Bing Lim

US Patent:

20090028835, Jan 29, 2009

Filed:

Sep 10, 2007

Appl. No.:

11/852677

Inventors:

Jose Bernardo Cibelli - East Lansing MI, US
Arif Murat Kocabas - East Lansing MI, US
Bing Lim - Boston MA, US
Hasan H. Otu - Istanbul, TR

Assignee:

Michigan State University - East Lansing MI

International Classification:

A61K 48/00
C12N 15/11
C12N 5/10
A61P 35/00
A61P 37/00
A61P 31/00
C12Q 1/68

US Classification:

424 9321, 536 231, 435366, 435455, 435 6

Abstract:

The identification of 101 genes upregulated or differentially expressed by mature human oocytes is provided herein. These genes and the corresponding gene products will facilitate a greater understanding of oogenesis, folliculogenesis, fertilization, and embryonic development. In addition these genes and the corresponding gene products can be used to effect dedifferentiation and/or transdifferentiation of desired somatic cells. The resultant dedifferentiated cells and somatic cells derived therefrom can be used in cell therapies such as in the treatment of cancer, autoimmunity, and other diseases wherein specific types of cells such as hematopoietic cells may be depleted because of the underlying disease or the treatment of the disease.Also, a core group of 66 transcripts was identified by intersecting significantly up-regulated genes of the human oocyte with those from the mouse oocyte and from human and mouse embryonic stem cells. Within the up-regulated probe sets, the top overrepresented categories were related to RNA and protein metabolism, followed by DNA metabolism and chromatin modification. This invention therefore provides a comprehensive expression baseline of genes expressed in in vivo matured human oocytes. Further understanding of the biological role of these genes will also expand knowledge on meiotic cell cycle, fertilization, chromatin remodeling, lineage commitment, pluripotency, tissue regeneration, and morphogenesis.

US Patent:

57670730, Jun 16, 1998

Filed:

Jun 1, 1994

Appl. No.:

8/252073

Inventors:

Bing Lim - Dorchester MA
Jean-Michel Lelias - Evry, FR
Chaker N. Adra - Boston MA
Jone L. Ko - Sudbury MA

Assignee:

Beth Israel Hospital Association - Boston MA

International Classification:

A61K 3817
C07K 1447
C12N 506
C12N 508

US Classification:

514 12

Abstract:

The sequence, molecular structure and expression of a cDNA clone, denoted D4, of human and murine origin, preferentially expressed in hematopoietic cells is described herein. The human cDNA clone has been expressed in bacteria and the predicted 24 Kd protein purified. The protein has been used in studies of its biochemical function. As predicted on the basis of sequence, D4 can function as a GDP-dissociation inhibitor of at least several small GTP-binding proteins (CDC42 and rac). The D4 protein was used to generate a polyclonal antibody specific for the protein. The human cDNA was used to obtain several full length murine genomic clones. A clone has been analyzed and sequenced to use for the construction of a gene-targeting vector to produce animals deficient in D4 through disruption of the gene by homologous recombination. These animals can then be used as models for fundamental and applied research on the GTP-binding proteins.

US Patent:

57056153, Jan 6, 1998

Filed:

Sep 3, 1996

Appl. No.:

8/707340

Inventors:

Bing Lim - Dorchester MA
Chaker N. Adra - Boston MA
Jean-Michel Lelias - Columbus OH

Assignee:

Beth Israel Deaconess Medical Center - Boston MA

International Classification:

C07K 1628

US Classification:

5303879

Abstract:

The invention relates to a recombinant DNA molecule which encodes a HT. sub. m4 protein, a transformed host cell which has been stably transfected with a DNA molecule which encodes a HT. sub. m4 protein and a recombinant HT. sub. m4 protein. The invention also relates to a method for detecting the presence of a hereditary atopy.

US Patent:

6153403, Nov 28, 2000

Filed:

Jul 8, 1997

Appl. No.:

8/889425

Inventors:

Bing Lim - Dorchester MA
Chaker N. Adra - Boston MA

International Classification:

C12N 1512

US Classification:

435 691

Abstract:

This invention relates to the discovery of a novel nucleic acid molecule which encodes a hematopoietic-specific protein, termed LAPTM5. The expression pattern of the gene together with evidence that the protein interacts with ubiquitin indicates that the protein has a functional role during embryogenesis and in adult hematopoietic cells.

US Patent:

59726881, Oct 26, 1999

Filed:

Dec 19, 1997

Appl. No.:

8/994578

Inventors:

Bing Lim - Dorchester MA
Chaker N. Adra - Boston MA
Jean-Michel Lelias - Columbus OH

Assignee:

Beth Israel Deaconess Medical Center - Boston MA

International Classification:

C12N 1500
C12N 120
C07H 2104

US Classification:

4352523

Abstract:

The invention relates to a recombinant DNA molecule which encodes a HT. sub. m4 protein, a transformed host cell which has been stably transfected with a DNA molecule which encodes a HT. sub. m4 protein and a recombinant HT. sub. m4 protein. The invention also relates to a method for detecting the presence of a hereditary atopy.

US Patent:

55854788, Dec 17, 1996

Filed:

Aug 18, 1994

Appl. No.:

8/292945

Inventors:

Bing Lim - Dorchester MA
Jean-Michel Lelias - Evry, FR
Chaker N. Adra - Boston MA
Jone L. Ko - Sudbury MA

Assignee:

Beth Israel Hospital Association - Boston MA

International Classification:

C12N 1511
C12N 1512
C12Q 168

US Classification:

536 235

Abstract:

The sequence, molecular structure and expression of a cDNA clone, denoted D4, of human and murine origin, preferentially expressed in hematopoietic cells is described herein. The human cDNA clone has been expressed in bacteria and the predicted 24 Kd protein purified. The protein has been used in studies of its biochemical function. As predicted on the basis of sequence, D4 can function as a GDP-dissociation inhibitor of at least several small GTP-binding proteins (CDC42 and rac). The D4 protein was used to generate a polyclonal antibody specific for the protein. The human cDNA was used to obtain several full length murine genomic clones. A clone has been analyzed and sequenced to use for the construction of a gene-targeting vector to produce animals deficient in D4 through disruption of the gene by homologous recombination. These animals can then be used as models for fundamental and applied research on the GTP-binding proteins.