Albert J Libchaber

US Patent:

6489106, Dec 3, 2002

Filed:

Mar 10, 2000

Appl. No.:

09/523578

Inventors:

G. V. Shivashankar - Bangalore, IN
Shumo Liu - Princeton NJ
Albert J. Libchaber - New York NY

Assignee:

NEC Research Institute, Inc. - Princeton NJ

International Classification:

C12Q 168

US Classification:

435 6, 435 911, 435 912, 4352852, 4352872, 536 221, 536 231, 536 241, 536 242, 536 243, 536 2433

Abstract:

The present invention demonstrates that gene expression can be controlled in vitro using DNA (gene) sequences immobilized on a template with micron scale temperature heaters. Such expression is controllable by varying temperature of the template on a short time scale. The present invention further demonstrates that nucleic acid constructs controlled by the present method express protein either free or bound to the nucleic acid. Based on these findings, the present invention provides methods and apparatuses useful for the preparation of in vitro programmable protein networks and protein micro arrays.

US Patent:

7939170, May 10, 2011

Filed:

Aug 15, 2002

Appl. No.:

10/219440

Inventors:

Benoit Dubertret - New York NY, US
Vincent Noireaux - New York NY, US
Albert Libchaber - New York NY, US

Assignee:

The Rockefeller University - New York NY

International Classification:

A61K 9/50

US Classification:

42840224, 428403, 436 73, 436829, 424450, 427215, 427220

Abstract:

The invention provides a water soluble complex comprising an inner core of a metal or semi-conductor nanoparticle. The nanoparticle is coated with a hydrophobic ligand, which is encapsulated in a micelle. In an aqueous medium, the micelle comprises a hydrophilic shell and a hydrophobic core, the hydrophilic shell comprising a plurality of hydrophilic moieties, the hydrophobic core comprising a plurality of hydrophobic moieties, each hydrophobic moiety comprising at least one chain, each chain comprising a minimum of 8 atoms; wherein the total number of atoms in all chains for each moiety comprises at least 24 atoms. The micelle has a minimum average diameter of approximately 5 nm and a maximum average diameter of approximately 45 nm.

US Patent:

7951453, May 31, 2011

Filed:

Oct 31, 2007

Appl. No.:

11/981439

Inventors:

Benoit Dubertret - New York NY, US
Vincent Noireaux - New York NY, US
Albert Libchaber - New York NY, US

Assignee:

The Rockefeller University - New York NY

International Classification:

A61K 9/50

US Classification:

42840224, 428403, 436 73, 436829, 424450, 427215, 427220

Abstract:

The invention provides a water soluble complex comprising an inner core of a metal or semi-conductor nanoparticle. The nanoparticle is coated with a hydrophobic ligand, which is encapsulated in a micelle. In an aqueous medium, the micelle comprises a hydrophilic shell and a hydrophobic core, the hydrophilic shell comprising a plurality of hydrophilic moieties, the hydrophobic core comprising a plurality of hydrophobic moieties, each hydrophobic moiety comprising at least one chain, each chain comprising a minimum of 8 atoms; wherein the total number of atoms in all chains for each moiety comprises at least 24 atoms. The micelle has a minimum average diameter of approximately 5 nm and a maximum average diameter of approximately 45 nm.

US Patent:

20020055146, May 9, 2002

Filed:

Dec 7, 2001

Appl. No.:

10/012793

Inventors:

G. Shivashankar - Bangalore, IN
Shumo Liu - Princeton NJ, US
Albert Libchaber - New York NY, US

International Classification:

C12P021/06
C12P019/34

US Classification:

435/069100, 435/091200

Abstract:

The present invention demonstrates that gene expression can be controlled in vitro using DNA (gene) sequences immobilized on a template with micron scale temperature heaters. Such expression is controllable by varying temperature of the template on a short time scale. The present invention further demonstrates that nucleic acid constructs controlled by the present method express protein either free or bound to the nucleic acid. Based on these findings, the present invention provides methods and apparatuses useful for the preparation of in vitro programmable protein networks and protein micro arrays.

US Patent:

20040002089, Jan 1, 2004

Filed:

Feb 26, 2003

Appl. No.:

10/374686

Inventors:

Benoit Dubertret - New York NY, US
Michel Calame - Riehen, CH
Albert Libchaber - New York NY, US

International Classification:

C12Q001/68
G01N033/553

US Classification:

435/006000, 436/525000

Abstract:

The invention is broadly directed to methods for sensitively detecting proximity changes in systems that utilizes an interacting fluorophore and quencher. In such methods, a metal surface is used as the quencher. The metal surface may be a particle or film, such as nanoparticles or a coating, respectively. Such systems provide an increase in sensitivity over previously-described quenchers, offering a signal-to-noise ratio of up to several orders of magnitude. Examples of such systems in which proximity changes are usefully detected include conformational changes in biomolecules resulting from their interaction with their binding partners or ligands. Such biomolecules may be, for example, nucleic acids, proteins, peptides, polysaccharides, or other polymeric, naturally occurring or synthetic molecules. These include, by way of non-limiting example, molecular beacons, which detect particular polynucleotide sequences; antibody-antigen interactions, and conformational changes in proteins upon binding to a ligand or substrate.

US Patent:

61398314, Oct 31, 2000

Filed:

May 28, 1998

Appl. No.:

9/086192

Inventors:

Albert J. Libchaber - New York NY

Assignee:

The Rockfeller University - New York NY

International Classification:

G01T 120
C09K 300
G01B 1116
G01N 100
G01N 110

US Classification:

424 8205

Abstract:

Apparatus and method for immobilizing molecules, particularly biomolecules such as DNA, RNA, proteins, lipids, carbohydrates, or hormones onto a substrate such as glass or silica; patterns of immobilization can be made resulting in addressable, discrete arrays of molecules on a substrate, having applications in bioelectronics, DNA hybridization assays, drug assays, etc.