Ashutosh Chilkoti - Durham NC, US Angus Hucknall - Durham NC, US
Assignee:
Duke University - Durham NC
International Classification:
C40B 30/04 C40B 40/08 C40B 40/10 C40B 50/18
US Classification:
506 9, 506 17, 506 18, 506 32
Abstract:
An article such as a biomolecular detector or biosensor having a nonfouling surface thereon includes:(a) a substrate having a surface portion; (b) a linking layer on the surface portion; and (c) a polymer layer formed on the linking layer; and (d) a first member of a specific binding pair (e.g., a protein, peptide, antibody, nucleic acid, etc.) bound to the polymer layer. Methods of making and using the articles are also described.
- Durham NC, US Jacob Heggestad - Durham NC, US David Kinnamon - Durham NC, US Angus Hucknall - Durham NC, US
International Classification:
G01N 33/569 G01N 21/64
Abstract:
Disclosed herein are devices that can be used to detect neutralizing antibodies against multiple pathogens in a quick and accurate manner. An example device includes a substrate; a non-fouling layer; a plurality of pathogen regions, each pathogen region including a different pathogen; and at least one detection region, the detection region including a detection agent that is capable of specifically binding each pathogen and an excipient. In addition, an example method includes contacting a biological sample with a device, and detecting the presence of a neutralizing antibody in the biological sample for each pathogen, wherein the presence of the neutralizing antibody is detected by inhibiting the binding of the detection agent to each pathogen.
Detection And Assay Devices And Methods Of Making And Using The Same
An article such as a biomolecular detector or biosensor having a nonfouling surface thereon includes:(a) a substrate having a surface portion; (b) a linking layer on the surface portion; and (c) a polymer layer formed on the linking layer; and (d) a first member of a specific binding pair (e.g., a protein, peptide, antibody, nucleic acid, etc.) bound to the polymer layer. Methods of making and using the articles are also described.
- Durham NC, US Aaron Franklin - Durham NC, US Benjamin Yellen - Durham NC, US Angus Hucknall - Durham NC, US Daniel Joh - Durham NC, US Joseph Andrews - Durham NC, US
International Classification:
G01N 27/22 G01N 33/543 G01N 27/12
Abstract:
Disclosed are sensors that include a carbon nanotube channel and a non-fouling polymer layer, where the non-fouling polymer layer and the carbon nanotube channel do not directly contact each other and are separated by a dielectric layer. The disclosed sensors may be used, e.g., as biosensors for the accurate and sensitive detection of analytes within a sample. Also disclosed are methods of making and using the sensors.
An article such as a biomolecular detector or biosensor having a nonfouling surface thereon includes: (a) a substrate having a surface portion; (b) a linking layer on the surface portion; and (c) a polymer layer formed on the linking layer; and (d) a first member of a specific binding pair (e.g., a protein, peptide, antibody, nucleic acid, etc.) bound to the polymer layer. Methods of making and using the articles are also described.
Chips, Detectors, And Methods Of Making And Using The Same
Disclosed herein are chips, devices, methods of making the same, and methods of detecting a target analyte, and methods of diagnosing an individual with a disease or condition when a target analyte associated with the disease or condition is detected.