Axel T Brunger

US Patent:

20060258846, Nov 16, 2006

Filed:

Mar 29, 2006

Appl. No.:

11/394392

Inventors:

Mark Breidenbach - San Francisco CA, US
Axel Brunger - San Francisco CA, US

International Classification:

C07K 14/33
G06F 19/00

US Classification:

530350000, 702019000

Abstract:

The present invention provides a crystallized form of the E224Q Y336F mutant of the light chain of botulinum neurotoxin serotype A (BoNT/A) protein as well as the atomic structure of this mutant protein. The present invention also provides crystals of the E224 Y336F mutant of the BoNT/A light chain in complex with the SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) domain of the SNAP-25 (synaptosome-associated protein-25 kDa) protein as well as the atomic structure of this complex. The present invention further provides a method of determining whether an agent mimics a region of the SNAP-25 protein that interacts with BoNT/A. With this method, a three-dimensional representation of the SNAP-25 region is used to computationally compare the agent with the three-dimensional representation of the SNAP-25 region.

US Patent:

20220403449, Dec 22, 2022

Filed:

Nov 29, 2021

Appl. No.:

17/537290

Inventors:

- Stanford CA, US
Stephen R. Quake - Stanford CA, US
Axel Brunger - Stanford CA, US
Jiajie Diao - Stanford CA, US

Assignee:

THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY - Stanford CA

International Classification:

C12Q 1/6818
C12Q 1/6834

Abstract:

Provided herein is a method for analyzing genomic DNA In some embodiments, the method may comprise labeling a genomic sample by adding a capture tag to the ends of the DNA molecules in the sample and labeling molecules that comprise hydroxymethylcytosine with a first fluorophore, immobilizing the labeled DNA molecules on a support, and imaging individual molecules of hydroxymethylated genomic DNA on the support.

US Patent:

20170298422, Oct 19, 2017

Filed:

Mar 27, 2017

Appl. No.:

15/470635

Inventors:

- Stanford CA, US
Stephen R. Quake - Stanford CA, US
Axel Brunger - Stanford CA, US
Jiajie Diao - Stanford CA, US

International Classification:

C12Q 1/68

Abstract:

Provided herein is a method for analyzing genomic DNA. In some embodiments, the method may comprise labeling a genomic sample by adding a capture tag to the ends of the DNA molecules in the sample and labeling molecules that comprise hydroxymethylcytosine with a first fluorophore, immobilizing the labeled DNA molecules on a support, and imaging individual molecules of hydroxymethylated genomic DNA on the support.