Amy L Ligani

US Patent:

20070161052, Jul 12, 2007

Filed:

Oct 19, 2006

Appl. No.:

11/583086

Inventors:

Guy Servant - San Diego CA, US
Mark Williams - Carlsbad CA, US
Andrew Patron - San Marcos CA, US
Poonit Kamdar - San Diego CA, US
Qing Chen - San Diego CA, US
Bryan Moyer - San Diego CA, US
David Dahan - Oceanside CA, US
Tanya Ditschun - San Diego CA, US
Sumita Ray - San Diego CA, US
Amy Ligani - San Diego CA, US

Assignee:

SENOMYX, INC. - LaJolla CA

International Classification:

G01N 33/567

US Classification:

435007200

Abstract:

Robust cell based assays are provided that use cells that express wild-type or modified TRPM5 nucleic acid sequences in order to identify putative taste modulators, preferably sweet, bitter and umami taste modulators. The preferred assays use HEK-293 cells that express TRPM5, optionally at least one GPCR, preferably a taste specific GPCR, and a G protein that couples therewith. These assays detect TRPM5 modulators by use of membrane potential dyes that emit fluorescence on changes in TRPM5 activity based on changes in membrane potential and these changes in fluorescence are detectable using Fluorimetric Imaging Plate Readers (FLIPR).