labeling the complex with a labeled second chelator that is added to the assay thereby forming a fluorescent mixed chelate, and measuring the fluorescent mixed chelate, whereby the measurement of the label enable monitoring of the reaction of the binding target. A rapid assay for performing the above method including a first chelating molecule, a fluorescent-capable ion complexed with the first chelating molecule, a second chelating molecule for reacting with the fluorescent-capable ion complexed with the first chelating molecule, and a measuring device for measuring fluorescent resulting from the second is chelating molecule reacting with the fluorescent-capable ion complexed with the first chelating molecule. A biosensor for monitoring molecular interactions between receptors, including a biosensor having attached thereto a fluorescent-capable ion complexed with a first chelating molecule, whereby upon exposure to a second chelating molecule said complex becomes fluorescent is also provided.