Daryl S Ogden

US Patent:

49593080, Sep 25, 1990

Filed:

Aug 27, 1987

Appl. No.:

7/089884

Inventors:

Daryl M. Ogden - Houston TX

Assignee:

Board of Regents, The University of Texas System - Austin TX

International Classification:

C07K 1700
G01N 33543

US Classification:

435 7

Abstract:

The present invention involves a method for producing a substrate useful in a system for the detection of antibodies directed against platelet antigens. This method comprises several steps. A platelet sample of interest is initially treated with an aqueous solution comprising a dialyzable nonionic detergent. This initial treatment is under conditions to solubilize platelet components and produce a platelet lysate. Such conditions may involve treatment of a platelet sample with an aqueous solution comprising nonionic detergent at a concentration between about 0. 2% and about 0. 5%. Platelet antigens are most preferably solubilized for about 30 min and at about 0. degree. C. in an aqueous solution comprising about 1 mg dialyzable nonionic detergent per mg platelet protein. The partially purified platelet antigens resulting from these manipulations are then preferably affixed to a solid matrix. The solid matrix preferably comprises nitrocellulose paper, polystyrene or latex but may be any solid matrix suitable for the abstraction from a biological sample and/or assay of antibodies binding to the affixed purified platelet antigens.