A-Star 2011 - 2015
Associate Director
Harvard Medical School Aug 1993 - 2015
Associate Professor of Medicine
Beth Israel Deaconess Medical Center 1988 - 2014
Associate Physician
Merck 1988 - 2014
Associate Vice President For Research Science and Translational Medicine
A-Star 1988 - 2014
Senior Group Leader
Education:
University of Toronto 1985
Doctorates, Doctor of Philosophy, Molecular Biology, Philosophy
Harvard Medical School
Western University
Doctor of Medicine, Doctorates, Medicine
Skills:
Genomics Research Strategic Alliances Cross Functional Collaborations Pediatric Hematology/Oncology Drug Discovery Team Management Translational Research Embryonic Stem Cells Cancer Stem Cells Oncology Hematopoiesis Functional Genomics
The identification of 101 genes upregulated or differentially expressed by mature human oocytes is provided herein. These genes and the corresponding gene products will facilitate a greater understanding of oogenesis, folliculogenesis, fertilization, and embryonic development. In addition these genes and the corresponding gene products can be used to effect dedifferentiation and/or transdifferentiation of desired somatic cells. The resultant dedifferentiated cells and somatic cells derived therefrom can be used in cell therapies such as in the treatment of cancer, autoimmunity, and other diseases wherein specific types of cells such as hematopoietic cells may be depleted because of the underlying disease or the treatment of the disease.Also, a core group of 66 transcripts was identified by intersecting significantly up-regulated genes of the human oocyte with those from the mouse oocyte and from human and mouse embryonic stem cells. Within the up-regulated probe sets, the top overrepresented categories were related to RNA and protein metabolism, followed by DNA metabolism and chromatin modification. This invention therefore provides a comprehensive expression baseline of genes expressed in in vivo matured human oocytes. Further understanding of the biological role of these genes will also expand knowledge on meiotic cell cycle, fertilization, chromatin remodeling, lineage commitment, pluripotency, tissue regeneration, and morphogenesis.
Bing Lim - Dorchester MA Jean-Michel Lelias - Evry, FR Chaker N. Adra - Boston MA Jone L. Ko - Sudbury MA
Assignee:
Beth Israel Hospital Association - Boston MA
International Classification:
A61K 3817 C07K 1447 C12N 506 C12N 508
US Classification:
514 12
Abstract:
The sequence, molecular structure and expression of a cDNA clone, denoted D4, of human and murine origin, preferentially expressed in hematopoietic cells is described herein. The human cDNA clone has been expressed in bacteria and the predicted 24 Kd protein purified. The protein has been used in studies of its biochemical function. As predicted on the basis of sequence, D4 can function as a GDP-dissociation inhibitor of at least several small GTP-binding proteins (CDC42 and rac). The D4 protein was used to generate a polyclonal antibody specific for the protein. The human cDNA was used to obtain several full length murine genomic clones. A clone has been analyzed and sequenced to use for the construction of a gene-targeting vector to produce animals deficient in D4 through disruption of the gene by homologous recombination. These animals can then be used as models for fundamental and applied research on the GTP-binding proteins.
Bing Lim - Dorchester MA Chaker N. Adra - Boston MA Jean-Michel Lelias - Columbus OH
Assignee:
Beth Israel Deaconess Medical Center - Boston MA
International Classification:
C07K 1628
US Classification:
5303879
Abstract:
The invention relates to a recombinant DNA molecule which encodes a HT. sub. m4 protein, a transformed host cell which has been stably transfected with a DNA molecule which encodes a HT. sub. m4 protein and a recombinant HT. sub. m4 protein. The invention also relates to a method for detecting the presence of a hereditary atopy.
Lysosomal-Associated Multispanning Membrane Protein, Laptm5 And Nucleic Acid Encoding Laptm5
Bing Lim - Dorchester MA Chaker N. Adra - Boston MA
International Classification:
C12N 1512
US Classification:
435 691
Abstract:
This invention relates to the discovery of a novel nucleic acid molecule which encodes a hematopoietic-specific protein, termed LAPTM5. The expression pattern of the gene together with evidence that the protein interacts with ubiquitin indicates that the protein has a functional role during embryogenesis and in adult hematopoietic cells.
Ht.sub.m4 Methods Of Treatment And Assays, Agonists And Antagonists
Bing Lim - Dorchester MA Chaker N. Adra - Boston MA Jean-Michel Lelias - Columbus OH
Assignee:
Beth Israel Deaconess Medical Center - Boston MA
International Classification:
C12N 1500 C12N 120 C07H 2104
US Classification:
4352523
Abstract:
The invention relates to a recombinant DNA molecule which encodes a HT. sub. m4 protein, a transformed host cell which has been stably transfected with a DNA molecule which encodes a HT. sub. m4 protein and a recombinant HT. sub. m4 protein. The invention also relates to a method for detecting the presence of a hereditary atopy.
Bing Lim - Dorchester MA Jean-Michel Lelias - Evry, FR Chaker N. Adra - Boston MA Jone L. Ko - Sudbury MA
Assignee:
Beth Israel Hospital Association - Boston MA
International Classification:
C12N 1511 C12N 1512 C12Q 168
US Classification:
536 235
Abstract:
The sequence, molecular structure and expression of a cDNA clone, denoted D4, of human and murine origin, preferentially expressed in hematopoietic cells is described herein. The human cDNA clone has been expressed in bacteria and the predicted 24 Kd protein purified. The protein has been used in studies of its biochemical function. As predicted on the basis of sequence, D4 can function as a GDP-dissociation inhibitor of at least several small GTP-binding proteins (CDC42 and rac). The D4 protein was used to generate a polyclonal antibody specific for the protein. The human cDNA was used to obtain several full length murine genomic clones. A clone has been analyzed and sequenced to use for the construction of a gene-targeting vector to produce animals deficient in D4 through disruption of the gene by homologous recombination. These animals can then be used as models for fundamental and applied research on the GTP-binding proteins.