Cindy Peiyee Kan

US Patent:

20100081786, Apr 1, 2010

Filed:

Apr 11, 2007

Appl. No.:

12/296608

Inventors:

Samuel J. Danishefsky - Englewood NJ, US
J. David Warren - Lodi NJ, US
Jiehao Chen - Fishers IN, US
Bin Wu - Thousand Oaks CA, US
Gong Chen - New York NY, US
Qian Wan - New York NY, US
Zhongping Tan - New York NY, US
Cindy Kan - New York NY, US
Yu Yuan - New York NY, US
Zihao Hua - New York NY, US
Krishnakumar Ranganathan - Oak Park CA, US
John D. Trzupek - New York NY, US

International Classification:

C07K 14/505
C07K 1/00

US Classification:

530322, 530333, 530345

Abstract:

The present invention provides isolated homogeneous polyfunctionalized proteins (e.g., erythropoietin), isolated glycopeptides, and a method for preparing polyfunctionalized peptides and/or proteins via cysteine-free native chemical ligation. In certain embodiments, the invention provides an isolated homogeneous polyfunctionalized protein having the structure (I). In certain other embodiments, the invention provides an isolated glycopeptide having Formula (II). In certain other embodiments, the inventive method is a method for preparing a polyfunctionalized peptide comprising a peptidic backbone made up of four or more amino acids, wherein two or more non-adjacent amino acids are independently substituted with a moiety having the structure (III)-LH. wherein A and L1 are as defined herein.