Amit Nathwani - London, GB Natalie Ward - London, GB Adrian Thrasher - London, GB Edward Tuddenham - London, GB John McVey - Beaconsfield, GB John Gray - Memphis TN, US Andrew Davidoff - Memphis TN, US
Assignee:
UCL BUSINESS PLC - London ST. JUDE CHILDREN'S RESEARCH HOSPITAL - Memphis TN THROMBOSIS RESEARCH INSTITUTE - Chelsea
An optimized coding sequence of human blood clotting factor eight (VIII) and a promoter may be used in vectors, such as rAAV, for introduction of factor VIII, and/or other blood clotting factors and transgenes. Exemplary of these factors and transgenes are alpha-1-antitrypsin, as well as those involved in the coagulation cascade, hepatocye biology, lysosomal storage, urea cycle disorders, and lipid storage diseases. Cells, vectors, proteins, and glycoproteins produced by cells transformed by the vectors and sequence, may be used in treatment.
Method Of Detecting Tumors Containing Complexes Of P53 And Hsp70
Jeffrey Robert Marks - Hillsborough NC James Dirk Inglehart - Durham NC Andrew Mark Davidoff - Durham NC Jerry G. Henslee - Libertyville IL
Assignee:
Duke University - Duram NC
International Classification:
G01N 33574 G01N 3353
US Classification:
435 723
Abstract:
The present invention relates to the finding that the class of mutant p53 proteins which bind to HSP70 defines a group of mutant p53 proteins which elicit serum autoantibodies. Thus, disclosed is a method of classifying tumor cells for the ability to produce serum p53 autoantibodies in a patient carrying such tumor cells, a method of detecting tumor cells containing a mutant p53 protein capable of forming a complex with a 70 kilodalton heat shock protein (hsp70) in the cells in a patient, a method of distinguishing tumor cells capable of causing more aggressive disease in a patient carrying the tumor cells, and a method of monitoring a patient for the recurrence of disease in a patient previously diagnosed as carrying tumor cells.
The invention provides improved adeno-associated virus (AAV) Factor VIII (FVIII) vectors, including AAV FVIII vectors that produce a functional Factor VIII polypeptide and AAV FVIII vectors with high expression activity.
An optimized coding sequence of human blood clotting factor eight (VIII) and a promoter may be used in vectors, such as rAAV, for introduction of factor VIII, and/or other blood clotting factors and transgenes. Exemplary of these factors and transgenes are alpha-1-antitrypsin, as well as those involved in the coagulation cascade, hepatocyte biology, lysosomal storage, urea cycle disorders, and lipid storage diseases. Cells, vectors, proteins, and glycoproteins produced by cells transformed by the vectors and sequence, may be used in treatment.
- London, GB - Memphis TN, US Edward Tuddenham - London, GB Andrew Davidoff - Memphis TN, US
International Classification:
A61K 38/37 C07K 14/755 A01K 67/027
Abstract:
There is provided a nucleic acid molecule comprising a nucleotide sequence encoding for a functional factor VIII protein, wherein the portion of the nucleotide sequence encoding for the B domain of the factor VIII protein is between 90 and 111 nucleotides in length and encodes for an amino acid sequence comprising a sequence having at least 85% identity to SEQ ID NO: 4 and which comprises six asparagine residues. Also provided is a functional factor VIII protein, a vector comprising the above nucleic acid molecule, a host cell, a transgenic animal, a method of treating haemophilia, e.g. haemophilia A, and a method for the preparation of a parvoviral gene delivery vector.
- London, GB - Memphis TN, US Edward Tuddenham - London, GB Andrew Davidoff - Memphis TN, US
International Classification:
A61K 38/37 C07K 14/755 A01K 67/027 A61K 48/00
Abstract:
There is provided a nucleic acid molecule comprising a nucleotide sequence encoding for a functional factor VIII protein, wherein the portion of the nucleotide sequence encoding for the B domain of the factor VIII protein is between 90 and 111 nucleotides in length and encodes for an amino acid sequence comprising a sequence having at least 85% identity to SEQ ID NO: 4 and which comprises six asparagine residues. Also provided is a functional factor VIII protein, a vector comprising the above nucleic acid molecule, a host cell, a transgenic animal, a method of treating haemophilia, e.g. haemophilia A, and a method for the preparation of a parvoviral gene delivery vector.
An optimized coding sequence of human blood clotting factor eight (VIII) and a promoter may be used in vectors, such as rAAV, for introduction of factor VIII, and/or other blood clotting factors and transgenes. Exemplary of these factors and transgenes arc alpha-1-antitrypsin, as well as those involved in the coagulation cascade, hepatocye biology, lysosomal storage, urea cycle disorders, and lipid storage diseases. Cells, vectors, proteins, and glycoproteins produced by cells transformed by the vectors and sequence, may be used in treatment.
- London, GB - Memphis TN, US Edward Tuddenham - London, GB Andrew Davidoff - Memphis TN, US
International Classification:
A61K 38/37
Abstract:
There is provided a nucleic acid molecule comprising a nucleotide sequence encoding for a functional factor VIII protein, wherein the portion of the nucleotide sequence encoding for the B domain of the factor VIII protein is between 90 and 111 nucleotides in length and encodes for an amino acid sequence comprising a sequence having at least 85% identity to SEQ ID NO: 4 and which comprises six asparagine residues. Also provided is a functional factor VIII protein, a vector comprising the above nucleic acid molecule, a host cell, a transgenic animal, a method of treating haemophilia, e.g. haemophilia A, and a method for the preparation of a parvoviral gene delivery vector.
Medicine Doctors
Dr. Andrew M Davidoff, Memphis TN - MD (Doctor of Medicine)
SAINT JUDE CHILDRENS HOSPITAL 262 Danny Thomas Pl, Memphis, TN 38105 901-495-3345 (Phone), 901-495-2270 (Fax)
Procedures:
Spinal Nerve Block
Conditions:
Hodgkin's Disease Osteosarcoma
Certifications:
General Surgery, 2005 Pediatric Surgery, 1998
Awards:
Healthgrades Honor Roll
Languages:
English
Education:
Medical School Perelman School of Medicine University of Pennsylvania Graduated: 1987 Medical School Duke University Hospital Graduated: 1987 Medical School Childrens Hospital Of Philadelphia Graduated: 1987
Sjcrh
262 Danny Thomas Pl, Memphis, TN 38105 Saint Jude Children's Research Hospital
332 N Lauderdale St, Memphis, TN 38105 Pediatric Surgical Group
777 Washington Ave, Memphis, TN 38105
262 Danny Thomas Pl, Memphis, TN 38105 332 N Lauderdale St, Memphis, TN 38105
Education:
University of Pennsylvania, Perelman School of Medicine - Doctor of Medicine Children's Hospital of Philadelphia, The - Fellowship - General Thoracic Surgery Duke University Hospital - Residency - Surgery
Board certifications:
American Board of Surgery Certification in Surgery American Board of Surgery Sub-certificate in Pediatric Surgery (Surgery)