Quan H Nguyen

US Patent:

7049151, May 23, 2006

Filed:

Mar 6, 2002

Appl. No.:

10/092926

Inventors:

Quan Nguyen - Pleasant Hill CA, US
Yong Song - San Pablo CA, US

Assignee:

Bio-Rad Laboratories, Inc. - Hercules CA

International Classification:

G01N 33/543

US Classification:

436518, 436523, 436524, 435 71, 435 792, 435973

Abstract:

A method and kit for simultaneous detection and/or determination of a plurality of modified proteins in a sample. The method comprises: a) contacting the sample under mild protein denaturation conditions with a plurality of first antibodies capable of binding to a specific target protein, the first antibodies being immobilized on solid support material, each first antibody being differentiable from others by a differentiation parameter, whereby the first antibodies bind to respective target proteins present in the sample; b) removing unbound materials from the locus of the first antibodies; c) contacting the materials from step (b) with one or more second antibodies, each of which is specific to a class or subclass of modified proteins or with a plurality of second antibodies, each of which is specific to a modified protein, so as to bind the second antibody or antibodies to modified proteins in the sample; and d) detecting and/or determining a plurality of modified proteins in the sample. The kit comprises a plurality of primary antibodies immobilized on solid support material, one or more buffers for lysing and for washing cellular material samples to be assayed, an assay buffer for conducting the assay, the buffer containing a sulfate or sulfonate detergent, and one or more second antibodies specific to the modified proteins.

US Patent:

7271886, Sep 18, 2007

Filed:

Nov 17, 2003

Appl. No.:

10/716176

Inventors:

Frank Witney - Oakland CA, US
Gary McMaster - Ann Arbor MI, US
Quan Nguyen - San Ramon CA, US
Steve Chen - Fremont CA, US

Assignee:

Panomics, Inc. - Fremont CA

International Classification:

G01J 1/00

US Classification:

356213, 356218, 435 5, 2504581

Abstract:

Uncaging devices that can be used to uncage photoactivatable caged components are provided. Consistent, uniform and/or high throughput processing of reactions and assays that include caged components is provided. Masked multiwell plates that can be used for uncaging photoactivatable caged components are provided. Methods and apparatus for initiating assays involving caged components are provided.

US Patent:

7376032, May 20, 2008

Filed:

Jun 1, 2006

Appl. No.:

11/421497

Inventors:

Lam Van Nguyen - Cupertino CA, US
Quan Nguyen - Cupertino CA, US

Assignee:

QUALCOMM Incorporated - San Diego CA

International Classification:

G11C 7/02

US Classification:

365210, 365214, 36518909

Abstract:

A dummy SRAM cell for use in a dummy bit line circuit uses the same transistors as used in a standard SRAM cell, which includes first and second subsets of transistors configured as first and second bit line output circuits. The dummy SRAM cell includes the same first and second subsets of transistors, with the first transistors configured as a dummy bit line output circuit having substantially the same electrical characteristics as the first bit line output circuit of the standard SRAM cell. Further, the second transistors, which are not otherwise needed for the dummy SRAM cell function, are reconfigured as a voltage tie circuit for the dummy bit line output. Using the second transistors for this purpose obviates the need to add additional transistors to form a voltage tie circuit for configuring the dummy bit line output circuit as a load or driver for the dummy bit line.

US Patent:

7541193, Jun 2, 2009

Filed:

Nov 17, 2003

Appl. No.:

10/716174

Inventors:

Quan Nguyen - San Ramon CA, US
Gary McMaster - Ann Arbor MI, US

Assignee:

Panomics, Inc. - Fremont CA

International Classification:

G01N 21/76

US Classification:

436172, 436164, 435183, 435193

Abstract:

A number of caged (e. g. , photoactivatable) compositions are provided, for example, caged enzyme sensors, caged binding sensors, caged nucleic acid probes, caged interfering RNAs, caged antisense nucleic acids, caged ribozymes, caged biomolecular analogs, caged transcription factors, caged antibodies, caged molecular decoys, and caged aptamers. Compositions comprising a plurality of caged components are also provided, as are labeled and optionally caged modulators. Also provided are kits for making the caged compositions and methods for making and for using the caged compositions. Related systems and devices are also provided (e. g. , an uncaging device that can trigger photoactivatable assays and optionally detect signals from the assays).

US Patent:

7551271, Jun 23, 2009

Filed:

Aug 13, 2007

Appl. No.:

11/893012

Inventors:

Frank Witney - Oakland CA, US
Gary McMaster - Ann Arbor MI, US
Quan Nguyen - San Ramon CA, US
Steve Chen - Fremont CA, US

Assignee:

Panomics, Inc. - Fremont CA

International Classification:

G01J 1/00

US Classification:

356213, 356218, 435 5, 2504581

Abstract:

Uncaging devices that can be used to uncage photoactivatable caged components are provided. Consistent, uniform and/or high throughput processing of reactions and assays that include caged components is provided. Masked multiwell plates that can be used for uncaging photoactivatable caged components are provided. Methods and apparatus for initiating assays involving caged components are provided.

US Patent:

20020115116, Aug 22, 2002

Filed:

Feb 22, 2001

Appl. No.:

09/792404

Inventors:

Yong Song - San Pablo CA, US
Quan Nguyen - Pleasant Hill CA, US

International Classification:

G01N033/53

US Classification:

435/007900

Abstract:

Fusion proteins in which glutathione S-transferase (GST) is a fusion partner are used as an immobilized binding member in screening procedures or other multi-analyte test procedures based on protein interaction. In such procedures, a particular protein is selected from a group of candidate proteins on the basis of the binding affinity of that protein for a target protein, with either the candidate proteins or the target protein being a fusion partner with GST and the GST portion of the fusion partner having been immobilized on glutathione-coated particles by the binding of GST to glutathione. The particles themselves are classifiable by different values of a differentiation parameter that permits them to be distinguished by flow cytometry, and the procedure is conducted in a manner that associates the individual candidate proteins with individual classes of the particles. When a binding interaction occurs between a candidate protein and the target protein, the particles on which the interaction has occurred are readily distinguished by flow cytometry and correlated with the candidate protein that exhibited the binding.

US Patent:

20020137097, Sep 26, 2002

Filed:

Dec 13, 2001

Appl. No.:

10/017788

Inventors:

Quan Nguyen - Pleasant Hill CA, US
Aiguo Zhang - Hayward CA, US

Assignee:

Bio-Rad Laboratories, Inc. - Hercules CA

International Classification:

G01N033/53
G01N033/543

US Classification:

435/007100, 436/518000

Abstract:

The present invention provides standard diluents for use in multiplex assays comprising a biological fluid that normally includes two or more different target analytes but that is substantially free of the two or more different target analytes, as well as kits and methods relating to the standard diluent. The standard diluents of the invention provide an accurate and reliable means to calibrate the amount of multiple target analytes in a single assay.

US Patent:

20030013116, Jan 16, 2003

Filed:

Sep 13, 2002

Appl. No.:

10/243099

Inventors:

Yong Song - San Pablo CA, US
Quan Nguyen - Pleasant Hill CA, US

Assignee:

Bio-Rad Laboratories, Inc. - Hercules CA

International Classification:

C12Q001/68
G01N033/53
G01N033/542
G01N033/537
G01N033/543

US Classification:

435/006000, 435/007500, 435/007920

Abstract:

Fusion proteins in which glutathione S-transferase (GST) is a fusion partner are used as an immobilized binding member in screening procedures or other multi-analyte test procedures based on protein interaction. In such procedures, a particular protein is selected from a group of candidate proteins on the basis of the binding affinity of that protein for a target protein, with either the candidate proteins or the target protein being a fusion partner with GST and the GST portion of the fusion partner having been immobilized on glutathione-coated particles by the binding of GST to glutathione. The particles themselves are classifiable by different values of a differentiation parameter that permits them to be distinguished by flow cytometry, and the procedure is conducted in a manner that associates the individual candidate proteins with individual classes of the particles. When a binding interaction occurs between a candidate protein and the target protein, the particles on which the interaction has occurred are readily distinguished by flow cytometry and correlated with the candidate protein that exhibited the binding.